cDNA Clone USP11

• Plasmid: pGEX6P-2-USP11
• Parent Plasmid: pGEX6P2
• Expressed: GST-USP11
• Genbank: NM_004651
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCTATGGCAGTAGCCCCGCGACTGTTTGGGGGGCTCTGCTTCCGTTTCCGGGACCAGAATCCGGAAGTGGCTGTTGAGGGGCGTCTTCCAATCTCGCACAGCTGCGTTGGCTGTAGAAGAGAACGGACGGCGATGGCGACGGTCGCAGCAAATCCAGCTGCTGCTGCGGCGGCTGTGGCGGCGGCAGCGGCGGTGACTGAGGATAGAGAGCCACAGCACGAGGAGCTGCCAGGCCTGGACAGCCAGTGGCGCCAGATAGAAAACGGCGAGAGTGGGCGAGAACGTCCACTGCGGGCCGGCGAAAGCTGGTTCCTTGTGGAGAAGCACTGGTATAAGCAGTGGGAGGCATACGTGCAGGGAGGGGACCAGGACTCCAGCACCTTCCCTGGCTGCATCAACAATGCCACACTCTTTCAAGATGAGATAAACTGGCGCCTCAAGGAGGGACTGGTG
GAAGGCGAGGATTATGTGCTGCTCCCAGCAGCTGCTTGGCATTACCTGGT
CAGCTGGTATGGTCTAGAGCATGGCCAGCCACCCATTGAACGCAAGGTCA
TAGAGCTGCCCAACATCCAGAAGGTCGAAGTGTACCCAGTAGAACTGCTG
CTTGTCCGGCACAATGATTTGGGCAAATCTCACACTGTTCAGTTCAGCCA
TACCGATTCTATTGGCCTAGTATTGCGCACAGCTCGGGAGCGGTTTCTGG
TGGAGCCCCAGGAAGACACTCGGCTTTGGGCCAAGAACTCAGAAGGCTCT
TTGGATAGGTTGTATGACACACACATCACGGTTCTCGATGCGGCCCTTGA
GACTGGGCAGTTGATCATCATGGAGACCCGCAAGAAAGATGGCACTTGGC
CCAGCGCACAGCTGCATGTCATGAACAACAACATGTCGGAAGAGGATGAG
GACTTCAAGGGTCAGCCAGGCATCTGTGGCCTCACCAATCTGGGCAACAC
GTGCTTCATGAACTCGGCCCTGCAGTGCCTCAGCAATGTGCCACAGCTCA
CCGAGTACTTCCTCAACAACTGCTACCTGGAGGAGCTCAACTTCCGCAAC
CCACTGGGCATGAAGGGTGAGATCGCAGAGGCCTATGCAGACCTGGTGAA
GCAGGCGTGGTCTGGCCACCACCGCTCCATTGTGCCACATGTGTTCAAGA
ACAAGGTTGGCCATTTTGCATCCCAATTTCTGGGCTACCAGCAGCATGAC
TCTCAGGAGCTGCTGTCATTCCTCCTGGACGGGCTGCATGAGGACCTTAA
TCGGGTGAAGAAGAAGGAGTATGTGGAGCTGTGCGATGCTGCTGGGCGAC
CGGATCAGGAGGTGGCACAGGAGGCATGGCAAAACCACAAACGGCGGAAC
GATTCTGTGATCGTGGACACTTTCCACGGCCTCTTCAAGTCCACGCTGGT
GTGCCCCGATTGTGGCAATGTATCTGTGACCTTCGACCCCTTCTGCTACC
TCAGTGTTCCACTGCCTATCAGCCACAAGAGGGTCTTGGAGGTCTTCTTT
ATCCCCATGGATCCGCGCCGCAAGCCAGAGCAGCACCGGCTCGTGGTCCC
CAAGAAAGGCAAGATCTCGGATCTATGTGTGGCTCTGTCCAAACACACGG
GCATCTCGCCAGAGAGGATGATGGTGGCTGATGTCTTCAGTCACCGCTTC
TATAAGCTCTATCAGCTAGAGGAGCCTCTGAGCAGCATCTTGGACCGTGA
TGATATCTTCGTCTATGAGGTGTCAGGTCGCATTGAGGCCATTGAGGGCT
CAAGAGAGGACATCGTGGTTCCTGTCTACCTGCGGGAGCGCACCCCTGCC
CGTGACTACAACAACTCCTACTACGGCCTGATGCTTTTTGGACACCCCCT
CCTGGTATCAGTGCCCCGGGACCGCTTCACCTGGGAGGGCCTGTATAACG
TCCTGATGTACCGGCTCTCACGCTACGTGACCAAACCCAACTCAGATGAT
GAGGACGATGGGGATGAGAAAGAAGATGACGAGGAGGATAAAGATGACGT
CCCTGGGCCCTCAACTGGGGGCAGCCTCCGAGACCCTGAGCCAGAGCAGG
CTGGGCCCAGCTCTGGAGTCACGAACAGGTGCCCGTTCCTCCTGGACAAT
TGCCTTGGCACATCTCAGTGGCCCCCAAGGCGACGACGCAAGCAGCTGTT
CACCCTGCAGACGGTGAACTCCAATGGGACCAGCGACCGCACAACCTCCC
CTGAAGAAGTCCATGCCCAGCCGTACATTGCTATCGACTGGGAGCCAGAG
ATGAAGAAGCGTTACTATGACGAGGTAGAGGCTGAGGGCTACGTGAAGCA
TGACTGCGTCGGGTACGTGATGAAGAAGGCTCCCGTGCGGCTGCAGGAGT
GCATTGAGCTCTTCACCACTGTGGAGACCCTGGAGAAGGAAAACCCCTGG
TACTGCCCTTCCTGCAAGCAGCACCAGCTGGCAACCAAGAAGCTGGACCT
GTGGATGCTGCCGGAGATTCTCATCATCCACCTGAAACGCTTTTCCTACA
CCAAGTTCTCCCGAGAGAAGCTGGACACCCTCGTGGAGTTTCCTATCCGG
GACCTGGACTTCTCTGAGTTTGTCATCCAGCCACAGAATGAGTCGAATCC
GGAGCTGTACAAATATGACCTCATCGCGGTTTCCAACCATTATGGGGGCA
TGCGTGATGGACACTACACAACATTTGCCTGCAACAAGGACAGCGGCCAG
TGGCACTACTTTGATGACAACAGCGTCTCCCCTGTCAATGAGAATCAGAT
CGAGTCCAAGGCAGCCTATGTCCTCTTCTACCAACGCCAGGACGTGGCGC
GACGCCTGCTGTCCCCGGCCGGCTCATCTGGCGCCCCAGCCTCCCCTGCC
TGCAGCTCCCCACCCAGCTCTGAGTTCATGGATGTTAATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMAVAPRLF
GGLCFRFRDQNPEVAVEGRLPISHSCVGCRRERTAMATVAANPAAAAAAV
AAAAAVTEDREPQHEELPGLDSQWRQIENGESGRERPLRAGESWFLVEKH
WYKQWEAYVQGGDQDSSTFPGCINNATLFQDEINWRLKEGLVEGEDYVLL
PAAAWHYLVSWYGLEHGQPPIERKVIELPNIQKVEVYPVELLLVRHNDLG
KSHTVQFSHTDSIGLVLRTARERFLVEPQEDTRLWAKNSEGSLDRLYDTH
ITVLDAALETGQLIIMETRKKDGTWPSAQLHVMNNNMSEEDEDFKGQPGI
CGLTNLGNTCFMNSALQCLSNVPQLTEYFLNNCYLEELNFRNPLGMKGEI
AEAYADLVKQAWSGHHRSIVPHVFKNKVGHFASQFLGYQQHDSQELLSFL
LDGLHEDLNRVKKKEYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDTF
HGLFKSTLVCPDCGNVSVTFDPFCYLSVPLPISHKRVLEVFFIPMDPRRK
PEQHRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEE
PLSSILDRDDIFVYEVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYY
GLMLFGHPLLVSVPRDRFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKE
DDEEDKDDVPGPSTGGSLRDPEPEQAGPSSGVTNRCPFLLDNCLGTSQWP
PRRRRKQLFTLQTVNSNGTSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDE
VEAEGYVKHDCVGYVMKKAPVRLQECIELFTTVETLEKENPWYCPSCKQH
QLATKKLDLWMLPEILIIHLKRFSYTKFSREKLDTLVEFPIRDLDFSEFV
IQPQNESNPELYKYDLIAVSNHYGGMRDGHYTTFACNKDSGQWHYFDDNS
VSPVNENQIESKAAYVLFYQRQDVARRLLSPAGSSGAPASPACSSPPSSE
FMDVN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20016
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for USP11 with N-terminal GST tag (contains preScission protease site); ; Estimated MW of expressed protein : 137621 Daltons