cDNA Clone
Ubiquitin

Plasmid
pET24a-Ubiquitin-SATGSHGHSHAG
Parent Plasmid
pET24a original
Expressed
Ubiquitin-SATGSHGHSHAG
Genbank
P62988*
Tag
HA
Species
Human
Sequence of Insert
CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAtctgctacaggctctcatggccattcccatgccggttgataa
Amino Acid Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRSATGSHGHSHAG**
Vector Type
Bacterial
Antibiotic
Kan
DU Number
DU21978
Location
PPU
MTA
Notes
Differs from reference sequence in having silent mutation at Arg73 (a220c substitution). Also C-terminal GG removed from ubiquitin. T7 tag in pET24a was removed by subcloning into the Nde1 site, to give untagged protein im the original template construct SC20027.
Price
£125.00
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