cDNA Clone Ubiquitin

• Plasmid: pET28a-FLAG-Ubiquitin
• Parent Plasmid: pET28a original
• Expressed: FLAG-Ubiquitin
• Genbank: P62988
• Tag: FLAG
• Species: Human

Sequence of Insert

CCATGGACTACAAGGACGACGATGACAAGATGCAAATCTTCGTGAAGACC
CTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGA
GAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGC
AGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCT
GACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAG
AGGTGGGTGATAACTCGAGAGATCC

Amino Acid Sequence

MDYKDDDDKMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQ
RLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG**

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20637
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *6HIS and T7 tags removed by subcloning Nco1 BglII into Nco1 BamH1 when DU35145 was created; N terminal FLAG tag for bacterial expression of wild type ubiquitin