cDNA Clone Ubiquitin

• Plasmid: pET28a 6HIS TEVb Ubiquitin 1-73 precission C-GFP
• Parent Plasmid: pET28a C-3C GFP
• Expressed: 6HIS TEVb Ubiquitin 1-73 precission C-GFP
• Genbank: P0CG47
• Tag: GFP
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCTCGCGGCCGC

Amino Acid Sequence

MHHHHHHENLYFQMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLAAALEVLFQGPLVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU22796
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental Plasmid DU32893; 6HIS TEV attached to insert