cDNA Clone ZNRF2 (genomic)

• Plasmid: pAAV + SEPT + ZNRF2 targeting construct
• Parent Plasmid: pAAV MCS
• Genbank: NM_147128.3 GI:40316919
• Species: Human

Sequence of Insert

gcggccgcCATTTTTATATGTGCAATTTAATAGCATTTAAGACATTCACAACCCCCATCTTTATCTGGTTCTAAACACTTCATCGCCCCAAAAGAAAACCTCTGGTGTGATTCCACTTACATGAGGTAGCTAAAATACTCAAACTCATAGAAGCAGAGAGTAGACTAGTGGTGGCCAGGGGCTGGGCGGGGGGAAGGGGAGTGGTGGATTAATGGGTATAACATTTTAGTTATGCACGATGAGTAAGTTCTAGACATCTGCCATACAACATAGTGCCTACAGTTGACAATACTATaTTGTACACTTGAAGATTTGTTAAGAGGATAGAGCATATGTTAGTCTTACCACAATAAAATAAAGTTTTTAAAAGGGGAAAAAACGGGTCAGGCGTGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGTGGATCACGAGGTCAGGAGTTCGAGACCAGCCCGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAACTAGCCAGGCGTGGTGGTGGGCGCCTGTAATCCTAGCTACTCGGGAGGCTGAGACAGGAAAATCACTTGAACCCAGGAGGCGGGGGTTGCAGTGAGCCGAGATAGTGCCACTGCACTCCAGTCTGGGTGACAGAGTGAGACTCCATCTCAAATAAAAGCGGGGTGGTGGGGGGGGAGAAAACCCCCTACCCATTAAGCAGTCAtTCCCCATATCCCTCTGTCCCCAATACTGATCTCCTTTCCCTCTCTATGGATGTATCTATTCTGGACATTTCATATAAATGGGTTCATACAATAGGTGGTTTCTTTTACTTTGTGTAGTATTTTTGACGTTTATCCATGTTTTAGCATGTGGTGGTTCTTCCTTTTCTTGGCTTAGTAATATTCCATTGTGTGGATACATCATCTTTTGTTTATCCGTTGCGCAGTTTGTAGACACTGGGTTGTTTCCACCTTTGGGCTGTTGTGAACAATGCTGCTGTGAACCTCCGTGCACAAGGATTTTCTTGGGGACCTGTTTCTTCCTCTCATTCTTGCCTCCTACCACTCTACCCATTACCATATTTTTCCAAACCAGACATCAGCACACTGGCTACGTGTGTGTTTAGGAAAGCATCTGTGGAGGGAAACAGCTTGCCTCATGCTGCCCCTGCTTCCTGTCTACCCTTCTCCAAGCCTTTTTCGTCCGCTGTCACCgagctccaccgcggtggcggccgctctagaactagtggatccataacttcgtatagcatacattatacgaagttatggaattaattcgctgtctgcgagggccagctgttggggtgagtactccctctcaaaagcgggcatgacttctgcgctaagattgtcagtttccaaaaacgaggaggatttgatattcacctggcccgcggtgatgcctttgagggtggccgcgtccatctggtcagaaaagacaatctttttgttgtcaagcttgaggtgtggcaggcttgagatctggccatacacttgagtgacaatgacatccactttgcctttctctccacaggtgtccactcccaggtccaactgcaggtcgagcgtcgagaacgttactggccgaagccgcttggaataaggccggtgtgcgtttgtctatatgttattttccaccatattgccgtcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggggtctttcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagttcctctggaagcttcttgaagacaaacaacgtctgtagcgaccctttgcaggcagcggaaccccccacctggcgacaggtgcctctgcggccaaaagccacgtgtataagatacacctgcaaaggcggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatggctctcctcaagcgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtatgggatctgatctggggcctcggtgcacatgctttacatgtgtttagtcgaggttaaaaaaacgtctaggccccccgaaccacggggacgtggttttcctttgaaaaacacgatgataagcttgccacaaccatggctgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgggggatccagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttctagggggccccggcgaggccgggatgagttgggaataacttcgtatagcatacattatacgaagttatccaaattttaaggtaccactgtgcatatgtatgaattcGGGAGCTGATGGGGAGACCCGTAGAGCCTCACAGGTTGGAGGAGCTTCGGATCCCACTGGGAAGGTTGGTTCTCCGTCTGTCTCCCTGCCTCTTCTTCCTCTACGGGTCCCTCTGCTCCACAGGGGTAGAACATCAATCTGTGCGAGGAAGGCCAGGCGGAGGGTGTACCCACTGCCTTGCACTGGCCTTCTCCCTAGAGGGCCGGGAGGCAGGAAGAGCCATTTCCTGTGGGGCCACAGCACTGGGCACAGTTAAAAGTAGCAGGGCCCAGATATGCCTTGGGACTCCAGTGTGAGCCTCGTCCTTGTTTCCAGCTGGAAGGAAGGCACCCTCTTGCCCAAGACAGGACACTGTGCTGCCTGGGGCCAGCACCTGcTGAATCCTCGAGCCCAGAGCTTCTTCCTGACACTCAGGCCTTTCAAAGCCTGACTCTCAGGGCCACTGCTGCACAGGGTGACAACCGGGCATGGCACGCAGTGTGGCTGGTGCAGAGGAGGCTTAAGGCTGGCCTGGGAGAGATTCTTGGTCTAGCTCCCAGCACGGAGCCTTGCTTGTGTCCCAGGGCGGCACTAACCAGAACCAGAAAAGGAAATCTTTGGCCCTCGGGGAGAATTATGCCAATTATCTGTCTGGTTCCTGTCTTTGAAAATGCCCAGATGTTGGTTTACAAATCAGGAAGCTGTCTTGGGGCAATCTCTGTTCCCTCTTTGATGCAGAGAGCCTTTCTCTGTAAACACTGGAGAGCAGGGCTTCACTGTCTGGCTCCCCTCCCGCCTCTTGCAGGAAGGACTAGATGCTGCTGATCAGCTGATCATGGCTGAGCCGGGTAGAGCTTGGGGCTCTCCTAAGAGGTCACCCACTGGGAGGGAGCTTCACAGCTGTTCTTTTGACCCAGGAAGGAGCTCTTTCAGGACTGTGGCACCCAGAGCCGTGGCCCTCCTGCCATGCCATTGTTCCCACTTCCCTGCCCTTGGGAGTCTTGGAGACAGCTGACTAGCTGCCCTGAGGGGTCCACCGCTCTCACCTTCTCACTGGTGGCTGCTGCCATTCTCCTGGGCTCTGCACAGAAAGCTTTGGTCCTTCTGAAAGCCACTCTGCCCCTTCCTGCAGCACCAGATCCCCCTGAGCCGGTACTGCTGCCTCCACCTGGGCCAAGACTAGTCgcggccgc

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU40015
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: For knock-out of ZNRF2 using Bunz method.; ; All ZNRF2 plasmids MUST be grown at 30 degrees C or less to prevent recombination.