cDNA Clone UBE2D2

• Plasmid: pET28-UBE2D2
• Parent Plasmid: pET28a original
• Expressed: 6His-UBE2D2
• Genbank: NM_003339
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGCTCTGAAGAGAATCCACAAGGAATTGAATGATCTGGCACGG
GACCCTCCAGCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCA
TTGGCAAGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAG
TATTTTTCTTGACAATTCATTTCCCAACAGATTACCCCTTCAAACCACCT
AAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGTAATGG
CAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTA
TTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCA
GATGATCCTTTAGTGCCTGAGATTGCTCGGATCTACAAAACAGATAGAGA
AAAGTACAACAGAATAGCTCGGGAATGGACTCAGAAGTATGCGATGTAAG
CGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSALKRIHKELNDLARDPPAQC
SAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTT
RIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVP
EIARIYKTDREKYNRIAREWTQKYAM*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU20184
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express UBE2D2 with N-terminal 6HIS tag (contains Thrombin cleavage site); ; Estimated MW of expressed protein : 19786 Daltons