cDNA Clone BTRC

• Synonyms: BETA-TRCP, FBW1A, FBXW1, FBXW1A, FWD1, bTrCP, bTrCP1, betaTrCP
• Plasmid: pET15b 6xHIS SUMO TEVb BTRC
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6xHIS-SUMO-TEVb-BTRC
• Genbank: NM_033637
• Tag: SUMO
• Species: Human

Sequence of Insert

GGATCCGAAAACCTGTATTTTCAGATGGACCCGGCCGAGGCGGTGCTGCAAGAGAAGGCACTCAAGTTTATGTGCTCTATGCCCAGGTCTCTGTGGCTGGGCTGCTCCAGCCTGGCGGACAGCATGCCTTCGCTGCGATGCCTGTATAACCCAGGGACTGGCGCACTCACAGCTTTCCAGAATTCCTCAGAGAGAGAAGACTGTAATAATGGCGAACCCCCTAGGAAGATAATACCAGAGAAGAATTCACTTAGACAGACATACAACAGCTGTGCCAGACTCTGCTTAAACCAAGAAACAGTATGTTTAGCAAGCACTGCTATGAAGACTGAGAATTGTGTGGCCAAAACAAAACTTGCCAATGGCACTTCCAGTATGATTGTGCCCAAGCAACGGAAACTCTCAGCAAGCTATGAAAAGGAAAAGGAACTGTGTGTCAAATACTTTGAGCAGTGGTCAGAGTCAGATCAAGTGGAATTTGTGGAACATCTTATATCCCAAATGTGTCATTACCAACATGGGCACATAAACTCGTATCTTAAACCTATGTTGCAGAGAGATTTCATAACTGCTCTGCCAGCTCGGGGATTGGATCATATTGCTGAGAACATTCTGTCATACCTGGATGCCAAATCACTATGTGCTGCTGAACTTGTGTGCAAGGAATGGTACCGAGTGACCTCTGATGGCATGCTGTGGAAGAAGCTTATCGAGAGAATGGTCAGGACAGATTCTCTGTGGAGAGGCCTGGCAGAACGAAGAGGATGGGGACAGTATTTATTCAAAAACAAACCTCCTGACGGGAATGCTCCTCCCAACTCTTTTTATAGAGCACTTTATCCTAAAATTATACAAGACATTGAGACAATAGAATCTAATTGGAGATGTGGAAGACATAGTTTACAGAGAATTCACTGCCGAAGTGAAACAAGCAAAGGAGTTTACTGTTTACAGTATGATGATCAGAAAATAGTAAGCGGCCTTCGAGACAACACAATCAAGATCTGGGATAAAAACACATTGGAATGCAAGCGAATTCTCACAGGCCATACAGGTTCAGTCCTCTGTCTCCAGTATGATGAGAGAGTGATCATAACAGGATCATCGGATTCCACGGTCAGAGTGTGGGATGTAAATACAGGTGAAATGCTAAACACGTTGATTCACCATTGTGAAGCAGTTCTGCACTTGCGTTTCAATAATGGCATGATGGTGACCTGCTCCAAAGATCGTTCCATTGCTGTATGGGATATGGCCTCCCCAACTGACATTACCCTCCGGAGGGTGCTGGTCGGACACCGAGCTGCTGTCAATGTTGTAGACTTTGATGACAAGTACATTGTTTCTGCATCTGGGGATAGAACTATAAAGGTATGGAACACAAGTACTTGTGAATTTGTAAGGACCTTAAATGGACACAAACGAGGCATTGCCTGTTTGCAGTACAGGGACAGGCTGGTAGTGAGTGGCTCATCTGACAACACTATCAGATTATGGGACATAGAATGTGGTGCATGTTTACGAGTGTTAGAAGGCCATGAGGAATTGGTGCGTTGTATTCGATTTGATAACAAGAGGATAGTCAGTGGGGCCTATGATGGAAAAATTAAAGTGTGGGATCTTGTGGCTGCTTTGGACCCCCGTGCTCCTGCAGGGACACTCTGTCTACGGACCCTTGTGGAGCATTCCGGAAGAGTTTTTCGACTACAGTTTGATGAATTCCAGATTGTCAGTAGTTCACATGATGACACAATCCTCATCTGGGACTTCCTAAATGATCCAGCTGCCCAAGCTGAACCCCCCCGTTCCCCTTCTCGAACATACACCTACATCTCCAGATAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGSENLYFQMDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCLNQETVCLASTAMKTENCVAKTKLANGTSSMIVPKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDGNAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKNTLECKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNTGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPAAQAEPPRSPSRTYTYISR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44156
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cleavage at starting Methionine