cDNA Clone USP51

• Plasmid: pLenti6.3/TO/V5 GFP-USP51 C372A
• Parent Plasmid: pLenti6/V5-D-TOPO
• Expressed: GFP-USP51 C372A
• Genbank: NM_201286.3 GI:284795364 with silent mutations: t1803c (His601), t1935c (Asn645)
• Tag: GFP
• Species: Human

Sequence of Insert

ggACAAGTTTGTACAAAAAAGCAGGCTgccaccATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGtccggactcggatctATGGCCCAGGTTCGAGAAACTTCTTTGCCCTCCGGCTCTGGGGTCCGCTGGATCTCCGGAGGTGGGGGAGGAGCCTCTCCTGAGGAGGCGGTTGAGAAGGCGGGGAAAATGGAGGAGGCGGCGGCGGGGGCTACGAAGGCGTCTTCGAGACGTGAAGCCGAGGAGATGAAGCTGGAGCCATTACAAGAGCGTGAGCCCGCGCCGGAGGAGAACTTGACGTGGAGCAGCAGCGGCGGCGACGAGAAGGTGCTCCCTTCAATCCCCCTTCGCTGTCACAGCAGCTCCTCGCCCGTTTGCCCGCGCCGCAAGCCCCGCCCTCGGCCCCAGCCCCGGGCCCGCTCCCGCAGCCAGCCTGGGCTCTCGGCCCCACCCCCGCCTCCAGCCCGGCCCCCGCCCCCGCCGCCACCCCCGCCCCCACCCGCACCGCGGCCCAGGGCCTGGCGTGGATCCCGGCGCAGATCCCGGCCTGGGTCCAGGCCTCAGACACGGAGAAGCTGCTCTGGTGACCTAGACGGGTCGGGGGATCCTGGCGGCTTAGGGGACTGGTTGCTGGAGGTCGAGTTTGGTCAGGGTCCCACAGGCTGCTCTCATGTGGAGAGCTTTAAAGTAGGTAAGAACTGGCAGAAGAACCTGAGGTTGATCTACCAGCGTTTCGTTTGGAGTGGGACCCCAGAGACTAGGAAACGTAAAGCAAAGTCATGCATCTGTCACGTATGTAGTACCCATATGAACAGACTCCACTCTTGTCTCTCCTGTGTCTTTTTTGGCTGCTTCACTGAGAAACATATTCACAAACATGCAGAAACAAAGCAGCACCATTTAGCTGTAGACCTTTATCATGGGGTCATATATTGCTTCATGTGTAAGGATTATGTATATGACAAAGACATAGAACAGATTGCCAAAGAAACAAAAGAAAAAATTTTGAGATTATTAACTTCCACCTCAACAGATGTTTCTCATCAACAGTTTATGACATCAGGGTTTGAAGACAAGCAATCAACCTGTGAGACAAAGGAACAGGAGCCAAAATTGGTGAAACCCAAGAAAAAGAGAAGAAAAAAGTCAGTCTATACTGTAGGCCTGAGAGGGCTAATCAATCTTGGGAACACTgcTTTTATGAATTGTATTGTCCAGGCACTTACCCATATTCCTCTACTGAAAGATTTCTTCCTCTCTGACAAGCACAAATGTATAATGACAAGCCCCAGCTTGTGTCTGGTCTGTGAAATGTCTTCGCTTTTTCATGCTATGTACTCTGGGAGCCGAACTCCTCACATTCCCTATAAGTTACTGCATCTGATATGGATCCATGCAGAACATTTAGCAGGGTACAGGCAGCAGGATGCCCATGAGTTCCTTATTGCAATATTAGACGTGCTACATAGACACAGCAAAGATGATAGTGGTGGGCAGGAGGCCAATAACCCCAACTGCTGTAACTGCATCATAGACCAAATCTTTACAGGTGGCCTGCAATCAGATGTCACATGTCAAGCCTGCCATAGTGTTTCTACCACCATAGACCCATGCTGGGACATCAGTTTGGACTTGCCTGGCTCTTGTGCCACATTCGATTCCCAGAACCCAGAGAGGGCTGACAGCACAGTGAGCAGGGATGACCACATACCAGGAATCCCCTCACTTACAGACTGTCTACAGTGGTTTACAAGGCCAGAGCACCTAGGAAGCAGTGCCAAAATCAAATGCAATAGTTGCCAAAGCTACCAGGAGTCTACTAAACAGCTCACAATGAAAAAATTACCCATTGTGGCTTGTTTTCATCTCAAGCGGTTTGAGCACGTAGGCAAACAGAGGCGAAAGATTAATACCTTTATCTCCTTTCCCTTGGAGCTGGACATGACTCCGTTTTTGGCCTCTACTAAAGAGAGCAGAATGAAAGAAGGCCAGCCACCAACAGATTGTGTGCCCAACGAGAATAAGTATTCCTTGTTTGCAGTGATTAATCACCATGGAACTTTGGAAAGTGGCCACTATACCAGCTTCATCCGGCAACAAAAGGACCAGTGGTTCAGCTGTGATGATGCCATCATCACCAAGGCTACCATTGAGGACTTACTCTACAGTGAAGGGTATTTACTGTTCTATCACAAACAGGGTCTAGAGAAAGACTAGACCCAGCTTTCTTGTACAAAGTGGTcc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAQVRETSLPSGSGVRWISGGGGGASPEEAVEKAGKMEEAAAGATKASSRREAEEMKLEPLQEREPAPEENLTWSSSGGDEKVLPSIPLRCHSSSSPVCPRRKPRPRPQPRARSRSQPGLSAPPPPPARPPPPPPPPPPPAPRPRAWRGSRRRSRPGSRPQTRRSCSGDLDGSGDPGGLGDWLLEVEFGQGPTGCSHVESFKVGKNWQKNLRLIYQRFVWSGTPETRKRKAKSCICHVCSTHMNRLHSCLSCVFFGCFTEKHIHKHAETKQHHLAVDLYHGVIYCFMCKDYVYDKDIEQIAKETKEKILRLLTSTSTDVSHQQFMTSGFEDKQSTCETKEQEPKLVKPKKKRRKKSVYTVGLRGLINLGNTAFMNCIVQALTHIPLLKDFFLSDKHKCIMTSPSLCLVCEMSSLFHAMYSGSRTPHIPYKLLHLIWIHAEHLAGYRQQDAHEFLIAILDVLHRHSKDDSGGQEANNPNCCNCIIDQIFTGGLQSDVTCQACHSVSTTIDPCWDISLDLPGSCATFDSQNPERADSTVSRDDHIPGIPSLTDCLQWFTRPEHLGSSAKIKCNSCQSYQESTKQLTMKKLPIVACFHLKRFEHVGKQRRKINTFISFPLELDMTPFLASTKESRMKEGQPPTDCVPNENKYSLFAVINHHGTLESGHYTSFIRQQKDQWFSCDDAIITKATIEDLLYSEGYLLFYHKQGLEKD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU40661
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: If sending out, check Gateway licencing.