cDNA Clone Ubiquitin

• Plasmid: pET156P-1-Ubiquitin G75A G76A dimer
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-Ubiquitin G75A G76A dimer
• Genbank: P62988*
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGGCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCTGCTTGATAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSGAMQIFVKTLTGKTITLEVEPSDTIEN
VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRA
AMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQ
LEDGRTLSDYNIQKESTLHLVLRLRAA**

• Vector Type: Bacterial
• DU Number: DU20197
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express linear ubiquitin dimer with N-terminal 6HIS tag (contains preScission protease site); Each unit differs from ref sequence in having C-terminal AA instead of GG. BamHI-NotI flanked dimer plus STOP codon. ; ; Estimated MW of expressed protein : 19741 Daltons