cDNA Clone USP9x

• Plasmid: pET156P-1-USP9x 1553-1995 wt (with D1635G)
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-USP9x 1553-1995
• Genbank: NP_001034680.2*
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCCTGGAAGTTCTGTTCCAGGGCCCGAAAGGATTCGTGGGGCTGAAAAATGCCGGTGCTACTTGTTACATGAATTCTGTGATTCAGCAACTCTACATGATTCCTTCCATTAGGAACGGTATTCTTGCCATTGAAGGCACAGGTAGTGATGTAGATGATGATATGTCTGGGGATGAGAAGCAGGACAATGAGAGCAATGTTGATCCCAGGGATGATGTATTTGGATATCCTCAACAATTTGAAGATAAACCAGCATTAAGTAAAACTGAAGGTAGAAAAGAGTACAACATTGGTGTCCTAAGACACCTTCAGGTCATCTTTGGTCATTTAGCTGCTTCTCGACTGCAATACTATGTGCCCAGAGGATTTTGGAAACAGTTCAGGCTTTGGGGTGAGCCTGTTAATCTGCGTGAACAACACGATGCTTTAGAATTTTTTAATTCATTGGTGGATAGTTTAGATGAAGCTTTAAAAGCTTTAGGACATCCAGCTATGCTAAGTAAAGTCTTAGGAGGTTCCTTTGCTGATCAGAAGATCTGCCAAGGCTGCCCACATAGGTACGAATGTGAAGAATCTTTTACGACCCTAAACGTAGACATTAGAAATCACCAAAATCTTCTTGATTCTTTGGAACAGTATGTCAAAGGAGATTTACTAGAAGGTGCAAATGCATATCATTGTGAAAAATGCAATAAAAAGGTTGATACCGTAAAGCGCTTGCTGATTAAAAAATTACCTCCTGTTCTTGCTATACAACTAAAGCGATTTGACTATGACTGGGAAAGAGAATGTGCAATCAAGTTCAATGATTATTTTGAATTTCCTCGAGAGCTGGACATGGAACCTTACACAGTTGCAGGTGTCGCAAAGCTGGAAGGGGATAATGTAAACCCAGAGAGTCAGTTGATACAACAGAGTGAGCAGTCTGAAAGTGAGACAGCAGGAAGCACAAAATACAGACTTGTGGGTGTGCTCGTACACAGTGGTCAAGCGAGTGGGGGGCATTATTATTCTTACATCATCCAAAGGAATGGTGGAGATGGTGAGAGAAATCGCTGGTATAAATTTGATGATGGTGATGTAACAGAATGTAAAATGGATGATGACGAAGAAATGAAAAACCAGTGTTTTGGTGGAGAGTACATGGGAGAAGTGTTTGATCACATGATGAAGCGTATGTCATACAGGCGCCAGAAAAGGTGGTGGAATGCTTATATACTTTTTTATGAACGAATGGACACAATAGACCAAGATGATGAGTTGATAAGATATATATCAGAGCTTGCTATCACCACCAGACCTCATCAGATTATTATGCCATCAGCCATTGAGAGAAGTGTACGGAAACAGAACTAG

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSLEVLFQGPKGFVGLKNAGATCYMNSVI
QQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESNVDPRDDVFGYPQ
QFEDKPALSKTEGRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFR
LWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQK
ICQGCPHRYECEESFTTLNVDIRNHQNLLDSLEQYVKGDLLEGANAYHCE
KCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPREL
DMEPYTVAGVAKLEGDNVNPESQLIQQSEQSESETAGSTKYRLVGVLVHS
GQASGGHYYSYIIQRNGGDGERNRWYKFDDGDVTECKMDDDEEMKNQCFG
GEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDTIDQDDELIRYISEL
AITTRPHQIIMPSAIERSVRKQN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20657
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Clone carries D1635G compared to updated reference sequence; Bacterial expression construct to express USP9x 1553-1995 WT (with D1635G) with N-terminal 6HIS tag (contains preScission protease site); ; Estimated MW of expressed protein : 54349 Daltons; ; All USP9X plasmids MUST be grown at 30 degrees C or less to prevent recombination.