cDNA Clone UBQLN2

• Plasmid: pCMV5-FLAG-1-UBQLN2 (delta 477-540) siRNA resistant (1)
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-UBQLN2 (delta 477-540)
• Genbank: Gene ID 29978
• Tag: FLAG
• Species: Human

Sequence of Insert

ATGGCTGAGAATGGCGAGAGCAGCGGCCCCCCGCGCCCCTCCCGCGGCCC
TGCTGCGGCCCAAGGCTCGGCTGCTGCCCCGGCTGAGCCTAAAATCATCA
AAGTCACGGTGAAGACTCCCAAAGAGAAAGAGGAGTTCGCGGTGCCCGAG
AACAGCTCGGTTCAGCAGTTTAAGGAAGCGATTTCGAAACGCTTCAAATC
CCAAACCGATCAGCTAGTGCTGATTTTTGCCGGAAAAATCTTAAAAGATC
AAGATACCTTGATCCAGCATGGCATCCATGATGGGCTGACTGTTCACCTT
GTCATCAAAAGCCAGAACCGACCTCAGGGCCAGTCCACGCAGCCTAGCAA
TGCCGCGGGAACTAACACTACCTCGGCGTCGACTCCCAGGAGTAACTCCA
CACCTATTTCCACAAATAGCAACCCGTTTGGGTTGGGGAGCCTGGGAGGA
CTTGCAGGCCTTAGCAGCCTGGGCTTGAGCTCGACCAACTTCTCTGAGCT
CCAGAGCCAGATGCAGCAGCAGCTTATGGCCAGCCCTGAGATGATGATCC
AAATAATGGAAAATCCCTTTGTTCAGAGCATGCTTTCGAATCCCGATCTG
ATGAGGCAGCTCATTATGGCTAATCCACAGATGCAGCAATTGATTCAGAG
AAACCCAGAAATCAGTCACCTGCTCAACAACCCAGACATAATGAGGCAGA
CACTCGAAATTGCCAGGAATCCAGCCATGATGCAAGAGATGATGAGAAAT
CAAGACCTGGCTCTTAGCAATCTAGAAAGCATCCCAGGTGGCTATAATGC
TTTACGGCGCATGTACACTGACATTCAAGAGCCGATGCTGAATGCCGCAC
AAGAGCAGTTTGGGGGTAATCCATTTGCCTCCGTGGGGAGTAGTTCCTCC
TCTGGGGAAGGTACGCAGCCTTCCCGCACAGAAAATCGCGATCCACTACC
CAATCCATGGGCACCACCGCCAGCTACCCAGAGTTCTGCAACTACCAGCA
CGACCACAAGCACTGGTAGTGGGTCTGGCAATAGTTCCAGCAATGCTACT
GGGAACACCGTTGCTGCCGCTAATTATGTCGCCAGCATCTTTAGTACCCC
AGGCATGCAGAGCCTGCTGCAACAGATAACTGAAAACCCCCAGCTGATTC
AGAATATGCTGTCGGCGCCCTACATGAGAAGCATGATGCAGTCGCTGAGC
CAGAATCCAGATTTGGCTGCACAGATGATGCTGAATAGCCCGCTGTTTAC
TGCAAATCCTCAGCTGCAGGAGCAGATGCGGCCACAGCTCCCAGCCTTCC
TGCAGCAGATGCAGAATCCAGACACACTATCAGCCATGTCAAACCCAAGA
GCAATGCAGGCTTTAATGCAGATCCAGCAGGGGCTACAGACATTAGCCAC
TGAAGCACCTGGCCTGATTCCGAGCTTCGCTGCACCTAGTGAAACTACGT
CACCAACGAGCGAATCTGGACCCAACCAGCAGTTCATTCAGCAAATGGTG
CAGGCCCTGGCTGGAGCAAATGCTCCACAGCTGCCGAATCCAGAAGTCAG
ATTTCAGCAACAACTGGAACAGCTCAACGCAATGGGGTTCTTAAACCGTG
AAGCAAACTTGCAGGCCCTAATAGCAACAGGAGGCGACATCAATGCAGCC
ATTGAAAGGCTGCTGGGCTCCCAGCCATCGTAA

Amino Acid Sequence

MDYKDDDDKGSMAENGESSGPPRPSRGPAAAQGSAAAPAEPKIIKVTVKT
PKEKEEFAVPENSSVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLIQ
HGIHDGLTVHLVIKSQNRPQGQSTQPSNAAGTNTTSASTPRSNSTPISTN
SNPFGLGSLGGLAGLSSLGLSSTNFSELQSQMQQQLMASPEMMIQIMENP
FVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHLLNNPDIMRQTLEIAR
NPAMMQEMMRNQDLALSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGG
NPFASVGSSSSSGEGTQPSRTENRDPLPNPWAPPPATQSSATTSTTTSTG
SGSGNSSSNATGNTVAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSA
PYMRSMMQSLSQNPDLAAQMMLNSPLFTANPQLQEQMRPQLPAFLQQMQN
PDTLSAMSNPRAMQALMQIQQGLQTLATEAPGLIPSFAAPSETTSPTSES
GPNQQFIQQMVQALAGANAPQLPNPEVRFQQQLEQLNAMGFLNREANLQA
LIATGGDINAAIERLLGSQPS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24082
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: WT sequence 1638-CACGAGTCCTACATCA-1653 mutated to 1638-tACgtcaCCaACgagc