cDNA Clone Ubiquitin

• Synonyms: -
• Plasmid: pGEX6P1-Ubiquitin dimer
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3-Ubiquitin dimer
• Genbank: P62988
• Tag: GST
• Species: Human

Sequence of Insert

GCCGGCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCT
CGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAG
ACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAA
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTC
CACCCTGCACCTGGTCCTCCGTCTCAGAGGCGCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAGMQIFVKTLTGKTITLEV
EPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTL
HLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQ
RLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG**

• Vector Type: Bacterial
• Antibiotic: Amp; Kan
• Full Snapgene Map: pGEX6P1-Ubiquitin dimer44.57 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU20729
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GST-ubiquitin (wild type) dimer and intermediate in the assembly of wild type polyubiquitin chains; ; Estimated MW of expressed protein : 44064 Daltons
• Low Temp: No