cDNA Clone Ubiquilin

• Plasmid: pGEX6P-1-UBIQUILIN TUBE
• Parent Plasmid: pGEX6P1
• Expressed: GST-ubiquilin TUBE
• Genbank: NM_053067.2
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATT
TCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACCGTGAAG
CAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATT
GAAAGGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGG
AGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATTTCAGCAACAACTGG
AACAACTCAGTGCAATGGGATTTTTGAACCGTGAAGCAAACTTGCAAGCT
CTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAAGGTTACTGGG
CTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGC
TACAGAATCCAGAAGTCAGATTTCAGCAACAACTGGAACAACTCAGTGCA
ATGGGATTTTTGAACCGTGAAGCAAACTTGCAAGCTCTAATAGCAACAGG
AGGTGATATCAATGCAGCTATTGAAAGGTTACTGGGCTCCCAGCCATCAG
GAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAA
GTCAGATTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAA
CCGTGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATG
CAGCTATTGAAAGGTTACTGGGCTCCCAGCCATCATAGGGAGGTGGAGGA
TCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSGGGVNPQLQNPEVRFQQQL
EQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPSGGGGSGGGVNPQ
LQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPS
GGGGSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDIN
AAIERLLGSQPSGGGGSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNREAN
LQALIATGGDINAAIERLLGSQPS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20100
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for Ubiquilin-based TUBE with N-terminal GST tag (contains preScission protease site); ; Estimated MW of expressed protein : 51964 Daltons