cDNA Clone UBE2J1

• Plasmid: pET28a-UBE2J1 1-282
• Parent Plasmid: pET28a original
• Expressed: 6His-UBE2J1 1-282
• Genbank: NM_016021
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGAGACCCGCTACAACCTGAAGAGTCCGGCTGTTAAACGTTT
AATGAAAGAAGCGGCAGAATTGAAAGATCCAACAGATCATTACCATGCGC
AGCCTTTAGAGGATAACCTTTTTGAATGGCACTTCACGGTTAGAGGGCCC
CCAGACTCCGATTTTGATGGAGGAGTTTATCACGGGCGGATAGTACTGCC
ACCAGAGTATCCCATGAAACCACCAAGCATTATTCTCCTAACGGCTAATG
GTCGATTTGAAGTGGGCAAGAAAATCTGTTTGAGCATCTCAGGCCATCAT
CCTGAAACTTGGCAGCCTTCGTGGAGTATAAGGACAGCATTATTAGCCAT
CATTGGGTTTATGCCAACAAAAGGAGAGGGAGCCATAGGTTCTCTAGATT
ACACTCCTGAGGAAAGAAGAGCACTTGCCAAAAAATCACAAGATTTCTGT
TGTGAAGGATGTGGCTCTGCCATGAAGGATGTCCTGTTGCCTTTAAAATC
TGGAAGCGATTCAAGCCAAGCTGACCAAGAAGCCAAAGAACTGGCTAGGC
AAATAAGCTTTAAGGCAGAAGTCAATTCATCTGGAAAGACTATCTCTGAG
TCAGACTTAAACCACTCTTTTTCACTAACTGATTTACAAGATGATATACC
TACAACATTCCAGGGTGCTACGGCCAGTACATCGTACGGACTCCAGAATT
CCTCAGCAGCATCCTTTCATCAACCTACCCAACCTGTAGCTAAGAATACC
TCCATGAGCCCTCGACAGCGCCGGGCCCAGCAGCAGAGTCAGAGAAGGTT
GTCTACTTCACCAGATGTAATCCAGGGCCACCAGCCAAGAGACAACCACA
CTTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMETRYNLKSPAVKRLM
KEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPP
EYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAII
GFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSG
SDSSQADQEAKELARQISFKAEVNSSGKTISESDLNHSFSLTDLQDDIPT
TFQGATASTSYGLQNSSAASFHQPTQPVAKNTSMSPRQRRAQQQSQRRLS
TSPDVIQGHQPRDNHT*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU20686
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express UBE2J1 aa 1-1 282 with N-terminal 6HIS tag (contains Thrombin cleavage site); ; Estimated MW of expressed protein : 34709 Daltons