cDNA Clone UHRF1

• Synonyms: Np95, hNP95, ICBP90, RNF106, FLJ21925, MGC138707, ubiquitin-like with PHD and ring finger domains 1
• Plasmid: pFBDM-UHRF1; 6HIS-DNMT1
• Parent Plasmid: pFBDM
• Expressed: UHRF1
• Genbank: NM_001048201.1
• Tag: 6His
• Species: Human

Sequence of Insert

GTCGACggATGTGGATCCAGGTTCGGACCATGGATGGGAGGCAGACCCACACGGTGGACTCGCTGTCCAGGCTGACCAAGGTGGAGGAGCTGAGGCGGAAGATCCAGGAGCTGTTCCACGTGGAGCCAGGCCTGCAGAGGCTGTTCTACAGGGGCAAACAGATGGAGGACGGCCATACCCTCTTCGACTACGAGGTCCGCCTGAATGACACCATCCAGCTCCTGGTCCGCCAGAGCCTCGTGCTCCCCCACAGCACCAAGGAGCGGGACTCCGAGCTCTCCGACACCGACTCCGGCTGCTGCCTGGGCCAGAGTGAGTCAGACAAGTCCTCCACCCACGGTGAGGCGGCAGCCGAGACTGACAGCAGGCCAGCCGATGAGGACATGTGGGATGAGACGGAATTGGGGCTGTACAAGGTCAATGAGTACGTCGATGCTCGGGACACGAACATGGGGGCGTGGTTTGAGGCGCAGGTGGTCAGGGTGACGCGGAAGGCCCCCTCCCGGGACGAGCCCTGCAGCTCCACGTCCAGGCCGGCGCTGGAGGAGGACGTCATTTACCACGTGAAATACGACGACTACCCGGAGAACGGCGTGGTCCAGATGAACTCCAGGGACGTCCGAGCGCGCGCCCGCACCATCATCAAGTGGCAGGACCTGGAGGTGGGCCAGGTGGTCATGCTCAACTACAACCCCGACAACCCCAAGGAGCGGGGCTTCTGGTACGACGCGGAGATCTCCAGGAAGCGCGAGACCAGGACGGCGCGGGAACTCTACGCCAACGTGGTGCTGGGGGATGATTCTCTGAACGACTGTCGGATCATCTTCGTGGACGAAGTCTTCAAGATTGAGCGGCCGGGTGAAGGGAGCCCCATGGTTGACAACCCCATGAGACGGAAGAGCGGGCCGTCCTGCAAGCACTGCAAGGACGACGTGAACAGACTCTGCCGGGTCTGCGCCTGCCACCTGTGCGGGGGCCGGCAGGACCCCGACAAGCAGCTCATGTGCGATGAGTGCGACATGGCCTTCCACATCTACTGCCTGGACCCGCCCCTCAGCAGTGTTCCCAGCGAGGACGAGTGGTACTGCCCTGAGTGCCGGAATGATGCCAGCGAGGTGGTACTGGCGGGAGAGCGGCTGAGAGAGAGCAAGAAGAAGGCGAAGATGGCCTCGGCCACATCGTCCTCACAGCGGGACTGGGGCAAGGGCATGGCCTGTGTGGGCCGCACCAAGGAATGTACCATCGTCCCGTCCAACCACTACGGACCCATCCCGGGGATCCCCGTGGGCACCATGTGGCGGTTCCGAGTCCAGGTCAGCGAGTCGGGTGTCCATCGGCCCCACGTGGCTGGCATACACGGCCGGAGCAACGACGGAGCGTACTCCCTAGTCCTGGCGGGGGGCTATGAGGATGACGTGGACCATGGGAATTTTTTCACATACACGGGTAGTGGTGGTCGAGATCTTTCCGGCAACAAGAGGACCGCGGAACAGTCTTGTGATCAGAAACTCACCAACACCAACAGGGCGCTGGCTCTCAACTGCTTTGCTCCCATCAATGACCAAGAAGGGGCCGAGGCCAAGGACTGGCGGTCGGGGAAGCCGGTCAGGGTGGTGCGCAATGTCAAGGGTGGCAAGAATAGCAAGTACGCCCCCGCTGAGGGCAACCGCTATGATGGCATCTACAAGGTTGTGAAATACTGGCCCGAGAAGGGGAAGTCCGGGTTTCTCGTGTGGCGCTACCTTCTGCGGAGGGACGATGATGAGCCTGGCCCTTGGACGAAGGAGGGGAAGGACCGGATCAAGAAGCTGGGGCTGACCATGCAGTATCCAGAAGGCTACCTGGAAGCCCTGGCCAACCGAGAGCGAGAGAAGGAGAACAGCAAGAGGGAGGAGGAGGAGCAGCAGGAGGGGGGCTTCGCGTCCCCCAGGACGGGCAAGGGCAAGTGGAAGCGGAAGTCGGCAGGAGGTGGCCCGAGCAGGGCCGGGTCCCCGCGCCGGACATCCAAGAAAACCAAGGTGGAGCCCTACAGTCTCACGGCCCAGCAGAGCAGCCTCATCAGAGAGGACAAGAGCAACGCCAAGCTGTGGAATGAGGTCCTGGCGTCACTCAAGGACCGGCCGGCGAGCGGCAGCCCGTTCCAGTTGTTCCTGAGTAAAGTGGAGGAGACGTTCCAGTGTATCTGCTGTCAGGAGCTGGTGTTCCGGCCCATCACGACCGTGTGCCAGCACAACGTGTGCAAGGACTGCCTGGACAGATCCTTTCGGGCACAGGTGTTCAGCTGCCCTGCCTGCCGCTACGACCTGGGCCGCAGCTATGCCATGCAGGTGAACCAGCCTCTGCAGACCGTCCTCAACCAGCTCTTCCCCGGCTACGGCAATGGCCGGTGAgaattc

Amino Acid Sequence

MWIQVRTMDGRQTHTVDSLSRLTKVEELRRKIQELFHVEPGLQRLFYRGK
QMEDGHTLFDYEVRLNDTIQLLVRQSLVLPHSTKERDSELSDTDSGCCLG
QSESDKSSTHGEAAAETDSRPADEDMWDETELGLYKVNEYVDARDTNMGA
WFEAQVVRVTRKAPSRDEPCSSTSRPALEEDVIYHVKYDDYPENGVVQMN
SRDVRARARTIIKWQDLEVGQVVMLNYNPDNPKERGFWYDAEISRKRETR
TARELYANVVLGDDSLNDCRIIFVDEVFKIERPGEGSPMVDNPMRRKSGP
SCKHCKDDVNRLCRVCACHLCGGRQDPDKQLMCDECDMAFHIYCLDPPLS
SVPSEDEWYCPECRNDASEVVLAGERLRESKKKAKMASATSSSQRDWGKG
MACVGRTKECTIVPSNHYGPIPGIPVGTMWRFRVQVSESGVHRPHVAGIH
GRSNDGAYSLVLAGGYEDDVDHGNFFTYTGSGGRDLSGNKRTAEQSCDQK
LTNTNRALALNCFAPINDQEGAEAKDWRSGKPVRVVRNVKGGKNSKYAPA
EGNRYDGIYKVVKYWPEKGKSGFLVWRYLLRRDDDEPGPWTKEGKDRIKK
LGLTMQYPEGYLEALANREREKENSKREEEEQQEGGFASPRTGKGKWKRK
SAGGGPSRAGSPRRTSKKTKVEPYSLTAQQSSLIREDKSNAKLWNEVLAS
LKDRPASGSPFQLFLSKVEETFQCICCQELVFRPITTVCQHNVCKDCLDR
SFRAQVFSCPACRYDLGRSYAMQVNQPLQTVLNQLFPGYGNGR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU23895
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent mutations at Ala114, Asp469 and Tyr555 compared to updated NCBI ref NM_001048201.1