cDNA Clone UBQLN3

• Plasmid: pMAL6P-1-UBQLN3 aa364-497
• Parent Plasmid: pMAL6P
• Expressed: MBP-UBQLN3 aa364-497
• Genbank: NM_198623.2
• Tag: MBP
• Species: Mouse

Sequence of Insert

GGATCCcagggtactgtgccaacttcaaatccaagccaggaatcaccactttcaggaaacagagttcctccaactttaccatcatcccctaaatctgggtcaggccaatctctgcccaaagagtcagtagcaatcaagggaaagtcatcatgcccagctttcttgagacactcaactgagaacagtactggacaaggtggaagcctacatgatgcagggaagggctctactggccccagtactagcttgcccaatcttacctctcagataggagattctgccaacagatcctcgtttgtttctacaccatcttcccttatgtcagctactcctggagtcccagaatctccctggcttcctccaacaggttactcaagatctctgagatcagcgggcacaaatcaagtctgaGCGGCCGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGIEGRISELALEVLFQGPWGSQGTVPTSNPSQESPLSGNRVPPTLPSSPKSGSGQSLPKESVAIKGKSSCPAFLRHSTENSTGQGGSLHDAGKGSTGPSTSLPNLTSQIGDSANRSSFVSTPSSLMSATPGVPESPWLPPTGYSRSLRSAGTNQV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU23484
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: clone harbours silent mutations at Phe415 (c1245t) and Thr424 (t1272c)