cDNA Clone Ubiquilin

• Plasmid: pTRE-Tight-maxFPGreen-SV5-TUBE
• Parent Plasmid: pTRE-Tight
• Expressed: maxFPGreen-SV5-TUBE
• Genbank: NM_053067.2
• Tag: SV5
• Species: Human

Sequence of Insert

GCTAGCGTTTAAACTTAAGCTTGCCACCATGGAGAGCGACGAGAGCGGCCTGCCCGCCATGGAGATCGAGTGCCGCATCACCGGCACCCTGAACGGCGTGGAGTTCGAGCTGGTGGGCGGCGGAGAGGGCACCCCCGAGCAGGGCCGCATGACCAACAAGATGAAGAGCACCAAAGGCGCCCTGACCTTCAGCCCCTACCTGCTGAGCCACGTGATGGGCTACGGCTTCTACCACTTCGGCACCTACCCCAGCGGCTACGAGAACCCCTTCCTGCACGCCATCAACAACGGCGGCTACACCAACACCCGCATCGAGAAGTACGAGGACGGCGGCGTGCTGCACGTGAGCTTCAGCTACCGCTACGAGGCCGGCCGCGTGATCGGCGACTTCAAGGTGATGGGCACCGGCTTCCCCGAGGACAGCGTGATCTTCACCGACAAGATCATCCGCAGCAACGCCACCGTGGAGCACCTGCACCCCATGGGCGATAACGATCTGGATGGCAGCTTCACCCGCACCTTCAGCCTGCGCGACGGCGGCTACTACAGCTCCGTGGTGGACAGCCACATGCACTTCAAGAGCGCCATCCACCCCAGCATCCTGCAGAACGGGGGCCCCATGTTCGCCTTCCGCCGCGTGGAGGAGGATCACAGCAACACCGAGCTGGGCATCGTGGAGTACCAGCACGCCTTCAAGACCCCGGATGCAGATGCCGGTGAAGAAGGTACCGAGCTCggatctGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGACAGCACCGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACCGTGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAAGGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACCGTGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAAGGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACCGTGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAAGGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACCGTGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAAGGTTACTGGGCTCCCAGCCATCATAGGGAGGTGGAggatccACTAGTCCAGTGTGGTGGAATTCTGCAGATATCCAGCACAGTGGCGGCCG
C

Amino Acid Sequence

MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKG
ALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYED
GGVLHVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLH
PMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
FRRVEEDHSNTELGIVEYQHAFKTPDADAGEEGTELGSGKPIPNPLLGLD
STGGGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAI
ERLLGSQPSGGGGSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQA
LIATGGDINAAIERLLGSQPSGGGGSGGGVNPQLQNPEVRFQQQLEQLSA
MGFLNREANLQALIATGGDINAAIERLLGSQPSGGGGSGGGVNPQLQNPE
VRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20645
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian response plasmid for expression of maxFPGreen-SV5-ubiquilin TUBE (for use with Clontech Advanced Tet-ON system) ; ; Estimated MW of expressed protein : 52763 Daltons