cDNA Clone Ubiquilin

• Plasmid: pGEX-TEV-6His-ubiquilin TUBE
• Parent Plasmid: pGEX TEV 6HIS
• Expressed: GST-TEV-6His-TUBE
• Genbank: NM_053067.2
• Tag: GST
• Tag: 6His
• Species: Human

Sequence of Insert

AGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATT
TCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACCGTGAAG
CAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATT
GAAAGGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGG
AGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATTTCAGCAACAACTGG
AACAACTCAGTGCAATGGGATTTTTGAACCGTGAAGCAAACTTGCAAGCT
CTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAAGGTTACTGGG
CTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGC
TACAGAATCCAGAAGTCAGATTTCAGCAACAACTGGAACAACTCAGTGCA
ATGGGATTTTTGAACCGTGAAGCAAACTTGCAAGCTCTAATAGCAACAGG
AGGTGATATCAATGCAGCTATTGAAAGGTTACTGGGCTCCCAGCCATCAG
GAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAA
GTCAGATTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAA
CCGTGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATG
CAGCTATTGAAAGGTTACTGGGCTCCCAGCCATCATAGGGAGGTGGAGGA
TCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGHHHHHHGSGGGVNPQLQNPEVRF
QQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPSGGGGSGGG
VNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLG
SQPSGGGGSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATG
GDINAAIERLLGSQPSGGGGSGGGVNPQLQNPEVRFQQQLEQLSAMGFLN
REANLQALIATGGDINAAIERLLGSQPS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20866
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid for GST-(TEV)-6HIS-ubiquilin TUBE concatamer (4 x UBA domain). Bacterial expression plasmid for GST-(TEV)-6HIS-ubiquilin TUBE concatamer (4 x UBA domain). Parental SC20856 (pGEX-TEV-6HIS)