cDNA Clone TTBK2

• Synonyms: KIAA0847, SCA11, TTBK
• Plasmid: pET15b TEV HALO TTBK2 331-930
• Parent Plasmid: pET15b 6His TEV Halo 3C
• Expressed: 6xHIS-HALO-TTBK2 331-930
• Genbank: NM_173500.3
• Tag: 6His
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCGCCATGGCTACTCCCATCCCTGGAGACTTGCTTCGAGAAAATAC
AGATGAGGTATTTCCAGATGAACAGCTTAGCGATGGAGAAAATGGCATCC
CTGTTGGTGTGTCACCAGATAAATTGCCTGGATCTCTGGGACACCCCCGT
CCCCAGGAGAAGGATGTTTGGGAAGAGATGGATGCCAACAAAAACAAGAT
AAAGCTTGGAATTTGTAAGGCTGCTACTGAAGAGGAGAACAGCCATGGCC
AGGCAAATGGTCTTCTCAATGCTCCAAGCCTTGGGTCACCAATTCGTGTC
CGCTCAGAGATTACTCAGCCAGACAGAGATATTCCACTGGTGCGAAAGTT
ACGTTCCATTCACAGCTTTGAGCTGGAAAAACGTCTGACCCTGGAGCCAA
AGCCAGACACTGACAAGTTCCTTGAGACCTGCCTGGAGAAAATGCAGAAA
GATACCAGTGCAGGAAAAGAATCTATTCTCCCTGCTCTGCTGCATAAGCC
TTGCGTTCCTGCTGTGTCCCGTACTGACCACATCTGGCACTATGATGAAG
AATATCTTCCAGATGCCTCCAAGCCTGCTTCTGCCAACACCCCTGAGCAG
GCAGATGGTGGTGGCAGCAATGGATTTATAGCTGTTAACCTGAGCTCTTG
CAAGCAAGAAATTGATTCCAAAGAATGGGTGATTGTGGACAAGGAGCAGG
ACCTTCAGGATTTTAGGACAAATGAGGCTGTAGGACATAAAACAACTGGA
AGTCCTTCTGATGAGGAGCCTGAAGTACTTCAAGTCCTGGAGGCATCACC
TCAAGATGAAAAGCTCCAGTTAGGTCCTTGGGCAGAAAATGATCATTTAA
AGAAGGAAACCTCAGGTGTGGTCTTAGCACTTTCTGCAGAGGGTCCTCCT
ACTGCTGCTTCAGAACAATATACAGATAGGCTGGAACTCCAGCCTGGAGC
TGCTAGTCAGTTTATTGCAGCGACGCCCACAAGTCTAATGGAGGCGCAGG
CAGAAGGACCCCTTACAGCGATTACAATTCCTAGACCTTCTGTGGCATCT
ACACAGTCAACTTCAGGAAGCTTTCACTGTGGTCAGCAGCCAGAGAAGAA
AGATCTTCAGCCCATGGAGCCCACTGTGGAACTTTACTCTCCAAGGGAAA
ACTTCTCTGGCTTGGTTGTGACAGAGGGTGAACCTCCTAGTGGAGGAAGC
AGAACAGATTTGGGGCTTCAGATAGATCACATTGGTCATGACATGTTACC
CAACATTAGAGAAAGTAACAAATCTCAAGACCTGGGACCAAAAGAACTTC
CTGATCATAATAGACTGGTTGTGAGAGAATTTGAAAATCTCCCTGGGGAA
ACTGAAGAGAAAAGCATCCTTTTAGAGTCAGATAATGAAGATGAGAAGTT
AAGTAGAGGGCAGCATTGTATTGAGATCTCCTCTCTCCCAGGAGATTTGG
TAATTGTGGAAAAGGATCACTCAGCTACTACTGAACCTCTTGATGTGACA
AAAACACAGACTTTTAGTGTGGTGCCAAATCAAGACAAAAATAATGAGAT
AATGAAGCTTCTGACAGTTGGAACTTCAGAAATTTCTTCCAGAGACATTG
ACCCACATGTTGAAGGTCAGATAGGCCAAGTGGCAGAAATGCAAAAAAAT
AAGATATCTAAGGATGATGACATCATGAGTGAAGACTTGCCAGGTCATCA
AGGAGACCTCTCTACTTTTTTGCACCAAGAGGGCAAGAGAGAGAAAATCA
CCCCTAGAAATGGAGAACTATTTCATTGTGTTTCAGAGAATGAACATGGT
GCCCCAACCCGGTAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSENLYFQGMAEIGTGFPFDPHYVEVLGE
RMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMG
KSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNP
ERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIE
GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVA
LVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPG
LNLLQEDNPDLIGSEIARWLSTLEISGLEVLFQGPGSAMATPIPGDLLRE
NTDEVFPDEQLSDGENGIPVGVSPDKLPGSLGHPRPQEKDVWEEMDANKN
KIKLGICKAATEEENSHGQANGLLNAPSLGSPIRVRSEITQPDRDIPLVR
KLRSIHSFELEKRLTLEPKPDTDKFLETCLEKMQKDTSAGKESILPALLH
KPCVPAVSRTDHIWHYDEEYLPDASKPASANTPEQADGGGSNGFIAVNLS
SCKQEIDSKEWVIVDKEQDLQDFRTNEAVGHKTTGSPSDEEPEVLQVLEA
SPQDEKLQLGPWAENDHLKKETSGVVLALSAEGPPTAASEQYTDRLELQP
GAASQFIAATPTSLMEAQAEGPLTAITIPRPSVASTQSTSGSFHCGQQPE
KKDLQPMEPTVELYSPRENFSGLVVTEGEPPSGGSRTDLGLQIDHIGHDM
LPNIRESNKSQDLGPKELPDHNRLVVREFENLPGETEEKSILLESDNEDE
KLSRGQHCIEISSLPGDLVIVEKDHSATTEPLDVTKTQTFSVVPNQDKNN
EIMKLLTVGTSEISSRDIDPHVEGQIGQVAEMQKNKISKDDDIMSEDLPG
HQGDLSTFLHQEGKREKITPRNGELFHCVSENEHGAPTR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44507
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 331-930. Use TEV to cleave off 6xHIS, precission may be used downstream to remove the HALO tag if necessary.