cDNA Clone USP45

• Plasmid: pcDNA5frtTO GFP NLS USP45 C199A
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-NLS-USP45 C199A
• Genbank: NP_001073950.1
• Tag: GFP
• Species: Human

Sequence of Insert

AGATCTATGCGGGTGAAAGATCCAACTAAAGCTTTACCTGAGAAAGCCAAAAGAAGTAAAAGGCCTACTGTACCTCATGATGAAGACTCTTCAGATGATATTGCTGTAGGTTTAACTTGCCAACATGTAAGTCATGCTATCAGCGTGAATCATGTAAAGAGAGCAATAGCTGAGAATCTGTGGTCAGTTTGCTCAGAATGTTTAAAAGAAAGAAGATTCTATGATGGGCAGCTAGTACTTACTTCTGATATTTGGTTGTGCCTCAAGTGTGGCTTCCAGGGATGTGGTAAAAACTCAGAAAGCCAACATTCATTGAAGCACTTTAAGAGTTCCAGAACAGAGCCCCATTGTATTATAATTAATCTGAGCACATGGATTATATGGTGTTATGAATGTGATGAAAAATTATCAACGCATTGTAATAAGAAGGTTTTGGCTCAGATAGTTGATTTTCTCCAGAAACATGCTTCTAAAACACAAACAAGTGCATTTTCTAGAATCATGAAACTTTGTGAAGAAAAATGTGAAACAGATGAAATACAGAAGGGAGGAAAATGCAGAAATTTATCTGTAAGAGGAATTACAAATTTAGGAAATACTGCCTTTTTTAATGCAGTCATGCAGAACTTGGCACAGACTTATACTCTTACTGATCTGATGAATGAGATCAAAGAAAGTAGTACAAAACTCAAGATTTTTCCTTCCTCAGACTCTCAGCTGGACCCATTGGTGGTGGAACTTTCAAGGCCTGGACCACTGACTTCTGCCTTGTTCCTGTTTCTTCACAGCATGAAGGAGACTGAAAAAGGACCACTTTCTCCTAAAGTTCTTTTTAATCAGCTTTGTCAGAAGGCACCTCGATTTAAAGATTTCCAGCAACAGGACAGTCAGGAGCTTCTTCATTATCTTCTGGATGCAGTGAGGACAGAAGAAACAAAGCGAATACAAGCTAGCATTCTAAAAGCATTTAACAACCCAACTACTAAAACTGCTGATGATGAAACTAGAAAAAAAGTCAAAGCATATGGAAAAGAAGGTGTGAAAATGAACTTCATAGATCGGATCTTTATTGGTGAATTAACTAGCACGGTCATGTGTGAAGAATGTGCAAATATCTCCACGGTGAAAGATCCATTCATTGATATTTCACTTCCTATAATAGAAGAAAGGGTTTCAAAACCTTTACTTTGGGGAAGAATGAATAAATATAGAAGTTTACGGGAGACAGATCATGATCGATACAGTGGCAATGTTACTATAGAAAATATTCATCAACCTAGAGCTGCCAAGAAGCATTCTTCATCTAAAGATAAGAGTCAACTAATTCATGACCGAAAATGTATTAGAAAATTGTCATCTGGAGAAACTGTCACATACCAGAAAAATGAAAACCTTGAAATGAATGGGGATTCTTTAATGTTTGCCAGCCTCATGAATTCTGAGTCACGTCTGAATGAAAGCCCTACTGATGACAGTGAAAAAGAAGCCAGCCATTCTGAAAGCAATGTTGATGCTGACAGTGAGCCTTCAGAATCTGAAAGTGCTTCAAAGCAGACTGGGCTGTTCAGATCCAGTAGTGGATCCGGTGTGCAGCCAGATGGACCCCTTTACCCTCTGTCAGCAGGTAAACTGCTGTACACCAAGGAGACTGACAGTGGTGATAAGGAAATGGCAGAAGCTATTTCTGAACTTCGTTTGAGCAGCACTGTAACTGGAGATCAAGATTTTGACAGAGAAAATCAGCCACTAAATATTTCAAATAATTTATGTTTTTTGGAGGGGAAGCATTTGAGGTCTTATAGTCCCCAAAATGCTTTTCAGACCCTTTCTCAGAGCTATATAACTACTTCTAAAGAATGTTCAATTCAGTCCTGTCTCTACCAGTTTACATCTATGGAATTACTAATGGGGAATAATAAGCTTCTATGTGAGAATTGTACTAAAAACAAACAGAAGTACCAAGAAGAAACCAGTTTTGCAGAAAAGAAAGTAGAAGGAGTTTATACTAATGCCAGGAAGCAATTGCTCATTTCTGCTGTTCCAGCTGTCCTAATTCTCCACCTGAAAAGATTTCATCAGGCTGGCTTGAGTCTTCGTAAAGTAAACAGACATGTAGATTTTCCACTTATGCTCGATTTAGCACCATTCTGCTCTGCTACTTGTAAGAATGCAAGTGTGGGAGATAAAGTTCTCTACGGTCTCTATGGCATAGTGGAACATAGTGGCTCGATGAGAGAAGGCCACTACACTGCTTATGTGAAAGTGAGAACACCCTCCAGGAAATTATCGGAACATAACACTAAAAAGAAAAATGTGCCTGGTTTGAAAGCGGCTGATAATGAATCAGCAGGCCAGTGGGTCCATGTTAGTGACACTTACTTACAGGTGGTTCCAGAATCAAGAGCACTTAGTGCACAAGCCTACCTTCTTTTCTATGAAAGAGTATTATAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMRVKDP
TKALPEKAKRSKRPTVPHDEDSSDDIAVGLTCQHVSHAISVNHVKRAIAE
NLWSVCSECLKERRFYDGQLVLTSDIWLCLKCGFQGCGKNSESQHSLKHF
KSSRTEPHCIIINLSTWIIWCYECDEKLSTHCNKKVLAQIVDFLQKHASK
TQTSAFSRIMKLCEEKCETDEIQKGGKCRNLSVRGITNLGNTAFFNAVMQ
NLAQTYTLTDLMNEIKESSTKLKIFPSSDSQLDPLVVELSRPGPLTSALF
LFLHSMKETEKGPLSPKVLFNQLCQKAPRFKDFQQQDSQELLHYLLDAVR
TEETKRIQASILKAFNNPTTKTADDETRKKVKAYGKEGVKMNFIDRIFIG
ELTSTVMCEECANISTVKDPFIDISLPIIEERVSKPLLWGRMNKYRSLRE
TDHDRYSGNVTIENIHQPRAAKKHSSSKDKSQLIHDRKCIRKLSSGETVT
YQKNENLEMNGDSLMFASLMNSESRLNESPTDDSEKEASHSESNVDADSE
PSESESASKQTGLFRSSSGSGVQPDGPLYPLSAGKLLYTKETDSGDKEMA
EAISELRLSSTVTGDQDFDRENQPLNISNNLCFLEGKHLRSYSPQNAFQT
LSQSYITTSKECSIQSCLYQFTSMELLMGNNKLLCENCTKNKQKYQEETS
FAEKKVEGVYTNARKQLLISAVPAVLILHLKRFHQAGLSLRKVNRHVDFP
LMLDLAPFCSATCKNASVGDKVLYGLYGIVEHSGSMREGHYTAYVKVRTP
SRKLSEHNTKKKNVPGLKAADNESAGQWVHVSDTYLQVVPESRALSAQAY
LLFYERVL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44723
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C199A. Contains an n-ter nuclear localisation signal.