cDNA Clone UBE4a

• Plasmid: pFBGSTDual6P-UBE4a
• Parent Plasmid: pFastbacDual 6P2
• Expressed: GST-UBE4a
• Genbank: NM_004788
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGCTCCCTGGCTGATGCCAAACAGTTTGCGGCAATCCAAAAAGAGCAGCTGAAGCAACAATCTGATGAACTCCCAGCTAGCCCAGATGACTCGGATAATAGCGTGTCAGAGAGCCTGGATGAATTCGATTACTCTGTGGCTGAGATTAGCCGCTCATTCCGATCACAGCAGGAAATATGTGAGCAACTCAACATCAATCACATGATCCAAAGGATCTTCCTTATTACTCTGGACAACAGTGATCCCAGCTTGAAAAGCGGGAATGGCATCCCTAGCCGTTGTGTGTATTTGGAAGAAATGGCAGTAGAGCTAGAAGATCAAGACTGGCTTGATATGAGCAATGTTGAGCAGGCCCTCTTCGCTCGCTTATTACTTCAAGATCCAGGCAACCAC
TTAATTAACATGACTTCTTCTACAACGCTAAATCTCTCTGCTGATCGAGA
TGCAGGAGAGAGGCACATTTTTTGTTACCTTTACTCCTGCTTCCAGAGAG
CCAAGGAAGAGATTACCAAAGTTCCAGAGAACCTGCTACCCTTTGCAGTG
CAGTGCAGAAACCTCACTGTGTCCAATACCCGAACAGTTCTTCTCACCCC
AGAGATCTATGTTGACCAAAACATCCATGAGCAACTGGTAGATTTGATGT
TAGAAGCCATCCAGGGAGCCCGTGAGTACATGAACAAGATCTATTTTGAA
GATGTAACTGAGTTTCTGGAAGAGGTCATTGAAGCCTTGATATTGGATGA
GGAAGTTAGAACATTTCCAGAAGTCATGATTCCAGTGTTTGATATTTTAT
TGGGCCGAATAAAAGATCTAGAGCTCTGTCAGATCCTTTTGTATGCATAT
CTGGATATTCTTCTCTATTTCACTAGGCAAAAAGATATGGCAAAGGTTTT
TGTAGAATACATTCAGCCCAAGGACCCTACCAATGGGCAAATGTACCAGA
AGACCTTGCTGGGAGTAATTCTGAGTATCTCCTGCTTATTAAAGACTCCG
GGTGTTGTAGAAAATCATGGCTACTTTTTGAATCCATCTCGTTCCAGCCC
CCAGGAGATCAAAGTACAGGAGGCCAACATCCATCAGTTCATGGCTCAGT
TCCACGAAAAGATCTACCAGATGCTGAAGAACTTACTCCAGCTCTCTCCA
GAAACCAAACACTGTATCTTGTCCTGGCTTGGAAACTGTTTGCATGCAAA
TGCAGGCCGCACCAAGATTTGGGCCAATCAGATGCCAGAAATCTTTTTCC
AAATGTATGCCTCAGATGCTTTCTTTCTGAATCTGGGTGCTGCTCTCCTG
AAGCTATGCCAGCCATTTTGCAAACCCAGATCCTCTCGGCTCCTCACCTT
TAATCCCACATACTGTGCCCTCAAGGAGTTGAATGATGAAGAACGAAAAA
TTAAAAATGTACACATGAGAGGTTTGGACAAAGAAACCTGTTTGATCCCA
GCTGTGCAGGAGCCGAAGTTTCCACAGAACTACAACCTTGTAACAGAGAA
CCTTGCTCTGACAGAGTACACCTTGTACTTGGGATTTCACAGGTTGCATG
ATCAGATGGTAAAAATCAACCAAAATCTGCATCGGCTGCAGGTTGCCTGG
CGGGATGCTCAGCAAAGTTCTAGCCCTGCTGCTGACAATCTTCGTGAGCA
GTTTGAACGACTGATGACCATCTATCTTTCTACCAAGACTGCCATGACAG
AGCCACAAATGCTACAAAACTGCCTAAACTTGCAGGTGTCCATGGCTGTT
CTACTGGTTCAACTGGCCATAGGCAATGAGGGCTCACAGCCAATAGAGCT
AACCTTTCCTTTGCCAGATGGCTACAGCTCTTTGGCTTATGTGCCAGAAT
TTTTTGCAGATAACCTGGGTGATTTTCTCATTTTTCTCCGCCGCTTTGCC
GATGACATTTTGGAGACATCAGCAGATTCCCTGGAGCATGTCCTTCACTT
TATCACCATTTTCACTGGAAGCATAGAAAGAATGAAGAATCCCCACCTGA
GGGCCAAACTAGCAGAGGTGTTGGAAGCAGTGATGCCCCACCTGGATCAG
ACCCCAAATCCCTTGGTATCCAGTGTGTTCCACCGGAAACGTGTGTTCTG
CAACTTTCAGTATGCACCCCAACTTGCAGAGGCTCTAATCAAGGTTTTTG
TGGACATCGAATTTACAGGAGACCCCCATCAATTTGAACAGAAGTTTAAT
TACCGCCGTCCCATGTATCCTATCCTAAGATACATGTGGGGGACAGATAC
CTATCGGGAGAGCATTAAGGATTTGGCTGACTATGCCTCTAAGAATTTAG
AAGCCATGAATCCCCCACTTTTCCTCCGCTTTCTTAACCTGCTAATGAAT
GATGCCATCTTCCTTTTGGATGAAGCCATACAGTATTTGAGCAAGATAAA
GATTCAGCAAATTGAGAAGGATCGAGGTGAATGGGATAGTCTGACTCCAG
AAGCCCGCCGAGAAAAGGAGGCTGGCCTACAGATGTTTGGACAGCTGGCA
CGTTTCCATAACATCATGTCCAATGAAACAATCGGTACCCTTGCCTTTCT
CACATCAGAGATCAAGTCACTCTTTGTGCATCCCTTCCTGGCTGAGCGCA
TCATCTCCATGTTGAACTACTTCCTGCAACACCTGGTTGGCCCCAAGATG
GGTGCCTTAAAAGTCAAGGACTTCAGCGAATTTGACTTCAAACCCCAGCA
GCTTGTATCAGATATCTGCACTATCTACTTAAATCTTGGGGATGAGGAGA
ATTTCTGTGCCACTGTGCCCAAGGATGGACGTTCCTATTCCCCAACTCTC
TTTGCACAGACAGTTCGAGTCTTGAAGAAAATAAATAAGCCTGGGAATAT
GATTATGGCTTTCAGCAACTTGGCAGAGAGAATCAAGTCTCTTGCAGACC
TCCAACAACAGGAAGAGGAAACCTATGCAGATGCCTGTGATGAGTTCCTG
GATCCCATTATGAGCACACTGATGTGTGACCCTGTGGTGCTGCCATCTTC
CAGAGTCACTGTGGATAGATCCACCATTGCAAGACATTTGCTCAGTGACC
AAACAGATCCCTTTAACCGTAGTCCCCTCACCATGGACCAGATCCGGCCA
AACACAGAACTAAAAGAAAAAATCCAACGGTGGCTTGCAGAGAGGAAACA
ACAAAAGGAGCAACTTGAATAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMTDQENNN
NISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSESL
DEFDYSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNG
IPSRCVYLEEMAVELEDQDWLDMSNVEQALFARLLLQDPGNHLINMTSST
TLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVS
NTRTVLLTPEIYVDQNIHEQLVDLMLEAIQGAREYMNKIYFEDVTEFLEE
VIEALILDEEVRTFPEVMIPVFDILLGRIKDLELCQILLYAYLDILLYFT
RQKDMAKVFVEYIQPKDPTNGQMYQKTLLGVILSISCLLKTPGVVENHGY
FLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCILS
WLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCK
PRSSRLLTFNPTYCALKELNDEERKIKNVHMRGLDKETCLIPAVQEPKFP
QNYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSS
PAADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIG
NEGSQPIELTFPLPDGYSSLAYVPEFFADNLGDFLIFLRRFADDILETSA
DSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVSS
VFHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPI
LRYMWGTDTYRESIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFLLDE
AIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFGQLARFHNIMSN
ETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDF
SEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVL
KKINKPGNMIMAFSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLM
CDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKI
QRWLAERKQQKEQLE

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU20026
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express Ube4a with N-terminal GST tag (contains PreScission protease site); ; Estimated MW of expressed protein : 151326 Daltons