cDNA Clone UBQLN1

• Synonyms: Ubiquilin-1
• Plasmid: pET15 6HIS SUMO UBQLN1
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS-SUMO1-UBQLN1
• Genbank: NM_013438.4
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

ggatccATGGCCGAGAGTGGTGAAAGCGGCGGTCCTCCGGGCTCCCAGGATAGCGCCGCCGGAGCCGAAGGTGCTGGCGCCCCCGCGGCCGCTGCCTCCGCGGAGCCCAAAATCATGAAAGTCACCGTGAAGACCCCGAAGGAAAAGGAGGAATTCGCCGTGCCCGAGAATAGCTCCGTCCAGCAGTTTAAGGAAGAAATCTCTAAACGTTTTAAATCACATACTGACCAACTTGTGTTGATATTTGCTGGAAAAATTTTGAAAGATCAAGATACCTTGAGTCAGCATGGAATTCATGATGGACTTACTGTTCACCTTGTCATTAAAACACAAAACAGGCCTCAGGATCATTCAGCTCAGCAAACAAATACAGCTGGAAGCAATGTTACTACATCATCAACTCCTAATAGTAACTCTACATCTGGTTCTGCTACTAGCAACCCTTTTGGTTTAGGTGGCCTTGGGGGACTTGCAGGTCTGAGTAGCTTGGGTTTGAATACTACCAACTTCTCTGAACTACAGAGTCAGATGCAGCGACAACTTTTGTCTAACCCTGAAATGATGGTCCAGATCATGGAAAATCCCTTTGTTCAGAGCATGCTCTCAAATCCTGACCTGATGAGACAGTTAATTATGGCCAATCCACAAATGCAGCAGTTGATACAGAGAAATCCAGAAATTAGTCATATGTTGAATAATCCAGATATAATGAGACAAACGTTGGAACTTGCCAGGAATCCAGCAATGATGCAGGAGATGATGAGGAACCAGGACCGAGCTTTGAGCAACCTAGAAAGCATCCCAGGGGGATATAATGCTTTAAGGCGCATGTACACAGATATTCAGGAACCAATGCTGAGTGCTGCACAAGAGCAGTTTGGTGGTAATCCATTTGCTTCCTTGGTGAGCAATACATCCTCTGGTGAAGGTAGTCAACCTTCCCGTACAGAAAATAGAGATCCACTACCCAATCCATGGGCTCCACAGACTTCCCAGAGTTCATCAGCTTCCAGCGGCACTGCCAGCACTGTGGGTGGCACTACTGGTAGTACTGCCAGTGGCACTTCTGGGCAGAGTACTACTGCGCCAAATTTGGTGCCTGGAGTAGGAGCTAGTATGTTCAACACACCAGGAATGCAGAGCTTGTTGCAACAAATAACTGAAAACCCACAACTGATGCAAAACATGTTGTCTGCCCCCTACATGAGAAGCATGATGCAGTCACTAAGCCAGAATCCTGACCTTGCTGCACAGATGATGCTGAATAATCCCCTATTTGCTGGAAATCCTCAGCTTCAAGAACAAATGAGACAACAGCTCCCAACTTTCCTCCAACAAATGCAGAATCCTGATACACTATCAGCAATGTCAAACCCTAGAGCAATGCAGGCCTTGTTACAGATTCAGCAGGGTTTACAGACATTAGCAACGGAAGCCCCGGGCCTCATCCCAGGGTTTACTCCTGGCTTGGGGGCATTAGGAAGCACTGGAGGCTCTTCGGGAACTAATGGATCTAACGCCACACCTAGTGAAAACACAAGTCCCACAGCAGGAACCACTGAACCTGGACATCAGCAGTTTATTCAGCAGATGCTGCAGGCTCTTGCTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACCGTGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAAGGTTACTGGGCTCCCAGCCATCATAGgtcgac

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGSMAESGESGGPPGSQDSAAGAEGAGAPAAAASAEPKIMKVTVKTPKEKEEFAVPENSSVQQFKEEISKRFKSHTDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQDHSAQQTNTAGSNVTTSSTPNSNSTSGSATSNPFGLGGLGGLAGLSSLGLNTTNFSELQSQMQRQLLSNPEMMVQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHMLNNPDIMRQTLELARNPAMMQEMMRNQDRALSNLESIPGGYNALRRMYTDIQEPMLSAAQEQFGGNPFASLVSNTSSGEGSQPSRTENRDPLPNPWAPQTSQSSSASSGTASTVGGTTGSTASGTSGQSTTAPNLVPGVGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDLAAQMMLNNPLFAGNPQLQEQMRQQLPTFLQQMQNPDTLSAMSNPRAMQALLQIQQGLQTLATEAPGLIPGFTPGLGALGSTGGSSGTNGSNATPSENTSPTAGTTEPGHQQFIQQMLQALAGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU22818
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Needs linker sequence after senp cleavage deleted; Parental PLasmid DU32993