cDNA Clone USP5

• Plasmid: pET156P-C-terminal YFP Topaz FRET Acceptor
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-ZnF UBP-YFP Topaz
• Genbank: P45974 U47927.1
• Tag: YFP
• Species: Human

Sequence of Insert

GGATCCAGCGAATTTGCCACCAAGCAGGAGGTGCAGGCATGGGATGGGGA
AGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAGCAGTTGGACAACC
CTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGTGACATGAGA
GAGAACCTGTGGCTCAACCTGACTGATGGCTCCATCCTCTGTGGGCGACG
CTACTTCGATGGCAGTGGGGGCAACAACCACGCTGTGGAGCACTACCGAG
AGACAGGCTACCCGTTAGCTGTCAAGCTGGGCACCATCACCCCTGATGGA
GCTGACGTGTACTCATATGATGAGGATGACATGGTCCTGGACCCCAGCCT
GGCTGAGCACCTGTCCCACTTCGGCATCGACATGCTGAAGATGCAGAAGA
CAGACAAGCTCGAAACAGACCAGACTAGTTTGTACAAAAAAGCAGGCACA
GTCGACATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCAT
CCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCG
GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATC
TGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTT
CGGCTACGGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAGGCAGC
ACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACC
ATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTT
CGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCA
AGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGC
CACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAA
CTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACC
ACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGAC
AACCACTACCTGAGCTACCAGTCCGCCCTGAGCAAAGACCCCAACGAGAA
GCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTC
TCGGCATGGACGAGCTGTACAAGTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSSEFATKQEVQAWDGEVRQVSKHAFSLK
QLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAV
EHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDML
KMQKTDKLETDQTSLYKKAGTVDMVSKGEELFTGVVPILVELDGDVNGHK
FSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGVQCFARYPD
HMRQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELK
GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSV
QLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTA
AGITLGMDELYK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20520
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express 6HIS- ZnF UBP (Ubiquitin Specific Protease Zinc Finger USP5) -YFP Topaz- FRET Acceptor (contains preScission protease site); ; Estimated MW of expressed protein : 46292 Daltons