cDNA Clone Ubiquitin

• Plasmid: pEGFPC4-Ubiquitin G75A G76A
• Parent Plasmid: pEGFPC4
• Expressed: eGFP-Ubiquitin G75A G76A
• Genbank: P62988
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGGCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCAT
CACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGA
TCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCT
GGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAA
AGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCTGCTTGATAAGCGG
CCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSPGSGAM
QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLE
DGRTLSDYNIQKESTLHLVLRLRAA**

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU20420
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Plasmid for mammalian expression of N-terminally eGFP tagged monoubiquitin ; Parental Plasmid DU32794; Estimated MW of expressed protein : 36387 Daltons