cDNA Clone UBQLN2

• Synonyms: Ubiquilin-2
• Plasmid: pCMV Flag UBQLN2 Delta aa581-623 siRNA resistant (1)
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-UBQLN2 delta aa581-623
• Genbank: NM_013444.3
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGCTGAGAATGGCGAGAGCAGCGGCCCCCCGCGCCCCTCCCG
CGGCCCTGCTGCGGCCCAAGGCTCGGCTGCTGCCCCGGCTGAGCCTAAAA
TCATCAAAGTCACGGTGAAGACTCCCAAAGAGAAAGAGGAGTTCGCGGTG
CCCGAGAACAGCTCGGTTCAGCAGTTTAAGGAAGCGATTTCGAAACGCTT
CAAATCCCAAACCGATCAGCTAGTGCTGATTTTTGCCGGAAAAATCTTAA
AAGATCAAGATACCTTGATCCAGCATGGCATCCATGATGGGCTGACTGTT
CACCTTGTCATCAAAAGCCAGAACCGACCTCAGGGCCAGTCCACGCAGCC
TAGCAATGCCGCGGGAACTAACACTACCTCGGCGTCGACTCCCAGGAGTA
ACTCCACACCTATTTCCACAAATAGCAACCCGTTTGGGTTGGGGAGCCTG
GGAGGACTTGCAGGCCTTAGCAGCCTGGGCTTGAGCTCGACCAACTTCTC
TGAGCTCCAGAGCCAGATGCAGCAGCAGCTTATGGCCAGCCCTGAGATGA
TGATCCAAATAATGGAAAATCCCTTTGTTCAGAGCATGCTTTCGAATCCC
GATCTGATGAGGCAGCTCATTATGGCTAATCCACAGATGCAGCAATTGAT
TCAGAGAAACCCAGAAATCAGTCACCTGCTCAACAACCCAGACATAATGA
GGCAGACACTCGAAATTGCCAGGAATCCAGCCATGATGCAAGAGATGATG
AGAAATCAAGACCTGGCTCTTAGCAATCTAGAAAGCATCCCAGGTGGCTA
TAATGCTTTACGGCGCATGTACACTGACATTCAAGAGCCGATGCTGAATG
CCGCACAAGAGCAGTTTGGGGGTAATCCATTTGCCTCCGTGGGGAGTAGT
TCCTCCTCTGGGGAAGGTACGCAGCCTTCCCGCACAGAAAATCGCGATCC
ACTACCCAATCCATGGGCACCACCGCCAGCTACCCAGAGTTCTGCAACTA
CCAGCACGACCACAAGCACTGGTAGTGGGTCTGGCAATAGTTCCAGCAAT
GCTACTGGGAACACCGTTGCTGCCGCTAATTATGTCGCCAGCATCTTTAG
TACCCCAGGCATGCAGAGCCTGCTGCAACAGATAACTGAAAACCCCCAGC
TGATTCAGAATATGCTGTCGGCGCCCTACATGAGAAGCATGATGCAGTCG
CTGAGCCAGAATCCAGATTTGGCTGCACAGATGATGCTGAATAGCCCGCT
GTTTACTGCAAATCCTCAGCTGCAGGAGCAGATGCGGCCACAGCTCCCAG
CCTTCCTGCAGCAGATGCAGAATCCAGACACACTATCAGCCATGTCAAAC
CCAAGAGCAATGCAGGCTTTAATGCAGATCCAGCAGGGGCTACAGACATT
AGCCACTGAAGCACCTGGCCTGATTCCGAGCTTCACTCCAGGTGTGGGGG
TGGGGGTGCTGGGAACCGCTATAGGCCCTGTAGGCCCAGTCACCCCCATA
GGCCCCATAGGCCCTATAGTCCCTTTTACCCCCATAGGCCCCATTGGGCC
CATAGGACCCACTGGCCCTGCAGCCCCCCCTGGCTCCACCGGCTCTGGTG
GCCCCACGGGGCCTACTGTGTCCAGCGCTGCACCTAGTGAAACTACGTCA
CCAACGAGCGAATCTGGACCCAACCAGCAGTTCATTCAGCAAATGGTGCA
GGCCCTGGCTGGAGCAAATGCTCCACAGCTGCCGAATCCAGAAGTCTCGT
AAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMAENGESSGPPRPSRGPAAAQGSAAAPAEPKIIKVTVKT
PKEKEEFAVPENSSVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLIQ
HGIHDGLTVHLVIKSQNRPQGQSTQPSNAAGTNTTSASTPRSNSTPISTN
SNPFGLGSLGGLAGLSSLGLSSTNFSELQSQMQQQLMASPEMMIQIMENP
FVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHLLNNPDIMRQTLEIAR
NPAMMQEMMRNQDLALSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGG
NPFASVGSSSSSGEGTQPSRTENRDPLPNPWAPPPATQSSATTSTTTSTG
SGSGNSSSNATGNTVAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSA
PYMRSMMQSLSQNPDLAAQMMLNSPLFTANPQLQEQMRPQLPAFLQQMQN
PDTLSAMSNPRAMQALMQIQQGLQTLATEAPGLIPSFTPGVGVGVLGTAI
GPVGPVTPIGPIGPIVPFTPIGPIGPIGPTGPAAPPGSTGSGGPTGPTVS
SAAPSETTSPTSESGPNQQFIQQMVQALAGANAPQLPNPEVS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24083
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: WT sequence 1638-CACGAGTCCTACATCA-1653 mutated to 1638-tACgtcaCCaACgagc