cDNA Clone VAPB

• Synonyms: VAP-B , ALS8
• Plasmid: pET28a- VAPB aa1-210 K87D M89D -TEV-HALO (C ter)
• Parent Plasmid: pET28a original
• Expressed: VAPB aa1-210 K87D M89D - HALO
• Genbank: NM_004738.3
• Tag: HALO
• Species: Human

Sequence of Insert

CCATGGGTCGCGGATCCATGGCGAAGGTGGAGCAGGTCCTGAGCCTCGAG
CCGCAGCACGAGCTCAAATTCCGAGGTCCCTTCACCGATGTTGTCACCAC
CAACCTAAAGCTTGGCAACCCGACAGACCGAAATGTGTGTTTTAAGGTGA
AGACTACAGCACCACGTAGGTACTGTGTGAGGCCCAACAGCGGAATCATC
GATGCAGGGGCCTCAATTAATGTATCTGTGATGTTACAGCCTTTCGATTA
TGATCCCAATGAGAAAAGTAAACACGACTTTGACGTTCAGTCTATGTTTG
CTCCAACTGACACTTCAGATATGGAAGCAGTATGGAAGGAGGCAAAACCG
GAAGACCTTATGGATTCAAAACTTAGATGTGTGTTTGAATTGCCAGCAGA
GAATGATAAACCACATGATGTAGAAATAAATAAAATTATATCCACAACTG
CATCAAAGACAGAAACACCAATAGTGTCTAAGTCTCTGAGTTCTTCTTTG
GATGACACCGAAGTTAAGAAGGTTATGGAAGAATGTAAGAGGCTGCAAGG
TGAAGTTCAGAGGCTACGGGAGGAGAACAAGCAGTTCAAGGAAGAAGATG
GACTGCGGATGAGGAAGACAGTGCAGAGCAACAGCCCCATTTCAGCAGAA
TTCGAGCTCCGTCGACaagcttccGAAAACCTGTATTTTCAGGGCatggc
agaaatcggtactggctttccattcgacccccattatgtggaagtcctgg
gcgagcgcatgcactacgtcgatgttggtccgcgcgatggcacccctgtg
ctgttcctgcacggtaacccgacctcctcctacgtgtggcgcaacatcat
cccgcatgttgcaccgacccatcgctgcattgctccagacctgatcggta
tgggcaaatccgacaaaccagacctgggttatttcttcgacgaccacgtc
cgcttcatggatgccttcatcgaagccctgggtctggaagaggtcgtcct
ggtcattcacgactggggctccgctctgggtttccactgggccaagcgca
atccagagcgcgtcaaaggtattgcatttatggagttcatccgccctatc
ccgacctgggacgaatggccagaatttgcccgcgagaccttccaggcctt
ccgcaccaccgacgtcggccgcaagctgatcatcgatcagaacgttttta
tcgagggtacgctgccgatgggtgtcgtccgcccgctgactgaagtcgag
atggaccattaccgcgagccgttcctgaatcctgttgaccgcgagccact
gtggcgcttcccaaacgagctgccaatcgccggtgagccagcgaacatcg
tcgcgctggtcgaagaatacatggactggctgcaccagtcccctgtcccg
aagctgctgttctggggcaccccaggcgttctgatcccaccggccgaagc
cgctcgcctggccaaaagcctgcctaactgcaaggctgtggacatcggcc
cgggtctgaatctgctgcaagaagacaacccggacctgatcggcagcgag
atcgcgcgctggctgtcgacgctcgagatttccggcTGAgcggccgc

Amino Acid Sequence

MGRGSMAKVEQVLSLEPQHELKFRGPFTDVVTTNLKLGNPTDRNVCFKVK
TTAPRRYCVRPNSGIIDAGASINVSVMLQPFDYDPNEKSKHDFDVQSMFA
PTDTSDMEAVWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINKIISTTA
SKTETPIVSKSLSSSLDDTEVKKVMEECKRLQGEVQRLREENKQFKEEDG
LRMRKTVQSNSPISAEFELRRQASENLYFQGMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISG*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU24181
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 5 additional amino acids (MGRGS) on N-terminus prior to native start