cDNA Clone USP5

• Plasmid: pET156P-N-terminal YFP Topaz FRET Acceptor
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-YFP Topaz ZnF UBP
• Genbank: P45974 U47927.1
• Tag: YFP
• Species: Human

Sequence of Insert

GGATCCAGCGAATTTGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCAC
CGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACA
AGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTG
ACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCAC
CCTCGTGACCACCTTCGGCTACGGCGTGCAGTGCTTCGCCCGCTACCCCG
ACCACATGAGGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTAC
GTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCG
CGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGA
AGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAG
TACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAA
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG
TGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCC
GTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCGCCCTGAGCAA
AGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCG
CCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGCTCGAAACAGAC
CAGACTAGTTTGTACAAAAAAGCAGGCACAGTCGACAAGCAGGAGGTGCA
GGCATGGGATGGGGAAGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCA
AGCAGTTGGACAACCCTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCC
AAGTGTGACATGAGAGAGAACCTGTGGCTCAACCTGACTGATGGCTCCAT
CCTCTGTGGGCGACGCTACTTCGATGGCAGTGGGGGCAACAACCACGCTG
TGGAGCACTACCGAGAGACAGGCTACCCGTTAGCTGTCAAGCTGGGCACC
ATCACCCCTGATGGAGCTGACGTGTACTCATATGATGAGGATGACATGGT
CCTGGACCCCAGCCTGGCTGAGCACCTGTCCCACTTCGGCATCGACATGC
TGAAGATGCAGAAGACAGACAAGTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSSEFATMVSKGEELFTGVVPILVELDGD
VNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGVQCF
ARYPDHMRQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVN
RIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNI
EDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYKLETDQTSLYKKAGTVDKQEVQAWDGEVRQVSKH
AFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGG
NNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHF
GIDMLKMQKTDK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20519
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express 6HIS-YFP Topaz-ZnF UBP (Ubiquitin Specific Protease Zinc Finger USP5) FRET Acceptor (contains preScission protease site); ; Estimated MW of expressed protein : 46292 Daltons