cDNA Clone UBE2E2

• Plasmid: pGEX6P-1-UBE2E2
• Parent Plasmid: pGEX6P1
• Expressed: GST-UBE2E2
• Genbank: NP_689866.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCCACTGAGGCACAAAGAGTTGATGACAGTCCAAGCACTAG
TGGAGGAAGTTCCGATGGAGATCAACGTGAAAGTGTTCAGCAAGAACCAG
AAAGAGAACAAGTTCAGCCCAAGAAAAAGGAGGGAAAAATATCCAGCAAA
ACCGCTGCTAAATTGTCAACTAGTGCTAAAAGAATTCAGAAGGAACTTGC
AGAAATCACATTGGACCCTCCTCCCAACTGTAGTGCTGGACCCAAAGGAG
ACAACATTTATGAATGGAGGTCAACTATATTGGGACCCCCAGGATCTGTC
TATGAAGGAGGGGTGTTCTTTCTTGACATTACCTTTTCACCAGACTATCC
GTTTAAACCCCCTAAGGTTACCTTCCGAACAAGAATCTATCACTGTAATA
TTAACAGCCAAGGTGTGATCTGTCTGGACATCTTAAAGGACAACTGGAGT
CCGGCTTTAACTATTTCTAAAGTTCTCCTCTCCATCTGCTCACTTCTTAC
AGATTGCAACCCTGCTGACCCTCTGGTGGGCAGCATCGCCACACAGTACA
TGACCAACAGAGCAGAGCATGACCGGATGGCCAGACAGTGGACCAAGCGG
TACGCCACATAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSTEAQRVDDSPSTSGGSS
DGDQRESVQQEPEREQVQPKKKEGKISSKTAAKLSTSAKRIQKELAEITL
DPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPP
KVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNP
ADPLVGSIATQYMTNRAEHDRMARQWTKRYAT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20060
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for UBE2E2 with N-terminal GST tag (contains preScission protease site); ; Estimated MW of expressed protein : 49080 Daltons