cDNA Clone TRPM6

• Synonyms: CHAK2, HMGX, HOMG, HOMG1, HSH
• Plasmid: pcDNA5frtTO FLAG TRPM6 S1754N
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG-TRPM6 S1754N
• Genbank: NM_017662.4
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGAAAGAACAACCTGTCTTGGAGCGCTTGCAGTCCCAGAAATCCTGGATTAAAGGAGTATTTGACAAGAGAGAATGTAGCACAATCATACCCAGCTCAAAAAATCCTCACAGATGTACTCCAGTATGCCAAGTCTGCCAGAATTTAATCAGGTGTTACTGTGGCCGACTGATTGGAGACCATGCTGGGATAGATTATTCCTGGACCATCTCAGCTGCCAAGGGTAAAGAAAGTGAACAATGGTCTGTTGAAAAGCACACAACGAAAAGCCCAACAGATACTTTTGGCACGATTAATTTCCAAGATGGAGAGCACACCCATCATGCCAAGTATATTAGAACTTCTTATGATACAAAACTGGATCATCTGTTACATTTAATGTTGAAAGAGTGGAAAATGGAACTGCCCAAGCTTGTGATCTCAGTCCATGGGGGCATCCAGAACTTTACTATGCCCTCTAAATTTAAAGAGATTTTCAGCCAAGGTTTGGTTAAAGCTGCAGAGACAACCGGTGCGTGGATAATAACTGAAGGCATCAATACAGGAGTGTCCAAGCATGTTGGGGATGCCTTGAAATCCCATTCCTCTCATTCCTTGAGAAAAATCTGGACAGTTGGAATCCCTCCTTGGGGTGTCATTGAGAACCAGAGAGACCTTATTGGAAAAGATGTGGTGTGCCTGTACCAGACTCTGGATAACCCCCTCAGCAAGCTCACAACACTCAACAGCATGCACTCGCACTTCATCCTGTCTGATGATGGGACCGTGGGCAAGTATGGAAATGAAATGAAGCTCAGAAGGAACCTGGAGAAGTACCTCTCTCTGCAGAAAATACACTGCCGCTCAAGACAAGGCGTGCCGGTCGTGGGGCTGGTGGTGGAAGGCGGTCCCAACGTCATCCTGTCAGTGTGGGAGACTGTCAAGGACAAGGACCCAGTGGTGGTGTGTGAGGGTACAGGTAGGGCGGCTGACCTCCTGGCCTTCACACACAAACACCTGGCAGATGAAGGGATGCTGCGACCTCAGGTGAAAGAGGAGATCATCTGCATGATTCAGAACACTTTCAACTTTAGTCTTAAACAGTCCAAGCACCTTTTCCAAATTCTAATGGAGTGTATGGTTCACAGGGATTGTATTACCATATTTGATGCTGACTCTGAAGAGCAGCAAGACCTGGACTTAGCAATCCTAACAGCTTTGCTGAAGGGCACAAATTTATCAGCGTCAGAGCAATTAAATCTGGCAATGGCTTGGGACAGGGTGGACATTGCCAAGAAACATATCCTAATTTATGAACAACACTGGAAGCCTGATGCCCTGGAACAAGCAATGTCAGATGCTTTAGTGATGGATCGGGTGGATTTTGTGAAGCTCTTAATAGAATATGGAGTGAACCTCCATCGCTTTCTTACCATCCCTCGACTGGAAGAGCTCTACAATACAAAACAAGGACCTACTAATACACTCTTGCATCATCTCGTCCAAGATGTGAAACAGCATACCCTTCTTTCAGGCTACCGAATAACCTTGATTGACATTGGATTAGTAGTAGAATACCTCATTGGTAGAGCATATCGCAGCAACTACACTAGAAAACATTTCAGAGCCCTCTACAACAACCTCTACAGAAAATACAAGCACCAGAGACACTCCTCAGGAAATAGAAATGAGTCTGCAGAAAGTACGCTGCACTCCCAGTTCATTAGAACTGCACAGCCATACAAATTCAAGGAAAAGTCTATAGTCCTTCATAAATCAAGGAAGAAGTCAAAAGAACAAAATGTATCAGATGACCCTGAGTCTACTGGCTTTCTTTACCCTTACAATGACCTGCTGGTTTGGGCTGTGCTGATGAAAAGGCAGAAGATGGCTATGTTCTTCTGGCAGCATGGAGAGGAGGCCACGGTTAAAGCCGTGATTGCGTGTATCCTCTACCGGGCAATGGCCCATGAAGCTAAGGAGAGTCACATGGTGGATGATGCCTCAGAAGAGTTGAAGAATTACTCAAAACAGTTTGGCCAGCTGGCTCTGGACTTGTTGGAGAAGGCATTCAAGCAGAATGAGCGCATGGCCATGACGCTGTTGACGTATGAACTCAGGAACTGGAGCAATTCGACCTGCCTTAAACTGGCCGTGTCGGGAGGATTACGACCCTTTGTTTCACATACTTGTACCCAGATGCTACTGACAGACATGTGGATGGGGAGGCTGAAAATGAGGAAAAACTCTTGGTTAAAGATTATTATAAGCATTATTTTACCACCCACCATTTTGACACTGGAATTTAAAAGCAAAGCTGAGATGTCACATGTTCCCCAGTCCCAGGACTTCCAATTTATGTGGTATTACAGTGACCAGAACGCCAGCAGTTCCAAAGAAAGTGCTTCTGTGAAAGAGTATGATTTGGAAAGGGGCCATGATGAGAAACTGGATGAAAATCAGCATTTTGGTTTGGAAAGTGGGCACCAACACCTTCCGTGGACCAGGAAAGTCTATGAGTTCTACAGTGCTCCAATTGTCAAGTTTTGGTTTTATACGATGGCGTATTTGGCATTCCTCATGCTGTTCACTTACACCGTGTTGGTGGAGATGCAGCCCCAGCCCAGCGTGCAGGAGTGGCTTGTTAGCATTTACATCTTCACCAATGCTATTGAGGTGGTCAGGGAGATCTGTATTTCAGAACCTGGGAAGTTTACCCAAAAGGTGAAGGTATGGATTAGTGAGTACTGGAACTTAACAGAAACTGTGGCCATTGGCCTGTTTTCAGCTGGCTTCGTCCTTCGATGGGGTGACCCTCCTTTTCACACAGCGGGAAGACTGATCTACTGCATAGACATCATATTCTGGTTCTCACGGCTCCTGGACTTCTTTGCTGTGAATCAACATGCAGGTCCATATGTGACCATGATTGCAAAAATGACAGCAAACATGTTCTATATTGTGATCATCATGGCCATAGTCCTGCTGAGCTTTGGAGTGGCACGCAAGGCCATCCTTTCGCCAAAAGAGCCACCATCTTGGAGTCTNNCNCNANATATTGTATTTGAGCCATACTGGATGATATACGGAGAAGTCTATGCTGGAGAAATAGATGTTTGTTCAAGCCAGCCATCCTGCCCTCCTGGTTCTTTTCTTACTCCATTCTTGCAAGCTGTCTACCTCTTCGTGCAATATATCATCATGGTGAACCTGTTGATTGCTTTCTTCAACAACGTTTACTTAGATATGGAATCCATTTCAAATAACCTGTGGAAATACAACCGCTATCGCTACATCATGACCTACCACGAGAAGCCCTGGCTGCCCCCACCTCTCATCCTGCTGAGCCACGTGGGCCTTCTCCTCCGCCGCCTGTGCTGTCATCGAGCTCCTCACGACCAAGAAGAGGGTGACGTTGGATTAAAACTCTACCTCAGTAAGGAGGATCTGAAAAAACTTCATGATTTTGAGGAGCAGTGCGTGGAAAAATACTTCCATGAGAAGATGGAAGATGTGAATTGTAGTTGTGAGGAACGAATCCGAGTGACATCAGAAAGGGTTACAGAGATGTACTTCCAGCTGAAAGAAATGAATGAAAAGGTGTCTTTTATAAAGGACTCCTTACTGTCTTTGGACAGCCAGGTGGGACACCTGCAGGATCTCTCTGCCCTGACTGTGGATACCCTGAAAGTCCTTTCTGCTGTTGACACTTTGCAAGAGGATGAGGCTCTCCTGGCCAAGAGAAAGCATTCTACTTGCAAAAAACTTCCCCACAGCTGGAGCAATGTCATCTGTGCAGAGGTTCTAGGCAGCATGGAGATCGCTGGAGAGAAGAAATACCAGTATTATAGCATGCCCTCTTCTTTGCTGAGGAGCCTGGCTGGAGGCCGGCATCCCCCAAGAGTGCAGAGGGGGGCACTTCTTGAGATTACAAACAGTAAAAGAGAGGCTACAAATGTAAGAAATGACCAGGAAAGGCAAGAAACACAAAGTAGTATAGTGGTTTCTGGGGTGTCTCCTAACAGGCAAGCACACTCAAAGTATGGCCAGTTTCTTCTGGTCCCCTCTAATCTAAAGCGAGTTCCTTTTTCAGCAGAAACTGTCTTGCCTCTGTCCAGACCCTCTGTGCCAGATGTGCTGGCAACTGAACAGGACATCCAGACTGAGGTTCTTGTTCATCTGACTGGGCAGACCCCAGTTGTCTCTGACTGGGCATCAGTGGATGAACCCAAGGAAAAGCACGAGCCTATTGCTCACTTACTGGATGGACAAGACAAGGCAGAGCAAGTGCTACCCACTTTGAGTTGCACACCTGAACCCATGACAATGAGCTCCCCTCTTTCCCAAGCCAAGATCATGCAAACTGGAGGTGGATATGTAAACTGGGCATTTTCAGAAGGTGATGAAACTGGTGTGTTTAGCATCAAGAAAAAGTGGCAAACCTGCTTGCCCTCCACTTGTGACAGTGATTCCTCTCGGAGTGAACAGCACCAGAAGCAGGCCCAGGACAGCTCCCTATCTGATAACTCAACAAGATCGGCCCAGAGTAGTGAATGCTCAGAGGTGGGACCATGGCTTCAGCCAAACACATCCTTTTGGATCAATCCTCTCCGCAGATACAGGCCCTTCGCTAGGAGTCATAGTTTTAGATTCCATAAGGAGGAGAAATTGATGAAGATCTGTAAGATTAAAAATCTTTCAGGCTCTTCAGAAATAGGGCAGGGAGCATGGGTCAAAGCGAAAATGCTAACCAAAGACAGGAGACTGTCAAAGAAAAAGAAGAATACTCAAGGACTCCAGGTGCCAATCATAACAGTCAATGCCTGCTCTCAGAGTGACCAGTTGAATCCAGAGCCAGGAGAAAACAGCATCTCTGAAGAGGAGTACAGCAAGAACTGGTTCACAGTGTCCAAATTTAGTCACACAGGTGTAGAACCTTACATACATCAGAAAATGAAAACTAAAGAAATTGGACAATGTGCTATACAAATCAGTGATTACCTAAAGCAGTCTCAAGAGGATCTCAGCAAAAACTCTTTGTGGAATTCCAGGAGCACCAACCTCAATAGGAACTCCCTGCTGAAAAGTTCAATTGGAGTTGACAAGATCTCAGCCTCCTTAAAAAGCCCTCAAGAGCCTCACCATCATTATTCAGCCATTGAAAGGAATAATTTAATGAGGCTTTCTCAGACCATACCATTTACACCAGTCCAACTGTTTGCAGGAGAAGAAATAACCGTCTACAGGTTGGAGGAGAGTTCCCCTTTAAACCTTGATAAAAATATGTCCTCTTGGTCTCAGCGTGGGAGAGCGGCAATGATCCAGGTATTGTCCCGAGAGGAGATGGATGGGGGCCTCCGTAAAGCTATGAGAGTCGTCAGCACTTGGTCTGAGGATGACATTCTCAAGCCGGGACAAGTTTTCATTGTCAAGTCCTTTCTTCCTGAGGTTGTGCGGACATGGCATAAAATCTTCCAGGAGAGCACTGTGCTTCATCTTTGCCTCAGGGAAATTCAACAACAAAGGGCTGCTCAAAAATTGATCTATACCTTCAACCAAGTGAAACCACAAACCATACCCTACACACCAAGGTTCCTGGAAGTTTTCTTAATCTACTGCCATTCAGCCAACCAGTGGTTGACCATTGAGAAGTATATGACAGGGGAGTTCCGGAAGTATAACAACAACAATGGTGATGAAATCACCCCCACCAACACCCTGGAGGAGCTGATGTTGGCTTTCTCTCACTGGACCTATGAGTACACTCGGGGAGAGCTGCTGGTTTTAGATTTGCAAGGTGTTGGAGAAAATTTGACAGATCCATCTGTTATAAAACCTGAAGTCAAACAATCAAGAGGAATGGTGTTTGGACCGGCCAATTTGGGGGAAGATGCAATTAGAAACTTCATTGCAAAACATCATTGTAACTCCTGCTGCCGGAAGCTCAAACTCCCGGATTTAAAAAGAAATGACTATTCCCCTGAAAGGATAAATTCCACCTTTGGACTTGAGATAAAAATAGAATCAGCTGAGGAGCCTCCAGCAAGGGAGACGGGTAGAAATTCCCCAGAAGATGATATGCAACTATAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRC
TPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTK
SPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLV
ISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGD
ALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLT
TLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVV
GLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGML
RPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQ
QDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDA
LEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTL
LHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYN
NLYRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKK
SKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAV
IACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNER
MAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKM
RKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNAS
SSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPI
VKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVRE
ICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGR
LIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLL
SFGVARKAILSPKEPPSWSXXXXIVFEPYWMIYGEVYAGEIDVCSSQPSC
PPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRY
RYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLS
KEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKE
MNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLA
KRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGG
RHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHS
KYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTG
QTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSP
LSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQ
HQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARS
HSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNT
QGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEP
YIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKS
SIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEIT
VYRLEESSPLNLDKNMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTW
SEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQK
LIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNN
NGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKP
EVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPER
INSTFGLEIKIESAEEPPARETGRNSPEDDMQL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44830
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: S1754N; ; All TRPM6 plasmids MUST be grown at 30 degrees C or less to prevent recombination.