cDNA Clone Cullin3

• Plasmid: pFBDM-Dac-TEV-Cullin3/FLAG-KLHL15
• Parent Plasmid: pFBDM
• Expressed: Dac-TEV-Cullin3
• Tag: DAC
• Species: Human

Sequence of Insert

ATGTCTAACCTGTCTAAGGGCACTGGCAGCCGTAAGGATACCAAGATGAGAATCAGAGCCTTCCCGATGACTATGGACGAAAAGTACGTGAACTCCATCTGGGACCTGCTCAAGAACGCTATCCAGGAGATCCAAAGGAAGAACAACTCCGGCTTGTCTTTCGAGGAACTGTACAGAAACGCCTACACTATGGTGCTGCACAAGCACGGCGAGAAGCTCTACACTGGCTTGCGTGAAGTGGTCACAGAGCACCTCATCAACAAGGTTCGCGAAGATGTGCTCAACTCTTTGAACAACAACTTCCTGCAGACTCTCAACCAGGCTTGGAACGACCACCAGACAGCTATGGTCATGATCAGGGATATCCTGATGTACATGGACAGAGTCTACGTTCAGCAAAACAACGTGGAGAACGTCTACAACCTCGGATTGATCATCTTCCGTGACCAGGTTGTGCGCTACGGTTGCATCCGTGATCACCTCCGCCAAACCTTGCTGGACATGATCGCTCGTGAACGCAAGGGAGAGGTCGTTGATAGGGGTGCTATCAGAAACGCCTGTCAGATGCTGATGATCCTGGGTCTCGAAGGCAGGAGCGTCTACGAGGAAGACTTCGAGGCTCCCTTCCTCGAAATGTCGGCCGAGTTCTTCCAGATGGAATCCCAAAAGTTCTTGGCTGAGAACAGCGCCTCAGTCTACATCAAGAAGGTTGAAGCCCGTATCAACGAGGAAATCGAGCGCGTTATGCACTGCCTGGACAAGTCAACCGAGGAACCTATCGTCAAGGTGGTCGAGCGCGAACTCATCTCCAAGCACATGAAGACCATCGTCGAGATGGAAAACTCTGGTCTGGTTCACATGCTCAAGAACGGCAAGACTGAGGACCTGGGATGTATGTACAAGCTCTTCTCCAGGGTGCCCAACGGCCTGAAGACTATGTGCGAATGTATGTCCTCTTACCTCAGAGAGCAGGGAAAGGCTTTGGTTTCTGAGGAAGGCGAGGGAAAGAACCCTGTGGACTACATCCAAGGCCTCTTGGATTTGAAGTCTAGGTTCGACCGTTTCCTGCTGGAGTCCTTCAACAACGATAGGCTGTTCAAGCAGACCATCGCCGGAGACTTCGAGTACTTCTTGAACCTGAACTCCCGTTCCCCTGAATACCTCAGCTTGTTCATCGACGATAAGCTGAAGAAGGGAGTGAAGGGTCTCACCGAGCAAGAAGTCGAGACTATCTTGGATAAGGCTATGGTCCTGTTCCGTTTCATGCAGGAAAAGGACGTTTTCGAGCGCTACTACAAGCAACACCTGGCCCGTCGCTTGCTGACTAACAAGTCTGTGAGCGACGATAGCGAAAAGAACATGATCTCAAAGTTGAAGACCGAGTGCGGTTGTCAGTTCACTTCTAAGCTGGAAGGCATGTTCCGTGATATGTCAATCTCGAACACCACTATGGACGAGTTCCGCCAGCACCTGCAAGCTACCGGTGTGTCACTCGGTGGCGTGGACCTGACTGTGCGTGTCCTGACAACCGGCTACTGGCCCACTCAGTCGGCTACACCTAAGTGCAACATCCCCCCTGCTCCACGTCACGCCTTCGAAATCTTCAGGAGATTCTACTTGGCCAAGCACAGCGGACGCCAGCTGACACTCCAACACCACATGGGTTCAGCTGATCTGAACGCCACCTTCTACGGCCCGGTGAAGAAGGAAGACGGCTCCGAGGTTGGAGTGGGAGGTGCTCAGGTGACCGGTTCAAACACTCGCAAGCACATCCTGCAGGTCTCGACATTCCAAATGACCATCTTGATGCTGTTCAACAACAGGGAGAAGTACACATTCGAGGAAATCCAGCAAGAAACCGACATCCCAGAACGTGAGCTGGTGCGTGCTCTGCAGTCGCTGGCTTGCGGAAAGCCAACTCAACGCGTCTTGACAAAGGAGCCGAAGTCCAAGGAAATCGAGAACGGTCACATCTTCACAGTGAACGATCAGTTCACCTCTAAGCTGCACCGTGTTAAGATCCAGACTGTGGCTGCCAAGCAAGGCGAGAGCGACCCCGAAAGGAAGGAGACAAGACAGAAGGTGGACGATGACCGCAAGCACGAAATCGAGGCTGCCATCGTCAGGATCATGAAGAGCAGAAAGAAGATGCAACACAACGTCCTCGTTGCTGAGGTCACCCAGCAACTCAAGGCCAGGTTCTTGCCATCACCGGTTGTGATCAAGAAGAGAATCGAGGGTCTGATCGAGAGAGAATACTTGGCTAGGACTCCAGAGGACAGAAAGGTTTACACTTACGTTGCTTAA

Amino Acid Sequence

MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNPENLYFQGGSMSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU42657
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: EcoRI/NotI cuts out DacTEVCullin3, BamHI/NotI cuts out just Cullin3