cDNA Clone WDR20

• Plasmid: pET156P-1-WDR20
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-WDR20
• Genbank: NM_144574.2
• Tag: 6His
• Species: Human

Sequence of Insert

GTCGACATGGCGACGGAGGGAGGAGGGAAGGAGATGAACGAGATTAAGAC
CCAATTCACCACCCGGGAAGGTCTGTACAAGCTGCTGCCGCACTCGGAGT
ACAGCCGGCCCAACCGGGTGCCCTTCAACTCGCAGGGATCCAACCCTGTC
CGCGTCTCCTTCGTAAACCTCAACGACCAGTCTGGCAACGGCGACCGCCT
CTGCTTCAATGTGGGCCGGGAGCTGTACTTCTATATCTACAAGGGGGTCC
GCAAGGCTGCTGACTTGAGTAAACCAATAGATAAAAGGATATACAAAGGA
ACACAGCCTACTTGTCATGACTTCAACCACCTAACAGCCACAGCAGAAAG
TGTCTCTCTCCTAGTGGGCTTTTCCGCAGGCCAAGTCCAGCTTATAGACC
CAATCAAAAAAGAAACTAGCAAACTTTTTAATGAGGAAAGACTAATAGAC
AAGTCACGAGTTACCTGTGTCAAATGGGTTCCCGGTTCGGAAAGCCTTTT
CCTAGTAGCCCACTCGAGTGGGAACATGTACTTATATAATGTGGAGCACA
CTTGTGGCACCACAGCCCCCCACTACCAGCTTCTGAAGCAGGGAGAGAGC
TTTGCCGTGCACACTTGCAAGAGCAAATCCACGAGGAACCCTCTCCTTAA
GTGGACGGTGGGCGAGGGGGCCCTCAACGAGTTTGCTTTCTCCCCAGATG
GCAAGTTCTTAGCGTGCGTGAGCCAGGACGGGTTTCTGCGGGTGTTCAAC
TTTGACTCAGTGGAGCTGCACGGTACGATGAAAAGCTACTTTGGGGGCTT
GCTGTGTGTGTGCTGGAGCCCGGATGGCAAGTACATCGTGACAGGTGGGG
AGGACGACTTGGTGACAGTCTGGTCCTTTGTAGACTGCCGAGTAATAGCC
AGAGGCCACGGGCACAAGTCCTGGGTCAGTGTTGTAGCGTTTGACCCTTA
TACCACTAGTGTAGAAGAAGGTGACCCTATGGAGTTTAGTGGCAGCGATG
AGGACTTCCAAGACCTTCTTCATTTTGGCAGAGATCGAGCAAATAGTACA
CAGTCCAGGCTCTCCAAACGGAACTCTACAGACAGCCGCCCCGTAAGTGT
CACGTATCGGTTTGGTTCCGTGGGCCAGGACACACAGCTCTGTTTATGGG
ACCTTACAGAAGATATCCTTTTCCCTCACCAACCCCTCTCAAGAGCAAGG
ACACACACAAATGTCATGAATGCCACGAGTCCTCCTGCTGGAAGCAATGG
GAACAGTGTTACAACACCCGGGAACTCTGTGCCGCCTCCTCTGCCACGGT
CCAACAGCCTTCCACATTCAGCAGTCTCAAATGCTGGCAGCAAAAGCAGT
GTCATGGACGGGGCCATTGCTTCTGGGGTCAGCAAATTTGCAACACTTTC
ACTACATGACCGGAAGGAGAGGCACCACGAGAAAGATCACAAGCGAAATC
ATAGCATGGGACACATTTCTAGCAAGAGCAGTGACAAACTGAATCTAGTT
ACCAAAACCAAAACGGACCCTGCTAAAACTCTGGGAACGCCCCTGTGTCC
TCGAATGGAAGATGTTCCCTTGTTAGAGCCGCTGATATGTAAAAAGATAG
CACATGAGAGACTGACTGTACTAATATTTCTTGAAGACTGTATAGTCACT
GCTTGTCAGGAGGGATTTATTTGCACATGGGGAAGGCCTGGTAAAGTGGT
AAGTTTTAATCCTTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSPEFPGVDMATEGGGKEMNEIKTQFTTR
EGLYKLLPHSEYSRPNRVPFNSQGSNPVRVSFVNLNDQSGNGDRLCFNVG
RELYFYIYKGVRKAADLSKPIDKRIYKGTQPTCHDFNHLTATAESVSLLV
GFSAGQVQLIDPIKKETSKLFNEERLIDKSRVTCVKWVPGSESLFLVAHS
SGNMYLYNVEHTCGTTAPHYQLLKQGESFAVHTCKSKSTRNPLLKWTVGE
GALNEFAFSPDGKFLACVSQDGFLRVFNFDSVELHGTMKSYFGGLLCVCW
SPDGKYIVTGGEDDLVTVWSFVDCRVIARGHGHKSWVSVVAFDPYTTSVE
EGDPMEFSGSDEDFQDLLHFGRDRANSTQSRLSKRNSTDSRPVSVTYRFG
SVGQDTQLCLWDLTEDILFPHQPLSRARTHTNVMNATSPPAGSNGNSVTT
PGNSVPPPLPRSNSLPHSAVSNAGSKSSVMDGAIASGVSKFATLSLHDRK
ERHHEKDHKRNHSMGHISSKSSDKLNLVTKTKTDPAKTLGTPLCPRMEDV
PLLEPLICKKIAHERLTVLIFLEDCIVTACQEGFICTWGRPGKVVSFNP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20710
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express WDR20 with N-terminal 6HIS tag (contains preScission protease site); ; Estimated MW of expressed protein : 66080 Daltons