cDNA Clone UBQLN2

• Plasmid: pCMV UBQLN2 P497H siRNA resistant (1)
• Parent Plasmid: pCMV5
• Expressed: UBQLN2 P497H
• Genbank: Gene ID 29978
• Species: Human

Sequence of Insert

ATGGCTGAGAATGGTGAGAGCAGCGGCCCCCCGCGCCCCTCCCGCGGCCC
TGCTGCGGCCCAAGGCTCGGCTGCTGCCCCGGCTGAGCCTAAAATCATCA
AAGTCACGGTGAAGACTCCCAAAGAGAAAGAGGAGTTCGCGGTGCCCGAG
AACAGCTCGGTTCAGCAGTTTAAGGAAGCGATTTCGAAACGCTTCAAATC
CCAAACCGATCAGCTAGTGCTGATTTTTGCCGGAAAAATCTTAAAAGATC
AAGATACCTTGATCCAGCATGGCATCCATGATGGGCTGACTGTTCACCTT
GTCATCAAAAGCCAGAACCGACCTCAGGGCCAGTCCACGCAGCCTAGCAA
TGCCGCGGGAACTAACACTACCTCGGCGTCGACTCCCAGGAGTAACTCCA
CACCTATTTCCACAAATAGCAACCCGTTTGGGTTGGGGAGCCTGGGAGGA
CTTGCAGGCCTTAGCAGCCTGGGCTTGAGCTCGACCAACTTCTCTGAGCT
CCAGAGCCAGATGCAGCAGCAGCTTATGGCCAGCCCTGAGATGATGATCC
AAATAATGGAAAATCCCTTTGTTCAGAGCATGCTTTCGAATCCCGATCTG
ATGAGGCAGCTCATTATGGCTAATCCACAGATGCAGCAATTGATTCAGAG
AAACCCAGAAATCAGTCACCTGCTCAACAACCCAGACATAATGAGGCAGA
CACTCGAAATTGCCAGGAATCCAGCCATGATGCAAGAGATGATGAGAAAT
CAAGACCTGGCTCTTAGCAATCTAGAAAGCATCCCAGGTGGCTATAATGC
TTTACGGCGCATGTACACTGACATTCAAGAGCCGATGCTGAATGCCGCAC
AAGAGCAGTTTGGGGGTAATCCATTTGCCTCCGTGGGGAGTAGTTCCTCC
TCTGGGGAAGGTACGCAGCCTTCCCGCACAGAAAATCGCGATCCACTACC
CAATCCATGGGCACCACCGCCAGCTACCCAGAGTTCTGCAACTACCAGCA
CGACCACAAGCACTGGTAGTGGGTCTGGCAATAGTTCCAGCAATGCTACT
GGGAACACCGTTGCTGCCGCTAATTATGTCGCCAGCATCTTTAGTACCCC
AGGCATGCAGAGCCTGCTGCAACAGATAACTGAAAACCCCCAGCTGATTC
AGAATATGCTGTCGGCGCCCTACATGAGAAGCATGATGCAGTCGCTGAGC
CAGAATCCAGATTTGGCTGCACAGATGATGCTGAATAGCCCGCTGTTTAC
TGCAAATCCTCAGCTGCAGGAGCAGATGCGGCCACAGCTCCCAGCCTTCC
TGCAGCAGATGCAGAATCCAGACACACTATCAGCCATGTCAAACCCAAGA
GCAATGCAGGCTTTAATGCAGATCCAGCAGGGGCTACAGACATTAGCCAC
TGAAGCACCTGGCCTGATTCCGAGCTTCACTCCAGGTGTGGGGGTGGGGG
TGCTGGGAACCGCTATAGGCCCTGTAGGCCCAGTCACCCATATAGGCCCC
ATAGGCCCTATAGTCCCTTTTACCCCCATAGGCCCCATTGGGCCCATAGG
ACCCACTGGCCCTGCAGCCCCCCCTGGCTCCACCGGCTCTGGTGGCCCCA
CGGGGCCTACTGTGTCCAGCGCTGCACCTAGTGAAACTACGTCACCAACG
AGCGAATCTGGACCCAACCAGCAGTTCATTCAGCAAATGGTGCAGGCCCT
GGCTGGAGCAAATGCTCCACAGCTGCCGAATCCAGAAGTCAGATTTCAGC
AACAACTGGAACAGCTCAACGCAATGGGGTTCTTAAACCGTGAAGCAAAC
TTGCAGGCCCTAATAGCAACAGGAGGCGACATCAATGCAGCCATTGAAAG
GCTGCTGGGCTCCCAGCCATCGTAA

Amino Acid Sequence

MAENGESSGPPRPSRGPAAAQGSAAAPAEPKIIKVTVKTPKEKEEFAVPE
NSSVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLIQHGIHDGLTVHL
VIKSQNRPQGQSTQPSNAAGTNTTSASTPRSNSTPISTNSNPFGLGSLGG
LAGLSSLGLSSTNFSELQSQMQQQLMASPEMMIQIMENPFVQSMLSNPDL
MRQLIMANPQMQQLIQRNPEISHLLNNPDIMRQTLEIARNPAMMQEMMRN
QDLALSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGGNPFASVGSSSS
SGEGTQPSRTENRDPLPNPWAPPPATQSSATTSTTTSTGSGSGNSSSNAT
GNTVAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSAPYMRSMMQSLS
QNPDLAAQMMLNSPLFTANPQLQEQMRPQLPAFLQQMQNPDTLSAMSNPR
AMQALMQIQQGLQTLATEAPGLIPSFTPGVGVGVLGTAIGPVGPVTHIGP
IGPIVPFTPIGPIGPIGPTGPAAPPGSTGSGGPTGPTVSSAAPSETTSPT
SESGPNQQFIQQMVQALAGANAPQLPNPEVRFQQQLEQLNAMGFLNREAN
LQALIATGGDINAAIERLLGSQPS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24081
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: WT sequence 1638-CACGAGTCCTACATCA-1653 mutated to 1638-tACgtcaCCaACgagc