cDNA Clone ADRM1

• Plasmid: pET28a-6His-DAC C3 ADRM1
• Parent Plasmid: pET28a 6His thr DAC 3C
• Expressed: 6His.DAC C3 ADRM1
• Genbank: NM_007002.2*
• Tag: DAC
• Species: Human

Sequence of Insert

GGATCCATGACGACCTCAGGCGCGCTCTTTCCAAGCCTGGTGCCAGGCTC
TCGGGGCGCCTCCAACAAGTACTTGGTGGAGTTTCGGGCGGGAAAGATGT
CCCTGAAGGGGACCACCGTGACTCCGGATAAGCGGAAAGGGCTGGTGTAC
ATTCAGCAGACGGACGACTCGCTTATTCACTTCTGCTGGAAGGACAGGAC
GTCCGGGAACGTGGAAGACGACTTGATCATCTTCCCTGACGACTGTGAGT
TCAAGCGGGTGCCGCAGTGCCCCAGCGGGAGGGTCTACGTGCTGAAGTTC
AAGGCAGGGTCCAAGCGGCTTTTCTTCTGGATGCAGGAACCCAAGACAGA
CCAGGATGAGGAGCATTGCCGGAAAGTCAACGAGTATCTGAACAACCCCC
CGATGCCTGGGGCGCTGGGGGCCAGCGGAAGCAGCGGCCACGAACTCTCT
GCGCTAGGCGGTGAGGGTGGCCTGCAGAGCCTGCTGGGAAACATGAGCCA
CAGCCAGCTCATGCAGCTCATCGGACCAGCCGGCCTCGGAGGACTGGGTG
GGCTGGGGGCCCTGACTGGACCTGGCCTGGCCAGCTTACTGGGGAGCAGT
GGGCCTCCAGGGAGCAGCTCCTCCTCCAGCTCCCGGAGCCAGTCGGCAGC
GGTCACCCCGTCATCCACCACCTCTTCCACCCGTGCCACCCCAGCCCCTT
CTGCTCCAGCAGCTGCCTCAGCAACTAGCCCGAGCCCCGCGCCCAGTTCC
GGGAATGGAGCCAGCACAGCAGCCAGCCCGACCCAGCCCATCCAGCTGAG
CGACCTCCAGAGCATCCTGGCCACGATGAACGTACCAGCCGGGCCAGCAG
GCGGCCAGCAAGTGGACCTGGCCAGTGTGCTGACGCCGGAGATAATGGCT
CCCATCCTCGCCAACGCGGATGTCCAGGAGCGCCTGCTTCCCTACTTGCC
ATCTGGGGAGTCGCTGCCGCAGACCGCGGATGAGATCCAGAATACCCTGA
CCTCGCCCCAGTTCCAGCAGGCCCTGGGCATGTTCAGCGCAGCCTTGGCC
TCGGGGCAGCTGGGCCCCCTCATGTGCCAGTTCGGTCTGCCTGCAGAGGC
TGTGGAGGCCGCCAACAAGGGCGATGTGGAAGCGTTTGCCAAAGCCATGC
AGAACAACGCCAAGCCCGAGCAGAAAGAGGGCGACACGAAGGACAAGAAG
GACGAAGAGGAGGACATGAGCCTGGACTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMIPGVPQIDAESYILIDYNSGKVLAEQNAD
VRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSS
LMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYV
NALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKE
FTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLIS
AVMGGRTFKGREAESKKLLTWGFRFFETVNPLEVLFQGPGGSMTTSGALF
PSLVPGSRGASNKYLVEFRAGKMSLKGTTVTPDKRKGLVYIQQTDDSLIH
FCWKDRTSGNVEDDLIIFPDDCEFKRVPQCPSGRVYVLKFKAGSKRLFFW
MQEPKTDQDEEHCRKVNEYLNNPPMPGALGASGSSGHELSALGGEGGLQS
LLGNMSHSQLMQLIGPAGLGGLGGLGALTGPGLASLLGSSGPPGSSSSSS
SRSQSAAVTPSSTTSSTRATPAPSAPAAASATSPSPAPSSGNGASTAASP
TQPIQLSDLQSILATMNVPAGPAGGQQVDLASVLTPEIMAPILANADVQE
RLLPYLPSGESLPQTADEIQNTLTSPQFQQALGMFSAALASGQLGPLMCQ
FGLPAEAVEAANKGDVEAFAKAMQNNAKPEQKEGDTKDKKDEEEDMSLD*

• Vector Type: Bacterial
• DU Number: DU20192
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression vector for ADRM1 with N-terminal 6HIS-DAC tag (cleavage sites as follows: 6HIS-thrombin–DAC-Precission protease); Parental Plasmid 21713; Estimated MW of expressed protein : 73862 Daltons