cDNA Clone UBE2U

• Plasmid: pET156P-1-UBE2U
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-UBE2U
• Genbank: Q5VVX9
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGCACGGCAGAGCTTACCTCTTGCTGCACAGAGACTTCTGTGA
TCTCAAGGAGAACAATTATAAGGGTATCACTGCTAAGCCTGTAAGTGAAG
ATATGATGGAATGGGAAGTTGAAATTGAAGGTCTACAGAATTCAGTTTGG
CAGGGTTTAGTCTTCCAACTGACAATACATTTTACATCGGAGTACAACTA
TGCTCCTCCAGTTGTGAAATTTATAACAATTCCGTTTCATCCAAATGTAG
ACCCACACACTGGTCAGCCCTGTATAGACTTTTTGGACAACCCTGAGAAG
TGGAATACAAACTATACATTGAGCAGCATCTTACTTGCCCTACAGGTTAT
GCTTTCTAATCCAGTGCTAGAGAATCCAGTGAATTTGGAAGCAGCCAGAA
TACTGGTTAAAGATGAATCTCTGTACAGAACAATTCTAAGACTTTTCAAC
AGGCCATTACAAATGAAAGATGACAGCCAGGAGTTACCTAAAGACCCACG
TAAATGTATCAGACCAATTAAAACAACCTCATTTAGTGATTACTACCAGA
CATGGTCCAGAATAGCTACATCAAAAGCCACAGAATACTACAGAACTCCA
TTGCTCAAAGTTCCAAATTTCATTGGACAGTATTACAAATGGAAGAAAAT
GGATCTACAGCATCAGAAAGAATGGAATTTAAAGTATTCCGTTATCAAGT
GTTGGCTTGCTAGAAAAAGAATGCCTCATGAAGTCACTCACTCAATGGAA
GAAATTAAGCTCTGCCCAACTCTAATTCCGACCACAGATGAAATTTTTCT
TGAGTCACCAACTGCAATAAATAGCATCACAGACATTTATGAAACAGAAG
AGGAGGGGTGGAAGAGTGACACTTCATTATATGAAAATGACACAGATGAG
CCCAGGGAAGAGGAAGTGGAAGATCTGATCTCCTGGACCAATACTCTCAA
TACAAATACTTCAGAAGATTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMHGRAYLLLHRDFCDLKENNYKGITAK
PVSEDMMEWEVEIEGLQNSVWQGLVFQLTIHFTSEYNYAPPVVKFITIPF
HPNVDPHTGQPCIDFLDNPEKWNTNYTLSSILLALQVMLSNPVLENPVNL
EAARILVKDESLYRTILRLFNRPLQMKDDSQELPKDPRKCIRPIKTTSFS
DYYQTWSRIATSKATEYYRTPLLKVPNFIGQYYKWKKMDLQHQKEWNLKY
SVIKCWLARKRMPHEVTHSMEEIKLCPTLIPTTDEIFLESPTAINSITDI
YETEEEGWKSDTSLYENDTDEPREEEVEDLISWTNTLNTNTSED*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20061
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid to express UBE2U with N-terminal 6HIS tag (contains preScission protease site); ; Estimated MW of expressed protein : 40186 Daltons