cDNA Clone TTBK2

• Synonyms: KIAA0847, SCA11, TTBK
• Plasmid: pET15b TEV HALO TTBK2 600-1100
• Parent Plasmid: pET15b 6His TEV Halo 3C
• Expressed: 6xHIS-TEV-HALO-TTBK2 600-1100
• Genbank: NM_173500.3
• Tag: 6His
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGTTAGGTCCTTGGGCAGAAAATGATCATTTAAAGAAGGAAACCTCAGGTGTGGTCTTAGCACTTTCTGCAGAGGGTCCTCCTACTGCTGCTTCAGAACAATATACAGATAGGCTGGAACTCCAGCCTGGAGCTGCTAGTCAGTTTATTGCAGCGACGCCCACAAGTCTAATGGAGGCGCAGGCAGAAGGACCCCTTACAGCGATTACAATTCCTAGACCTTCTGTGGCATCTACACAGTCAACTTCAGGAAGCTTTCACTGTGGTCAGCAGCCAGAGAAGAAAGATCTTCAGCCCATGGAGCCCACTGTGGAACTTTACTCTCCAAGGGAAAACTTCTCTGGCTTGGTTGTGACAGAGGGTGAACCTCCTAGTGGAGGAAGCAGAACAGATTTGGGGCTTCAGATAGATCACATTGGTCATGACATGTTACCCAACATTAGAGAAAGTAACAAATCTCAAGACCTGGGACCAAAAGAACTTCCTGATCATAATAGACTGGTTGTGAGAGAATTTGAAAATCTCCCTGGGGAAACTGAAGAGAAAAGCATCCTTTTAGAGTCAGATAATGAAGATGAGAAGTTAAGTAGAGGGCAGCATTGTATTGAGATCTCCTCTCTCCCAGGAGATTTGGTAATTGTGGAAAAGGATCACTCAGCTACTACTGAACCTCTTGATGTGACAAAAACACAGACTTTTAGTGTGGTGCCAAATCAAGACAAAAATAATGAGATAATGAAGCTTCTGACAGTTGGAACTTCAGAAATTTCTTCCAGAGACATTGACCCACATGTTGAAGGTCAGATAGGCCAAGTGGCAGAAATGCAAAAAAATAAGATATCTAAGGATGATGACATCATGAGTGAAGACTTGCCAGGTCATCAAGGAGACCTCTCTACTTTTTTGCACCAAGAGGGCAAGAGAGAGAAAATCACCCCTAGAAATGGAGAACTATTTCATTGTGTTTCAGAGAATGAACATGGTGCCCCAACCCGGAAGGATATGGTTAGGTCATCCTTTGTAACTAGACACAGCCGAATCCCTGTTTTAGCACAAGAGATAGACTCAACTTTGGAATCATCCTCTCCAGTTTCTGCAAAAGAAAAGCTCCTCCAAAAGAAAGCCTATCAGCCAGACCTAGTCAAGCTTCTGGTGGAAAAAAGACAATTCAAGTCCTTCCTTGGCGACCTCTCAAGTGCCTCTGATAAATTGCTAGAGGAGAAACTAGCTACTGTTCCTGCTCCCTTTTGTGAGGAGGAAGTGCTCACTCCCTTTTCAAGACTGACAGTAGATTCTCACCTGAGTAGGTCAGCTGAAGATAGCTTTCTGTCACCCATCATCTCCCAGTCTAGAAAGAGCAAAATTCCAAGGCCAGTTTCATGGGTCAACACAGATCAGGTCAATAGCTCAACTTCGTCTCAGTTCTTTCCTCGGCCACCACCAGGAAAGCCACCCACGAGGCCTGGAGTAGAAGCCAGGCTACGCAGATATAAAGTCCTAGGGAGTTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSENLYFQGMAEIGTGFPFDPHYVEVLGE
RMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMG
KSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNP
ERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIE
GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVA
LVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPG
LNLLQEDNPDLIGSEIARWLSTLEISGLEVLFQGPGSMLGPWAENDHLKK
ETSGVVLALSAEGPPTAASEQYTDRLELQPGAASQFIAATPTSLMEAQAE
GPLTAITIPRPSVASTQSTSGSFHCGQQPEKKDLQPMEPTVELYSPRENF
SGLVVTEGEPPSGGSRTDLGLQIDHIGHDMLPNIRESNKSQDLGPKELPD
HNRLVVREFENLPGETEEKSILLESDNEDEKLSRGQHCIEISSLPGDLVI
VEKDHSATTEPLDVTKTQTFSVVPNQDKNNEIMKLLTVGTSEISSRDIDP
HVEGQIGQVAEMQKNKISKDDDIMSEDLPGHQGDLSTFLHQEGKREKITP
RNGELFHCVSENEHGAPTRKDMVRSSFVTRHSRIPVLAQEIDSTLESSSP
VSAKEKLLQKKAYQPDLVKLLVEKRQFKSFLGDLSSASDKLLEEKLATVP
APFCEEEVLTPFSRLTVDSHLSRSAEDSFLSPIISQSRKSKIPRPVSWVN
TDQVNSSTSSQFFPRPPPGKPPTRPGVEARLRRYKVLGS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44807
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 600-1100. Use TEV to cleave off 6xHIS, precission may be used downstream to remove the HALO tag if necessary.