cDNA Clone APP BP1

• Synonyms: NAE1
• Plasmid: pFastBacDual DAC_TEV-APP BP1, UBA3 (untagged)
• Parent Plasmid: pFastbacDual DAC TEV
• Expressed: DAC-APP BP1
• Genbank: NP_003896
• Tag: DAC
• Species: Human

Sequence of Insert

GGATCCATGGCGCAGCTGGGAAAGCTGCTCAAGGAGCAGAAGTACGACCGGCAGCTGAGGTTGTGGGGTGATCATGGGCAAGAGGCTTTAGAATCTGCTCATGTTTGCCTAATAAATGCAACAGCCACAGGAACTGAAATTCTTAAAAACTTGGTACTACCAGGTATTGGTTCGTTTACAATTATTGATGGAAATCAGGTCAGCGGAGAAGATGCTGGAAACAATTTCTTCCTTCAAAGAAGCAGTATCGGCAAGAACCGAGCTGAAGCTGCCATGGAATTCTTACAAGAATTAAATAGCGATGTCTCTGGAAGTTTTGTGGAAGAGAGTCCAGAAAACCTTCTAGACAATGATCCCTCATTTTTCTGTAGGTTTACTGTTGTAGTTGCAACTCAGCTTCCTGAAAGCACTTCACTACGCTTAGCAGATGTCCTCTGGAATTCCCAGATTCCTCTTTTGATCTGTAGGACATATGGACTAGTTGGTTATATGAGGATCATTATAAAAGAACATCCAGTAATAGAATCTCATCCAGATAATGCATTAGAGGATCTACGACTAGATAAGCCATTTCCTGAACTGAGAGAACATTTTCAGTCCTATGATTTGGATCATATGGAAAAAAAGGACCACAGTCATACTCCATGGATTGTGATCATAGCTAAATATTTAGCACAGTGGTATAGTGAAACAAATGGACGAATACCTAAAACGTATAAAGAAAAAGAGGACTTCAGAGATTTGATTAGACAAGGAATTCTAAAAAATGAAAATGGGGCTCCAGAAGATGAAGAGAATTTTGAAGAAGCTATTAAAAATGTGAACACAGCACTAAATACAACTCAGATCCCAAGCAGTATTGAAGATATATTTAATGATGATCGCTGCATAAATATCACCAAACAGACTCCATCATTTTGGATTTTAGCTCGTGCCTTAAAGGAATTTGTGGCCAAAGAGGGTCAAGGAAATTTACCTGTTCGAGGCACAATTCCTGATATGATTGCAGATTCAGGCAAATATATAAAACTGCAAAACGTTTACCGTGAAAAAGCAAAGAAAGATGCTGCCGCTGTGGGTAATCATGTTGCCAAATTGCTGCAGTCCATTGGCCAGGCACCAGAGTCCATTTCAGAGAAAGAATTAAAATTACTCTGCAGCAATTCTGCATTTCTTCGAGTGGTAAGATGTCGATCCTTAGCTGAAGAATATGGTTTGGATACAATTAACAAGGATGAAATTATTTCTAGCATGGACAATCCAGATAATGAAATAGTGTTGTACTTAATGTTACGGGCTGTTGATAGATTTCATAAACAACAGGGTAGATATCCAGGAGTATCTAACTATCAAGTTGAAGAAGATATAGGAAAGTTGAAGTCTTGTCTCACTGGCTTCCTTCAGGAATATGGTTTATCTGTAATGGTGAAAGATGATTATGTCCACGAATTTTGCCGATATGGAGCTGCTGAGCCACATACCATTGCTGCATTCTTGGGGGGAGCTGCTGCTCAAGAGGTCATCAAAATAATCACCAAACAATTTGTAATTTTTAATAATACTTACATTTACAGTGGCATGTCACAAACTTCAGCAACTTTCCAGTTGTAGAAGCTT

Amino Acid Sequence

MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNPLVPRGSENLYFQGGSMAQLGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL*KL

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU21784
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: vector has thrombin site upstream of TEV