cDNA Clone Ubiquitin

• Plasmid: pET156P-Ub1 FRET donor
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-LBT-Ubiquitin K48R K63R
• Genbank: P62988
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGTGCGCGGATTATAACAAAGATGGCTGGTATGAAGAACT
GGAGTGCGCGGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCA
TCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAG
ATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGC
TGGGAGACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGA
GAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGAGCGGCC
GC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMACADYNKDGWYEELECAAMQIFVKTL
TGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGRQLEDGRTLSD
YNIQRESTLHLVLRLRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20516
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express 6HIS-LBT(Lanthanide binding peptide)-Ubiquitin K48R K63R (contains preScission protease site); ; Estimated MW of expressed protein : 13230 Daltons