cDNA Clone UBE2S

• Plasmid: pET28-UBE2S
• Parent Plasmid: pET28a original
• Expressed: 6His-UBE2S
• Genbank: AAH65364
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCAACTCCAACGTGGAGAACCTACCCCCGCACATCATCCGCCTGG
TGTACAAGGAGGTGACGACACTGACCGCAGACCCACCCGATGGCATCAAG
GTCTTTCCCAACGAGGAGGACCTCACCGACCTCCAGGTCACCATCGAGGG
CCCTGAGGGGACCCCATATGCTGGAGGTCTGTTCCGCATGAAACTCCTGC
TGGGGAAGGACTTCCCTGCCTCCCCACCCAAGGGCTACTTCCTGACCAAG
ATCTTCCACCCGAACGTGGGCGCCAATGGCGAGATCTGCGTCAACGTGCT
CAAGAGGGACTGGACGGCTGAGCTGGGCATCCGACACGTACTGCTGACCA
TCAAGTGCCTGCTGATCCACCCTAACCCCGAGTCTGCACTCAACGAGGAG
GCGGGCCGCCTGCTCTTGGAGAACTACGAGGAGTATGCAGCTCGGGCCCG
TCTGCTCACAGAGATCCACGGGGGCGCCGGCGGGCCCAGCGGCAGGGCCG
AAGCCGGTCGGGCCCTGGCCAGTGGCACTGAAGCTTCCTCCACCGACCCT
GGGGCCCCAGGGGGCCCGGGAGGGGCTGAGGGTCCCATGGCCAAGAAGCA
TGCTGGCGAGCGCGATAAGAAGCTGGCGGCCAAGAAAAAGACGGACAAGA
AGCGGGCGCTGCGGCGGCTGTAGGCGGCCGC

Amino Acid Sequence

HHHHHHSSGLVPRGSHMASMTGGQQMGRGSNSNVENLPPHIIRLVYKEVT
TLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFP
ASPPKGYFLTKIFHPNVGANGEICVNVLKRDWTAELGIRHVLLTIKCLLI
HPNPESALNEEAGRLLLENYEEYAARARLLTEIHGGAGGPSGRAEAGRAL
ASGTEASSTDPGAPGGPGGAEGPMAKKHAGERDKKLAAKKKTDKKRALRR
L*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU20175
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express UBE2S with N-terminal 6HIS tag (contains Thrombin cleavage site); ; Estimated MW of expressed protein : 27038 Daltons