cDNA Clone UBQLN2

• Synonyms: DSK2, CHAP1, N4BP4, PLIC2, FLJ10167, FLJ56541, HRIHFB2157
• Plasmid: pcDNA5-FRT/TO- UBQLN2 siRNA resistant (3)
• Parent Plasmid: pcDNA5D FRT/TO
• Expressed: UBQLN2
• Genbank: Gene ID 29978
• Species: Human

Sequence of Insert

gaattcAGATCCATGGCTGAGAATGGCGAGAGCAGCGGCCCCCCGCGCCCCTCCCG
CGGCCCTGCTGCGGCCCAAGGCTCGGCTGCTGCCCCGGCTGAGCCTAAAA
TCATCAAAGTCACGGTGAAGACTCCCAAAGAGAAAGAGGAGTTCGCGGTG
CCCGAGAACAGCTCGGTTCAGCAGTTTAAGGAAGCGATTTCGAAACGCTT
CAAATCCCAAACCGATCAGCTAGTGCTGATTTTTGCCGGAAAAATCTTAA
AAGATCAAGATACCTTGATCCAGCATGGCATCCATGATGGGCTGACTGTT
CACCTTGTCATCAAAAGCCAGAACCGACCTCAGGGCCAGTCCACGCAGCC
TAGCAATGCCGCGGGAACTAACACTACCTCGGCGTCGACTCCCAGGAGTA
ACTCCACACCTATTTCCACAAATAGCAACCCGTTTGGGTTGGGGAGCCTG
GGAGGACTTGCAGGCCTTAGCAGCCTGGGCTTGAGCTCGACCAACTTCTC
TGAGCTCCAGAGCCAGATGCAGCAGCAGCTTATGGCCAGCCCTGAGATGA
TGATCCAAATAATGGAAAATCCCTTTGTTCAGAGCATGCTTTCGAATCCC
GATCTGATGAGGCAGCTCATTATGGCTAATCCACAGATGCAGCAATTGAT
TCAGAGAAACCCAGAAATCAGTCACCTGCTCAACAACCCAGACATAATGA
GGCAGACACTCGAAATTGCCAGGAATCCAGCCATGATGCAAGAGATGATG
AGAAATCAAGACTTAGCCTTATCTAACTTGGAAAGCATCCCAGGTGGCTA
TAATGCTTTACGGCGCATGTACACTGACATTCAAGAGCCGATGCTGAATG
CCGCACAAGAGCAGTTTGGGGGTAATCCATTTGCCTCCGTGGGGAGTAGT
TCCTCCTCTGGGGAAGGTACGCAGCCTTCCCGCACAGAAAATCGCGATCC
ACTACCCAATCCATGGGCACCACCGCCAGCTACCCAGAGTTCTGCAACTA
CCAGCACGACCACAAGCACTGGTAGTGGGTCTGGCAATAGTTCCAGCAAT
GCTACTGGGAACACCGTTGCTGCCGCTAATTATGTCGCCAGCATCTTTAG
TACCCCAGGCATGCAGAGCCTGCTGCAACAGATAACTGAAAACCCCCAGC
TGATTCAGAATATGCTGTCGGCGCCCTACATGAGAAGCATGATGCAGTCG
CTGAGCCAGAATCCAGATTTGGCTGCACAGATGATGCTGAATAGCCCGCT
GTTTACTGCAAATCCTCAGCTGCAGGAGCAGATGCGGCCACAGCTCCCAG
CCTTCCTGCAGCAGATGCAGAATCCAGACACACTATCAGCCATGTCAAAC
CCAAGAGCAATGCAGGCTTTAATGCAGATCCAGCAGGGGCTACAGACATT
AGCCACTGAAGCACCTGGCCTGATTCCGAGCTTCACTCCAGGTGTGGGGG
TGGGGGTGCTGGGAACCGCTATAGGCCCTGTAGGCCCAGTCACCCCCATA
GGCCCCATAGGCCCTATAGTCCCTTTTACCCCCATAGGCCCCATTGGGCC
CATAGGACCCACTGGCCCTGCAGCCCCCCCTGGCTCCACCGGCTCTGGTG
GCCCCACGGGGCCTACTGTGTCCAGCGCTGCACCTAGTGAAACCACGAGT
CCTACATCAGAATCTGGACCCAACCAGCAGTTCATTCAGCAAATGGTGCA
GGCCCTGGCTGGAGCAAATGCTCCACAGCTGCCGAATCCAGAAGTCAGAT
TTCAGCAACAACTGGAACAGCTCAACGCAATGGGGTTCTTAAACCGTGAA
GCAAACTTGCAGGCCCTAATAGCAACAGGAGGCGACATCAATGCAGCCAT
TGAAAGGCTGCTGGGCTCCCAGCCATCGTAAGCGGCCGC

Amino Acid Sequence

MAENGESSGPPRPSRGPAAAQGSAAAPAEPKIIKVTVKTPKEKEEFAVPE
NSSVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLIQHGIHDGLTVHL
VIKSQNRPQGQSTQPSNAAGTNTTSASTPRSNSTPISTNSNPFGLGSLGG
LAGLSSLGLSSTNFSELQSQMQQQLMASPEMMIQIMENPFVQSMLSNPDL
MRQLIMANPQMQQLIQRNPEISHLLNNPDIMRQTLEIARNPAMMQEMMRN
QDLALSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGGNPFASVGSSSS
SGEGTQPSRTENRDPLPNPWAPPPATQSSATTSTTTSTGSGSGNSSSNAT
GNTVAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSAPYMRSMMQSLS
QNPDLAAQMMLNSPLFTANPQLQEQMRPQLPAFLQQMQNPDTLSAMSNPR
AMQALMQIQQGLQTLATEAPGLIPSFTPGVGVGVLGTAIGPVGPVTPIGP
IGPIVPFTPIGPIGPIGPTGPAAPPGSTGSGGPTGPTVSSAAPSETTSPT
SESGPNQQFIQQMVQALAGANAPQLPNPEVRFQQQLEQLNAMGFLNREAN
LQALIATGGDINAAIERLLGSQPS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24105
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: siRNA resistant mutations:; 757-tta GCc tTa tct AAc tTg GaA-777; nt777 needs to be changed from A to g (NW2526/27 ordered)