cDNA Clone UBE2Q2

• Plasmid: pGEX6P-UBE2Q2
• Parent Plasmid: pGEX6P1
• Expressed: GST-UBE2Q2
• Genbank: AAH34342.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCGTC
CATCTTCGACAAGAACCACGAGCGATTCCGCATCGTCAGTTGGAAGCTGG
ACGAGCTGCACTGCCAGTTCCTGGTGCCGCAGCAGGGCAGCCCGCACTCG
CTGCCGCCGCCACTCACGCTCCACTGCAACATCACGGAATCCTATCCATC
TTCTTCACCGATATGGTTTGTGGATTCTGAAGACCCAAATCTGACATCAG
TTCTGGAACGTCTAGAAGATACTAAGAACAACAATTTGCTTCGTCAGCAA
TTGAAGTGGTTGATATGTGAACTCTGCAGTTTATATAACCTTCCTAAGCA
CCTGGATGTTGAGATGCTAGATCAACCACTACCCACGGGTCAGAATGGGA
CAACAGAAGAAGTGACTTCAGAAGAAGAGGAAGAAGAAGAAGAGATGGCT
GAAGATATAGAAGACTTAGATCACTATGAGATGAAGGAAGAAGAGCCTAT
TAGTGGGAAAAAGTCAGAGGATGAAGGAATTGAAAAAGAAAATTTGGCAA
TATTAGAGAAAATTAGGAAGACTCAAAGGCAAGACCATTTAAATGGTGCA
GTGTCTGGGTCAGTGCAAGCTTCAGATAGACTTATGAAAGAGCTCAGGGA
CATATACAGATCACAGAGTTATAAAACAGGGATTTATTCAGTGGAACTCA
TAAATGACAGTTTATATGACTGGCATGTTAAACTGCAGAAGGTTGACCCT
GATAGTCCTTTGCACAGTGATCTTCAGATCTTAAAAGAAAAAGAAGGCAT
AGAATATATTTTGCTTAACTTCTCTTTTAAGGATAACTTTCCATTTGATC
CTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGAGGGTATGTATTG
GGTGGAGGAGCATTATGTATGGAACTTCTCACAAAACAGGGCTGGAGCAG
TGCCTACTCAATAGAATCGGTCATCATGCAAATAAATGCCACCTTAGTCA
AAGGCAAAGCCAGAGTGCAGTTTGGAGCAAATAAGAATCAATATAATCTA
GCAAGAGCCCAACAATCCTATAATTCCATTGTACAGATACATGAGAAAAA
TGGCTGGTACACCCCTCCAAAGGAAGATGGCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSVSGLKAELKFLASIFDK
NHERFRIVSWKLDELHCQFLVPQQGSPHSLPPPLTLHCNITESYPSSSPI
WFVDSEDPNLTSVLERLEDTKNNNLLRQQLKWLICELCSLYNLPKHLDVE
MLDQPLPTGQNGTTEEVTSEEEEEEEEMAEDIEDLDHYEMKEEEPISGKK
SEDEGIEKENLAILEKIRKTQRQDHLNGAVSGSVQASDRLMKELRDIYRS
QSYKTGIYSVELINDSLYDWHVKLQKVDPDSPLHSDLQILKEKEGIEYIL
LNFSFKDNFPFDPPFVRVVLPVLSGGYVLGGGALCMELLTKQGWSSAYSI
ESVIMQINATLVKGKARVQFGANKNQYNLARAQQSYNSIVQIHEKNGWYT
PPKEDG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20524
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for GST-UBE2Q2; ; Estimated MW of expressed protein : 69643 Daltons