cDNA Clone UBE2Q2

• Plasmid: pET28-UBE2Q2
• Parent Plasmid: pET28a original
• Expressed: 6His-UBE2Q2
• Genbank: AAH34342.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCGTC
CATCTTCGACAAGAACCACGAGCGATTCCGCATCGTCAGTTGGAAGCTGG
ACGAGCTGCACTGCCAGTTCCTGGTGCCGCAGCAGGGCAGCCCGCACTCG
CTGCCGCCGCCACTCACGCTCCACTGCAACATCACGGAATCCTATCCATC
TTCTTCACCGATATGGTTTGTGGATTCTGAAGACCCAAATCTGACATCAG
TTCTGGAACGTCTAGAAGATACTAAGAACAACAATTTGCTTCGTCAGCAA
TTGAAGTGGTTGATATGTGAACTCTGCAGTTTATATAACCTTCCTAAGCA
CCTGGATGTTGAGATGCTAGATCAACCACTACCCACGGGTCAGAATGGGA
CAACAGAAGAAGTGACTTCAGAAGAAGAGGAAGAAGAAGAAGAGATGGCT
GAAGATATAGAAGACTTAGATCACTATGAGATGAAGGAAGAAGAGCCTAT
TAGTGGGAAAAAGTCAGAGGATGAAGGAATTGAAAAAGAAAATTTGGCAA
TATTAGAGAAAATTAGGAAGACTCAAAGGCAAGACCATTTAAATGGTGCA
GTGTCTGGGTCAGTGCAAGCTTCAGATAGACTTATGAAAGAGCTCAGGGA
CATATACAGATCACAGAGTTATAAAACAGGGATTTATTCAGTGGAACTCA
TAAATGACAGTTTATATGACTGGCATGTTAAACTGCAGAAGGTTGACCCT
GATAGTCCTTTGCACAGTGATCTTCAGATCTTAAAAGAAAAAGAAGGCAT
AGAATATATTTTGCTTAACTTCTCTTTTAAGGATAACTTTCCATTTGATC
CTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGAGGGTATGTATTG
GGTGGAGGAGCATTATGTATGGAACTTCTCACAAAACAGGGCTGGAGCAG
TGCCTACTCAATAGAATCGGTCATCATGCAAATAAATGCCACCTTAGTCA
AAGGCAAAGCCAGAGTGCAGTTTGGAGCAAATAAGAATCAATATAATCTA
GCAAGAGCCCAACAATCCTATAATTCCATTGTACAGATACATGAGAAAAA
TGGCTGGTACACCCCTCCAAAGGAAGATGGCTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMSVSGLKAELKFLASI
FDKNHERFRIVSWKLDELHCQFLVPQQGSPHSLPPPLTLHCNITESYPSS
SPIWFVDSEDPNLTSVLERLEDTKNNNLLRQQLKWLICELCSLYNLPKHL
DVEMLDQPLPTGQNGTTEEVTSEEEEEEEEMAEDIEDLDHYEMKEEEPIS
GKKSEDEGIEKENLAILEKIRKTQRQDHLNGAVSGSVQASDRLMKELRDI
YRSQSYKTGIYSVELINDSLYDWHVKLQKVDPDSPLHSDLQILKEKEGIE
YILLNFSFKDNFPFDPPFVRVVLPVLSGGYVLGGGALCMELLTKQGWSSA
YSIESVIMQINATLVKGKARVQFGANKNQYNLARAQQSYNSIVQIHEKNG
WYTPPKEDG*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU20500
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express UBE2Q2 with N-terminal 6HIS tag (contains Thrombin cleavage site); ; Estimated MW of expressed protein : 46363 Daltons