cDNA Clone UBE2W

• Plasmid: pGEX6P-1-UBE2W isoform 1
• Parent Plasmid: pGEX6P1
• Expressed: GST-UBE2W
• Genbank: NM_001001481.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGTCAATGCAGACCACAGGAAGGAGGGTGGAAGTATGGTT
TCCAAAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGACCCACCTC
CTGGAATGACCTTAAATGAGAAGAGTGTTCAAAATTCAATTACACAGTGG
ATTGTAGACATGGAAGGTGCACCAGGTACCTTATATGAAGGGGAAAAATT
TCAACTTCTATTTAAATTTAGTAGTCGATATCCTTTTGACTCTCCTCAGG
TCATGTTTACTGGTGAAAATATTCCTGTTCATCCTCATGTTTATAGCAAT
GGTCATATCTGTTTATCCATTCTAACAGAAGACTGGTCCCCAGCGCTCTC
AGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAGCTGCAAGG
AAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAG
AATCCAAAGAAAACAAAATGGTGGTATCATGATGATACTTGTTGAGCGGC
CGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMASMQTTGRRVEVWFPKRL
QKELLALQNDPPPGMTLNEKSVQNSITQWIVDMEGAPGTLYEGEKFQLLF
KFSSRYPFDSPQVMFTGENIPVHPHVYSNGHICLSILTEDWSPALSVQSV
CLSIISMLSSCKEKRRPPDNSFYVRTCNKNPKKTKWWYHDDTC*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20191
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for GST-UBE2W (with Precission protease cleavage site); ; Estimated MW of expressed protein : 45485 Daltons