cDNA Clone TTBK2

• Synonyms: KIAA0847, SCA11, TTBK
• Plasmid: pGEX6P-1 TTBK2 D163A
• Parent Plasmid: pGEX6P1
• Expressed: GST-TTBK2 D163A
• Genbank: NM_173500.3
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGAGTGGGGGAGGAGAGCAGCCGGATATCCTGAGTGTTGG
AATCCTAGTGAAAGAAAGATGGAAAGTGTTGAGAAAGATTGGGGGTGGGG
GCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAAAATGTT
GCACTGAAGGTGGAATCAGCTCAACAACCAAAACAAGTTCTGAAAATGGA
AGTTGCTGTTTTGAAAAAGCTGCAAGGGAAAGACCATGTTTGTAGATTTA
TTGGCTGTGGGAGGAATGATCGATTCAACTATGTGGTCATGCAGTTGCAG
GGTCGGAATCTGGCAGATCTTCGCCGTAGCCAGTCCCGAGGCACATTCAC
CATTAGTACCACTCTCCGGCTGGGTAGACAGATTTTGGAGTCTATTGAAA
GCATTCATTCTGTGGGATTCTTGCATCGAGACATCAAACCGTCGAACTTC
GCTATGGGTCGCTTTCCTAGTACATGTAGGAAATGTTACATGCTTGcTTT
TGGCTTGGCTCGACAATTTACCAATTCCTGTGGTGACGTCAGACCACCTC
GAGCTGTGGCAGGTTTTCGAGGGACAGTTCGTTATGCATCAATCAACGCA
CATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGGTCCTTATTCTA
CATGTTGGTGGAGTTTGTGGTTGGTCAGCTGCCCTGGAGAAAAATAAAGG
ACAAGGAGCAAGTAGGCTCTATTAAGGAGAGATATGACCACAGGCTCATG
TTGAAACATCTCCCTCCAGAATTCAGCATCTTTCTAGACCATATCTCTTC
TTTGGATTATTTTACAAAACCAGACTACCAGCTTCTTACATCCGTGTTTG
ACAATAGCATCAAGACTTTTGGAGTAATTGAGAGTGACCCTTTTGACTGG
GAGAAGACTGGAAATGATGGCTCCCTAACAACCACCACTACTTCTACCAC
CCCTCAGTTGCACACTCGCTTGACCCCTGCTGCAATTGGAATTGCCAATG
CTACTCCCATCCCTGGAGACTTGCTTCGAGAAAATACAGATGAGGTATTT
CCAGATGAACAGCTTAGCGATGGAGAAAATGGCATCCCTGTTGGTGTGTC
ACCAGATAAATTGCCTGGATCTCTGGGACACCCCCGTCCCCAGGAGAAGG
ATGTTTGGGAAGAGATGGATGCCAACAAAAACAAGATAAAGCTTGGAATT
TGTAAGGCTGCTACTGAAGAGGAGAACAGCCATGGCCAGGCAAATGGTCT
TCTCAATGCTCCAAGCCTTGGGTCACCAATTCGTGTCCGCTCAGAGATTA
CTCAGCCAGACAGAGATATTCCACTGGTGCGAAAGTTACGTTCCATTCAC
AGCTTTGAGCTGGAAAAACGTCTGACCCTGGAGCCAAAGCCAGACACTGA
CAAGTTCCTTGAGACCTGCCTGGAGAAAATGCAGAAAGATACCAGTGCAG
GAAAAGAATCTATTCTCCCTGCTCTGCTGCATAAGCCTTGCGTTCCTGCT
GTGTCCCGTACTGACCACATCTGGCACTATGATGAAGAATATCTTCCAGA
TGCCTCCAAGCCTGCTTCTGCCAACACCCCTGAGCAGGCAGATGGTGGTG
GCAGCAATGGATTTATAGCTGTTAACCTGAGCTCTTGCAAGCAAGAAATT
GATTCCAAAGAATGGGTGATTGTGGACAAGGAGCAGGACCTTCAGGATTT
TAGGACAAATGAGGCTGTAGGACATAAAACAACTGGAAGTCCTTCTGATG
AGGAGCCTGAAGTACTTCAAGTCCTGGAGGCATCACCTCAAGATGAAAAG
CTCCAGTTAGGTCCTTGGGCAGAAAATGATCATTTAAAGAAGGAAACCTC
AGGTGTGGTCTTAGCACTTTCTGCAGAGGGTCCTCCTACTGCTGCTTCAG
AACAATATACAGATAGGCTGGAACTCCAGCCTGGAGCTGCTAGTCAGTTT
ATTGCAGCGACGCCCACAAGTCTAATGGAGGCGCAGGCAGAAGGACCCCT
TACAGCGATTACAATTCCTAGACCTTCTGTGGCATCTACACAGTCAACTT
CAGGAAGCTTTCACTGTGGTCAGCAGCCAGAGAAGAAAGATCTTCAGCCC
ATGGAGCCCACTGTGGAACTTTACTCTCCAAGGGAAAACTTCTCTGGCTT
GGTTGTGACAGAGGGTGAACCTCCTAGTGGAGGAAGCAGAACAGATTTGG
GGCTTCAGATAGATCACATTGGTCATGACATGTTACCCAACATTAGAGAA
AGTAACAAATCTCAAGACCTGGGACCAAAAGAACTTCCTGATCATAATAG
ACTGGTTGTGAGAGAATTTGAAAATCTCCCTGGGGAAACTGAAGAGAAAA
GCATCCTTTTAGAGTCAGATAATGAAGATGAGAAGTTAAGTAGAGGGCAG
CATTGTATTGAGATCTCCTCTCTCCCAGGAGATTTGGTAATTGTGGAAAA
GGATCACTCAGCTACTACTGAACCTCTTGATGTGACAAAAACACAGACTT
TTAGTGTGGTGCCAAATCAAGACAAAAATAATGAGATAATGAAGCTTCTG
ACAGTTGGAACTTCAGAAATTTCTTCCAGAGACATTGACCCACATGTTGA
AGGTCAGATAGGCCAAGTGGCAGAAATGCAAAAAAATAAGATATCTAAGG
ATGATGACATCATGAGTGAAGACTTGCCAGGTCATCAAGGAGACCTCTCT
ACTTTTTTGCACCAAGAGGGCAAGAGAGAGAAAATCACCCCTAGAAATGG
AGAACTATTTCATTGTGTTTCAGAGAATGAACATGGTGCCCCAACCCGGA
AGGATATGGTTAGGTCATCCTTTGTAACTAGACACAGCCGAATCCCTGTT
TTAGCACAAGAGATAGACTCAACTTTGGAATCATCCTCTCCAGTTTCTGC
AAAAGAAAAGCTCCTCCAAAAGAAAGCCTATCAGCCAGACCTAGTCAAGC
TTCTGGTGGAAAAAAGACAATTCAAGTCCTTCCTTGGCGACCTCTCAAGT
GCCTCTGATAAATTGCTAGAGGAGAAACTAGCTACTGTTCCTGCTCCCTT
TTGTGAGGAGGAAGTGCTCACTCCCTTTTCAAGACTGACAGTAGATTCTC
ACCTGAGTAGGTCAGCTGAAGATAGCTTTCTGTCACCCATCATCTCCCAG
TCTAGAAAGAGCAAAATTCCAAGGCCAGTTTCATGGGTCAACACAGATCA
GGTCAATAGCTCAACTTCGTCTCAGTTCTTTCCTCGGCCACCACCAGGAA
AGCCACCCACGAGGCCTGGAGTAGAAGCCAGGCTACGCAGATATAAAGTC
CTAGGGAGTAGTAACTCCGACTCAGACCTTTTCTCCCGCCTGGCCCAAAT
TCTTCAAAATGGATCTCAGAAACCCCGGAGCACTACTCAGTGCAAGAGTC
CAGGATCTCCTCACAATCCAAAAACACCACCCAAGAGTCCAGTTGTCCCT
CGCAGGAGTCCCAGTGCCTCTCCTCGAAGCTCATCCTTGCCTCGCACGTC
TAGTTCCTCACCATCTAGGGCTGGACGGCCCCACCATGACCAGAGGAGTT
CGTCCCCACATCTGGGGAGAAGCAAGTCACCTCCCAGCCACTCAGGATCT
TCCTCCTCCAGGAGGTCCTGCCAACAGGAGCATTGCAAACCCAGCAAGAA
TGGCCTGAAAGGATCCGGCAGCCTCCACCACCACTCAGCCAGCACTAAAA
CCCCCCAAGGGAAGAGTAAGCCAGCCAGTAAACTCAGCAGATAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEIPGSTRAAAMSGGGEQP
DILSVGILVKERWKVLRKIGGGGFGEIYDALDMLTRENVALKVESAQQPK
QVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVVMQLQGRNLADLRRSQ
SRGTFTISTTLRLGRQILESIESIHSVGFLHRDIKPSNFAMGRFPSTCRK
CYMLAFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGRHDD
LWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERYDHRLMLKHLPPEFSIF
LDHISSLDYFTKPDYQLLTSVFDNSIKTFGVIESDPFDWEKTGNDGSLTT
TTTSTTPQLHTRLTPAAIGIANATPIPGDLLRENTDEVFPDEQLSDGENG
IPVGVSPDKLPGSLGHPRPQEKDVWEEMDANKNKIKLGICKAATEEENSH
GQANGLLNAPSLGSPIRVRSEITQPDRDIPLVRKLRSIHSFELEKRLTLE
PKPDTDKFLETCLEKMQKDTSAGKESILPALLHKPCVPAVSRTDHIWHYD
EEYLPDASKPASANTPEQADGGGSNGFIAVNLSSCKQEIDSKEWVIVDKE
QDLQDFRTNEAVGHKTTGSPSDEEPEVLQVLEASPQDEKLQLGPWAENDH
LKKETSGVVLALSAEGPPTAASEQYTDRLELQPGAASQFIAATPTSLMEA
QAEGPLTAITIPRPSVASTQSTSGSFHCGQQPEKKDLQPMEPTVELYSPR
ENFSGLVVTEGEPPSGGSRTDLGLQIDHIGHDMLPNIRESNKSQDLGPKE
LPDHNRLVVREFENLPGETEEKSILLESDNEDEKLSRGQHCIEISSLPGD
LVIVEKDHSATTEPLDVTKTQTFSVVPNQDKNNEIMKLLTVGTSEISSRD
IDPHVEGQIGQVAEMQKNKISKDDDIMSEDLPGHQGDLSTFLHQEGKREK
ITPRNGELFHCVSENEHGAPTRKDMVRSSFVTRHSRIPVLAQEIDSTLES
SSPVSAKEKLLQKKAYQPDLVKLLVEKRQFKSFLGDLSSASDKLLEEKLA
TVPAPFCEEEVLTPFSRLTVDSHLSRSAEDSFLSPIISQSRKSKIPRPVS
WVNTDQVNSSTSSQFFPRPPPGKPPTRPGVEARLRRYKVLGSSNSDSDLF
SRLAQILQNGSQKPRSTTQCKSPGSPHNPKTPPKSPVVPRRSPSASPRSS
SLPRTSSSSPSRAGRPHHDQRSSSPHLGRSKSPPSHSGSSSSRRSCQQEH
CKPSKNGLKGSGSLHHHSASTKTPQGKSKPASKLSR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44589
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: D163A