cDNA Clone WNK1

• Synonyms: HSAN2, HSN2, KDP, PRKWNK1, PSK, p65
• Plasmid: pEF1a GST WNK1
• Parent Plasmid: pEF1aD GST1
• Expressed: GST-WNK1
• Genbank: NM_018979.3
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCGCCATGTCTGGCGGCGCCGCAGAGAAGCAGAGCAGCACTCCCGG
TTCCCTGTTCCTCTCGCCGCCGGCTCCTGCCCCCAAGAATGGCTCCAGCT
CCGATTCCTCCGTGGGGGAGAAACTGGGAGCCGCGGCCGCCGACGCTGTG
ACCGGCAGGACCGAGGAGTACAGGCGCCGCCGCCACACTATGGACAAGGA
CAGCCGTGGGGCGGCCGCGACCACTACCACCACTGAGCACCGCTTCTTCC
GCCGGAGCGTCATCTGCGACTCCAATGCCACTGCACTGGAGCTTCCCGGC
CTTCCTCTTTCCCTGCCCCAGCCCAGCATCCCCGCGGCTGTCCCGCAGAG
TGCTCCACCGGAGCCCCACCGGGAAGAGACCGTGACCGCCACCGCCACTT
CCCAGGTAGCCCAGCAGCCTCCAGCCGCTGCCGCCCCTGGGGAACAGGCC
GTCGCGGGCCCTGCCCCCTCGACTGTCCCCAGCAGTACCAGCAAAGACCG
CCCAGTGTCCCAGCCTAGCCTTGTGGGGAGCAAAGAGGAGCCGCCGCCGG
CGAGAAGTGGCAGCGGCGGCGGCAGCGCCAAGGAGCCACAGGAGGAACGG
AGCCAGCAGCAGGATGATATCGAAGAGCTGGAGACCAAGGCCGTGGGAAT
GTCTAACGATGGCCGCTTTCTCAAGTTTGACATCGAAATCGGCAGAGGCT
CCTTTAAGACGGTCTACAAAGGTCTGGACACTGAAACCACCGTGGAAGTC
GCCTGGTGTGAACTGCAGGATCGAAAATTAACAAAGTCTGAGAGGCAGAG
ATTTAAAGAAGAAGCTGAAATGTTAAAAGGTCTTCAGCATCCCAATATTG
TTAGATTTTATGATTCCTGGGAATCCACAGTAAAAGGAAAGAAGTGCATT
GTTTTGGTGACTGAACTTATGACGTCTGGAACACTTAAAACGTATCTGAA
AAGGTTTAAAGTGATGAAGATCAAAGTTCTAAGAAGCTGGTGCCGTCAGA
TCCTTAAAGGTCTTCAGTTTCTTCATACTCGAACTCCACCTATCATTCAC
CGCGATCTTAAATGTGACAACATCTTTATCACCGGCCCTACTGGCTCAGT
CAAGATTGGAGACCTCGGTCTGGCAACCCTGAAGCGGGCTTCTTTTGCCA
AGAGTGTGATAGGTACCCCAGAGTTCATGGCCCCTGAGATGTATGAGGAG
AAATATGATGAATCCGTTGACGTTTATGCTTTTGGGATGTGCATGCTTGA
GATGGCTACATCTGAATATCCTTACTCGGAGTGCCAAAATGCTGCGCAGA
TCTACCGTCGCGTGACCAGTGGGGTGAAGCCAGCCAGTTTTGACAAAGTA
GCAATTCCTGAAGTGAAGGAAATTATTGAAGGATGCATACGACAAAACAA
AGATGAAAGATATTCCATCAAAGACCTTTTGAACCATGCCTTCTTCCAAG
AGGAAACAGGAGTACGGGTAGAATTAGCAGAAGAAGATGATGGAGAAAAA
ATAGCCATAAAATTATGGCTACGTATTGAAGATATTAAGAAATTAAAGGG
AAAATACAAAGATAATGAAGCTATTGAGTTTTCTTTTGATTTAGAGAGAG
ATGTCCCAGAAGATGTTGCACAAGAAATGGTAGAGTCTGGGTATGTCTGT
GAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGACAGAGTATCATT
AATTAAGAGGAAACGAGAGCAGCGGCAGTTGGTACGGGAGGAGCAAGAAA
AAAAAAAGCAGGAAGAGAGCAGTCTCAAACAGCAGGTAGAACAATCCAGT
GCTTCCCAGACAGGAATCAAGCAGCTCCCTTCTGCTAGCACCGGCATACC
TACTGCTTCTACCACTTCAGCTTCAGTTTCTACACAAGTAGAACCTGAAG
AACCTGAGGCAGATCAACATCAGCAACTACAGTACCAGCAACCCAGTATA
TCTGTGTTATCTGATGGGACGGTTGACAGTGGTCAGGGATCCTCTGTCTT
CACAGAATCTCGAGTGAGCAGCCAACAGACAGTTTCATATGGTTCCCAAC
ATGAACAGGCACATTCTACAGGCACAGTCCCAGGGCATATACCTTCTACT
GTCCAAGCACAGTCTCAGCCCCATGGGGTATATCCACCCTCAAGTGTGGC
ACAGGGGCAGAGCCAGGGTCAGCCATCCTCAAGTAGCTTAACAGGGGTTT
CATCTTCCCAACCCATACAACATCCTCAGCAGCAGCAGGGAATACAGCAG
ACAGCCCCTCCTCAACAGACAGTGCAGTATTCACTTTCACAGACATCAAC
CTCCAGTGAGGCCACTACTGCACAGCCAGTGAGTCAACCTCAAGCTCCAC
AAGTCTTGCCTCAAGTATCAGCTGGAAAACAGCTTCCAGTTTCCCAGCCA
GTACCAACTATCCAAGGCGAACCTCAGATCCCAGTTGCGACACAACCCTC
GGTTGTTCCAGTCCACTCTGGTGCTCATTTCCTTCCAGTGGGACAGCCGC
TCCCTACTCCCTTGCTCCCTCAGTACCCTGTCTCTCAGATTCCCATATCA
ACTCCTCATGTGTCTACGGCTCAGACAGGTTTCTCATCCCTTCCCATCAC
AATGGCAGCTGGCATTACTCAGCCTCTGCTCACGTTGGCTTCATCTGCTA
CAACAGCTGCGATCCCGGGGGTATCAACTGTGGTTCCTAGTCAGCTTCCA
ACCCTTCTGCAGCCTGTGACTCAGCTGCCAAGTCAGGTTCACCCACAGCT
CCTACAACCAGCAGTTCAGTCCATGGGAATACCAGCTAACCTTGGACAAG
CTGCTGAGGTTCCACTTTCCTCTGGAGATGTTCTGTACCAGGGCTTCCCA
CCTCGACTGCCACCACAGTACCCAGGAGATTCAAATATTGCTCCCTCTTC
CAACGTGGCTTCTGTTTGCATCCATTCTACAGTCCTATCCCCTCCCATGC
CGACAGAAGTACTGGCTACACCTGGGTACTTTCCCACAGTGGTGCAGCCT
TATGTGGAATCAAATCTTTTAGTTCCTATGGGTGGTGTAGGAGGACAGGT
TCAAGTGTCCCAGCCAGGAGGGAGTTTAGCACAAGCCCCCACTACATCCT
CCCAGCAAGCAGTTTTGGAGAGTACTCAGGGAGTCTCTCAGGTTGCTCCT
GCAGAGCCAGTTGCAGTAGCACAGCCCCAAGCTACCCAGCCGACCACTTT
GGCTTCCTCTGTAGACAGTGCACATTCAGATGTTGCTTCAGGTATGAGTG
ATGGCAATGAGAACGTCCCATCTTCCAGTGGAAGGCATGAAGGAAGAACT
ACAAAACGGCATTACCGAAAATCTGTAAGGAGTCGCTCTCGACATGAAAA
AACTTCACGCCCAAAATTAAGAATTTTGAATGTTTCAAATAAAGGAGACC
GAGTAGTAGAATGTCAATTAGAGACTCATAATAGGAAAATGGTTACATTC
AAATTTGACCTAGATGGTGACAACCCCGAGGAGATAGCAACAATTATGGT
GAACAATGACTTTATTCTAGCAATAGAGAGAGAGTCGTTTGTGGATCAAG
TGCGAGAAATTATTGAAAAAGCTGATGAAATGCTCAGTGAGGATGTCAGT
GTGGAACCAGAGGGTGATCAGGGATTGGAGAGTCTACAAGGAAAGGATGA
CTATGGCTTTTCAGGTTCTCAGAAGCTTGAAGGAGAGTTCAAACAACCAA
TTCCTGCGTCTTCCATGCCACAGCAAATAGGCATTCCTACCAGTTCTTTA
ACTCAAGTTGTTCATTCTGCGGGAAGGCGGTTTATAGTGAGTCCTGTGCC
AGAAAGCCGATTACGAGAATCAAAAGTTTTCCCCAGTGAAATAACAGATA
CAGTTGCTGCCTCTACAGCTCAGAGCCCTGGAATGAACTTGTCTCACTCT
GCATCATCCCTTAGTCTACAACAGGCCTTTTCTGAACTTAGACGTGCCCA
AATGACAGAAGGACCCAACACAGCACCTCCAAACTTTAGTCATACAGGAC
CAACATTTCCAGTAGTACCTCCTTTCTTAAGTAGCATTGCTGGAGTCCCA
ACCACAGCAGCAGCCACAGCACCAGTCCCTGCAACAAGCAGCCCTCCTAA
TGACATTTCCACATCAGTAATTCAGTCTGAGGTTACAGTGCCCACTGAAG
AGGGGATTGCTGGAGTTGCCACCAGCACAGGTGTGGTAACTTCGGGTGGT
CTCCCCATACCACCTGTGTCTGAATCACCAGTACTTTCCAGCGTAGTTTC
AAGTATCACAATACCTGCAGTTGTCTCAATATCTACTACATCCCCGTCAC
TTCAAGTCCCCACATCCACATCTGAGATCGTTGTTTCTAGTACAGCACTG
TATCCTTCAGTAACAGTTTCAGCAACTTCAGCCTCTGCAGGGGGCAGTAC
TGCTACCCCAGGTCCTAAGCCTCCAGCTGTAGTATCTCAGCAGGCAGCAG
GCAGCACTACTGTGGGAGCCACATTAACATCAGTTTCTACCACCACTTCA
TTCCCAAGCACAGCTTCACAGCTGTCCATTCAGCTTAGCAGCAGTACTTC
TACTCCTACTTTAGCTGAAACCGTGGTAGTTAGCGCACACTCACTAGATA
AGACATCTCATAGCAGTACAACTGGATTGGCTTTCTCCCTCTCTGCACCA
TCTTCCTCTTCCTCTCCTGGAGCAGGAGTGTCTAGTTATATTTCTCAGCC
TGGTGGGCTGCATCCTTTGGTCATTCCATCAGTGATAGCTTCTACTCCTA
TTCTTCCCCAAGCAGCAGGACCTACTTCTACACCTTTATTACCCCAAGTA
CCTAGTATCCCACCCTTGGTACAGCCTGTTGCCAATGTGCCTGCTGTACA
GCAGACACTAATTCATAGTCAGCCTCAACCAGCTTTGCTTCCCAACCAGC
CCCATACTCATTGTCCTGAAGTAGATTCTGATACACAACCCAAAGCTCCT
GGAATTGATGACATAAAGACTCTAGAAGAAAAGCTGCGGTCTCTGTTCAG
TGAACACAGCTCATCTGGAGCTCAGCATGCCTCTGTCTCACTGGAGACCT
CACTAGTCATAGAGAGCACTGTCACACCAGGCATCCCAACTACTGCTGTT
GCACCAAGCAAACTCCTGACTTCTACCACAAGTACTTGCTTACCACCAAC
CAATTTACCACTAGGAACAGTTGCTTTGCCAGTTACACCAGTGGTCACAC
CTGGGCAAGTTTCTACCCCAGTCAGCACTACTACATCAGGAGTGAAACCT
GGAACTGCTCCCTCCAAGCCACCTCTAACTAAGGCTCCGGTGCTGCCAGT
GGGTACTGAACTTCCAGCAGGTACTCTACCCAGCGAGCAGCTGCCACCTT
TTCCAGGACCTTCTCTAACCCAGTCCCAGCAACCTCTAGAGGATCTTGAT
GCTCAATTGAGAAGAACACTTAGTCCAGAGATTATCACAGTGACTTCTGC
GGTTGGTCCTGTGTCCATGGCGGCTCCAACAGCAATCACAGAAGCAGGAA
CACAGCCTCAGAAGGGTGTTTCTCAAGTCAAAGAAGGCCCTGTCCTAGCG
ACTAGTTCAGGAGCTGGTGTTTTTAAGATGGGACGATTTCAGGTTTCTGT
TGCAGCAGACGGTGCCCAGAAAGAGGGTAAAAATAAGTCAGAAGATGCAA
AGTCTGTTCATTTTGAATCCAGCACCTCAGAGTCCTCAGTGCTATCAAGT
AGTAGTCCAGAGAGTACCTTGGTGAAACCAGAGCCGAATGGCATAACCAT
CCCTGGTATCTCTTCAGATGTGCCAGAGAGTGCCCACAAAACTACTGCCT
CAGAGGCAAAGTCAGACACTGGGCAGCCTACCAAGGTTGGACGTTTTCAG
GTGACAACTACAGCAAACAAAGTGGGTCGTTTCTCTGTATCAAAAACTGA
GGACAAGATCACTGACACAAAGAAAGAAGGACCAGTGGCATCTCCTCCTT
TTATGGATTTGGAACAAGCTGTTCTTCCTGCTGTGATACCAAAGAAAGAG
AAGCCTGAACTGTCAGAGCCTTCACATCTAAATGGGCCGTCTTCTGACCC
GGAGGCCGCTTTTTTAAGTAGGGATGTGGATGATGGTTCCGGTAGTCCAC
ACTCGCCCCATCAGCTGAGCTCAAAGAGCCTTCCTAGCCAGAATCTAAGT
CAAAGCCTTAGTAATTCATTTAACTCCTCTTACATGAGTAGCGACAATGA
GTCAGATATCGAAGATGAAGACTTAAAGTTAGAGCTGCGACGACTACGAG
ATAAACATCTCAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAA
ATTGAATCTTTGTATACCAAACTGGGCAAGGTGCCCCCTGCTGTTATTAT
TCCCCCAGCTGCTCCCCTTTCAGGGAGAAGACGACGACCCACTAAAAGCA
AAGGCAGCAAATCTAGTCGAAGCAGTTCCTTGGGGAATAAAAGCCCCCAG
CTTTCAGGTAACCTGTCTGGTCAGAGTGCAGCTTCAGTCTTGCACCCCCA
GCAGACCCTCCACCCTCCTGGCAACATCCCAGAGTCCGGGCAGAATCAGC
TGTTACAGCCCCTTAAGCCATCTCCCTCCAGTGACAACCTCTATTCAGCC
TTCACCAGTGATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCA
AGGAACCAGCAGCACAAACACTGTTGGGGCAACAGTGAACAGCCAAGCCG
CCCAAGCTCAGCCTCCTGCCATGACGTCCAGCAGGAAGGGCACATTCACA
GATGACTTGCACAAGTTGGTAGACAATTGGGCCCGAGATGCCATGAATCT
CTCAGGCAGGAGAGGAAGCAAAGGGCACATGAATTATGAGGGCCCTGGAA
TGGCAAGGAAGTTCTCTGCACCTGGGCAACTGTGCATCTCCATGACCTCG
AACCTGGGTGGCTCTGCCCCCATCTCTGCAGCATCAGCTACCTCTCTAGG
TCACTTCACCAAGTCTATGTGCCCCCCACAGCAGTATGGCTTTCCAGCTA
CCCCATTTGGCGCTCAATGGAGTGGGACGGGTGGCCCAGCACCACAGCCA
CTTGGCCAGTTCCAACCTGTGGGAACTGCCTCCTTGCAGAATTTCAACAT
CAGCAATTTGCAGAAATCCATCAGCAACCCCCCAGGCTCCAACCTGCGGA
CCACTTAGGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGEFAMSGGAAEKQSSTPG
SLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKD
SRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQS
APPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDR
PVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGM
SNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQR
FKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLK
RFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSV
KIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLE
MATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNK
DERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKG
KYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSL
IKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIP
TASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVF
TESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVA
QGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTST
SSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPS
VVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPIT
MAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQL
LQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSS
NVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQV
QVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQPQATQPTTL
ASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEK
TSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMV
NNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDD
YGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVP
ESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQ
MTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPN
DISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVS
SITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGST
ATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTS
TPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQP
GGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQ
QTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFS
EHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPT
NLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPV
GTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSA
VGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSV
AADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITI
PGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTE
DKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDP
EAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNE
SDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVII
PPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQ
QTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQ
GTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNL
SGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLG
HFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNI
SNLQKSISNPPGSNLRTT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44385
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains several silent variations as well as two benign residue changes (according to ensemble); T1056P and M1808I.; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.