cDNA Clone Ubiquitin

• Plasmid: pET156P-1-Ubiquitin G75A G76A octamer
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-Ubiquitin G75A G76A octamer
• Genbank: P62988*
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGGCGCC ATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGT
GGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGG
AAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTG
GAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCT
GCACCTGGTCCTCCGTCTCAGAGCCGCCATGCAGATCTTCGTGAAGACCC
TGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAG
AATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCA
GAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTG
ACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGA
GCCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCT
CGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAG
ACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAA
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTC
CACCCTGCACCTGGTCCTCCGTCTCAGAGCCGCCATGCAGATCTTCGTGA
AGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACC
ATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGA
CCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCC
TGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGT
CTCAGAGCCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCAT
CACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGA
TCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCT
GGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAA
AGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCCGCCATGCAGATCT
TCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGT
GACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCC
TCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGAC
GCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTC
CTCCGTCTCAGAGCCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAA
GACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGG
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC
TTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACAT
CCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCCGCCATGC
AGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAG
CCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGG
CATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAG
ATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCAC
CTGGTCCTCCGTCTCAGAGCTGCTTGATAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSGAMQIFVKTLTGKTITLEVEPSDTIEN
VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRA
AMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQ
LEDGRTLSDYNIQKESTLHLVLRLRAAMQIFVKTLTGKTITLEVEPSDTI
ENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRL
RAAMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAG
KQLEDGRTLSDYNIQKESTLHLVLRLRAAMQIFVKTLTGKTITLEVEPSD
TIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVL
RLRAAMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
AGKQLEDGRTLSDYNIQKESTLHLVLRLRAAMQIFVKTLTGKTITLEVEP
SDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHL
VLRLRAAMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRL
IFAGKQLEDGRTLSDYNIQKESTLHLVLRLRAA**

• Vector Type: Bacterial
• Full Snapgene Map: pET156P-1-Ubiquitin G75A G76A octamer173.33 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU20199
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express ubiquitin octamer with N-terminal 6HIS tag (contains preScission protease site); Each unit differs from ref sequence in having C-terminal AA instead of GG. BamHI-NotI flanked octamer plus STOP codon. ; ; Estimated MW of expressed protein : 71190 Daltons
• Low Temp: No