cDNA Clone UBE2T

• Plasmid: pGEX6P-1-UBE2T K8R K52R K156R
• Parent Plasmid: pGEX6P1
• Expressed: GST-UBE2T
• Genbank: NP_054895.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCAGAGAGCTTCACGTCTGAGGAGAGAGCTGCACATGTTAGC
CACAGAGCCACCCCCAGGCATCACATGTTGGCAAGATAAAGACCAAATGG
ATGACCTGCGAGCTCAAATATTAGGTGGAGCCAACACACCTTATGAGAAA
GGTGTTTTTAGGCTAGAAGTTATCATTCCTGAGAGGTACCCATTTGAACC
TCCTCAGATCCGATTTCTCACTCCAATTTATCATCCAAACATTGATTCTG
CTGGAAGGATTTGTCTGGATGTTCTCAAATTGCCACCAAAAGGTGCTTGG
AGACCATCCCTCAACATCGCAACTGTGTTGACCTCTATTCAGCTGCTCAT
GTCAGAACCCAACCCTGATGACCCGCTCATGGCTGACATATCCTCAGAAT
TTAAATATAATAAGCCAGCCTTCCTCAAGAATGCCAGACAGTGGACAGAG
AAGCATGCAAGACAGAAACAAAGGGCTGATGAGGAAGAGATGCTTGATAA
TCTACCAGAGGCTGGTGACTCCAGAGTACACAACTCAACACAGAAAAGGA
AGGCCAGTCAGCTAGTAGGCATAGAAAAGAAATTTCATCCTGATGTTTAG
GCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMQRASRLRRELHMLATEPP
PGITCWQDKDQMDDLRAQILGGANTPYEKGVFRLEVIIPERYPFEPPQIR
FLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPN
PDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQRADEEEMLDNLPEA
GDSRVHNSTQKRKASQLVGIEKKFHPDV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20485
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for GST-UBE2T K8R K52R K156R triple mutant.; ; Estimated MW of expressed protein : 49430 Daltons