cDNA Clone Ubiquitin

• Plasmid: pcDNA5-FRT/TO-6His-6Gly-Ubiquitin (KallR mutant) + ATG
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: 6His-Ubiquitin (KallR)
• Genbank: P62988
• Tag: 6His
• Species: Human

Sequence of Insert

AAGCTTATGCATCATCACCATCACCATGGCGGAGGCGGAGGCGGATCCATGCAGATCTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAGGATCCAAGACAGGGAAGGC
ATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGA
TGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACC
TGGTCCTCCGTCTCAGAGGTGGGTGATAAGCGGCCGC

Amino Acid Sequence

MHHHHHHGGGGGGSMQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGI
PPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRGG**

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20447
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Flp-In vector for mammalian expression of N-terminally HIS tagged KallR ubiquitin (with initiating methionine); ; Estimated MW of expressed protein : 10144 Daltons