cDNA Clone UNC13B

• Plasmid: pcDNA5-FRT/TO-GFP UNC13B (AAI11782)
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP UNC13B
• Genbank: AAI11782
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCAATGTCACTGCTCTGCGTGCGCGTTAAAAGGGCCAAATTCCA
GGGTTCACCAGATAAATTTAACACATATGTGACCCTGAAAGTACAGAATG
TGAAGAGCACAACTGTAGCAGTTCGTGGTGATCAGCCTTCCTGGGAACAG
GATTTCATGTTTGAGATTAGTCGCCTGGACCTGGGTCTAAGTGTGGAGGT
ATGGAACAAAGGACTGATCTGGGACACCATGGTGGGGACTGTGTGGATTG
CGCTGAAGACTATTCGTCAGTCGGATGAGGAAGGGCCTGGGGAATGGTCC
ACATTAGAGGCAGAGACGTTAATGAAAGACGATGAGATCTGTGGAACTAG
AAACCCAACTCCTCATAAAATTTTGCTTGATACAAGATTTGAGTTGCCTT
TTGATATCCCAGAGGAGGAAGCCAGATATTGGACCTACAAATGGGAGCAA
ATCAATGCCTTGGGAGCTGACAATGAGTATTCTAGTCAAGAAGAAAGCCA
GAGGAAGCCATTGCCCACTGCTGCCGCCCAGTGTTCTTTTGAAGACCCTG
ATAGTGCCGTCGATGACCGAGATAGTGACTATCGCAGTGAGACCAGCAAC
AGCTTCCCACCTCCTTACCATACAGCTTCCCAGCCCAACGCTTCTGTGCA
CCAGTTCCCTGTGCCGGTGCGATCGCCACAGCAGCTGCTACTTCAAGGCA
GTTCCCGGGACTCTTGTAATGACTCTATGCAAAGTTATGACCTTGATTAT
CCAGAGCGGCGGGCTATCAGCCCCACCAGCAGCAGTAGGTATGGCTCCTC
CTGTAATGTGAGTCAAGGAAGCTCTCAGCTAAGTGAACTAGACCAGTATC
ACGAACAAGATGACGACCATCGGGAGACGGACTCGATTCATTCTTGCCAC
AGCTCTCACAGCCTGTCCAGAGATGGCCAAGCAGGTTTTGGAGAACAAGA
GAAACCCTTGGAGGTGACAGGTCAAGCAGAGAAGGAGGCAGCATGTGAAC
CCAAGGAGATGAAAGAAGATGCCACAACCCACCCTCCCCCAGATCTGGTG
CTGCAAAAAGACCACTTCCTAGGTCCCCAGGAGAGTTTTCCTGAGGAGAA
TGCATCTTCACCATTTACCCAAGCCAGAGCACATTGGATCCGAGCAGTTA
CCAAGGTTCGACTCCAGCTGCAGGAGATTCCAGATGATGGTGACCCCTCT
CTGCCTCAGTGGCTCCCGGAAGGGCCAGCCGGAGGGCTCTATGGCATTGA
CAGCATGCCAGATTTACGCAGAAAGAAGCCACTGCCACTTGTCAGTGATC
TGTCACTGGTCCAGTCTCGGAAGGCAGGAATCACTTCTGCAATGGCTACA
CGCACTTCTCTTAAGGACGAAGAGCTGAAATCCCACGTGTATAAGAAAAC
CCTGCAGGCCTTAATCTACCCCATTTCGTGCACCACTCCTCATAACTTTG
AGGTCTGGACGGCCACTACCCCAACCTACTGCTATGAGTGTGAAGGCCTG
CTCTGGGGCATTGCCCGGCAGGGCATGCGCTGCAGCGAATGTGGAGTCAA
GTGCCATGAGAAGTGCCAGGATCTGCTCAATGCTGACTGCCTGCAGCGGG
CTGCAGAAAAGAGCTGTAAACATGGAGCTGAGGACCGGACCCAGAACATT
ATCATGGCCATGAAGGACCGCATGAAGATCCGAGAGCGAAATAAGCCAGA
GATCTTTGAAGTTATCCGGGACGTCTTCACAGTGAACAAAGCTGCCCATG
TGCAGCAGATGAAAACAGTGAAGCAGAGTGTACTGGATGGCACCTCCAAA
TGGTCAGCCAAGATCACCATTACTGTGGTGTGTGCCCAGGGCCTACAAGC
CAAGGACAAAACAGGATCCAGTGACCCTTACGTGACTGTGCAAGTCAGCA
AAACTAAGAAGCGTACCAAGACCATTTTTGGAAATTTGAATCCTGTTTGG
GAGGAGAAGTTCCATTTTGAGTGCCACAACTCCTCTGACCGCATTAAGGT
GCGTGTATGGGATGAGGATGATGACATCAAGTCAAGAGTAAAGCAACGCC
TAAAGCGAGAGTCTGATGATTTCCTTGGCCAAACCATCATTGAGGTTCGG
ACCCTAAGTGGCGAGATGGACGTCTGGTACAACTTGGAGAAGAGGACAGA
CAAATCAGCCGTCTCGGGGGCTATCCGACTACAAATCAGTGTGGAGATCA
AGGGGGAGGAGAAAGTAGCCCCATACCACGTGCAGTATACATGTCTCCAT
GAGAATCTTTTCCATTACCTCACAGACATTCAGGGCAGTGGAGGAGTCCG
CATCCCTGAAGCTCGAGGAGACGATGCCTGGAAGGTGTACTTTGATGAGA
CAGCCCAAGAAATTGTGGATGAATTTGCCATGCGTTATGGCATTGAGTCC
ATATATCAGGCCATGACGCACTTTGCATGTTTATCATCCAAGTACATGTG
TCCTGGTGTGCCAGCAGTGATGAGCACCTTACTGGCCAACATCAACGCCT
ACTATGCCCACACAACTGCCTCTACCAATGTCTCTGCATCTGATCGCTTT
GCAGCCTCCAACTTTGGGAAAGAGAGATTTGTAAAACTGCTGGACCAGCT
ACACAACTCACTGAGGATCGACCTCTCTACATACAGGAATAATTTCCCTG
CTGGGAGTCCTGAACGGCTTCAGGACTTAAAATCCACAGTGGATTTGCTG
ACCAGCATTACTTTCTTCAGAATGAAGGTACAAGAACTGCAAAGCCCTCC
AAGAGCCAGCCAGGTGGTAAAGGATTGTGTGAAGGCCTGTTTGAACTCCA
CATATGAATATATCTTCAACAACTGCCACGACTTATACAGCCGCCAGTAC
CAGCTGAAGCAGGAGCTACCTCCAGAGGAACAAGGGCCCAGCATTCGGAA
CCTGGATTTCTGGCCCAAGCTCATCACACTCATCGTGTCAATCATAGAGG
AAGATAAGAATTCCTACACACCTGTTCTGAACCAGTTTCCTCAGGAGTTG
AATGTGGGAAAAGTCAGCGCAGAAGTGATGTGGCATTTGTTTGCCCAAGA
CATGAAATATGCATTGGAGGAGCATGAGAAAGACCACCTGTGTAAAAGTG
CTGACTACATGAACCTGCACTTCAAGGTGAAGTGGCTCCACAATGAATAC
GTGCGGGATCTGCCTGTCCTCCAGGGGCAGGTGCCTGAGTACCCAGCGTG
GTTTGAGCAGTTCGTGCTACAATGGCTGGATGAGAATGAGGATGTATCCC
TGGAATTCCTGCGTGGGGCCCTGGAACGAGATAAGAAGGATGGATTCCAG
CAGACATCAGAGCATGCACTCTTTTCCTGCTCTGTGGTGGATGTCTTCAC
ACAACTCAATCAGAGCTTTGAGATCATCCGGAAGCTGGAATGCCCAGACC
CCAGCATCCTTGCCCACTACATGAGGAGGTTTGCTAAGACCATCGGGAAG
GTGCTGATGCAGTATGCAGACATCTTGTCAAAGGACTTCCCAGCCTATTG
CACAAAGGAGAAACTGCCCTGCATCCTGATGAACAACGTGCAGCAACTGA
GGGTCCAGCTGGAGAAAATGTTTGAGGCCATGGGAGGCAAGGAGCTGGAC
CTTGAAGCTGCAGACAGTCTGAAGGAGCTGCAGGTGAAACTGAATACGAT
TCTGGATGAGCTCAGCATGGTGTTTGGAAACAGTTTCCAGGTACGGATTG
ATGAGTGTGTTCGACAAATGGCCGACATCCTGGGCCAGGTTCGGGGCACA
GGGAATGCATCTCCAGACGCCAGGGCCTCAGCGGCTCAGGATGCAGATAG
CGTACTCCGGCCTCTCATGGACTTCCTGGATGGCAACCTCACCCTCTTTG
CCACTGTGTGTGAGAAGACGGTTCTGAAGCGTGTACTGAAGGAGCTCTGG
CGCGTGGTGATGAACACAATGGAGAGGATGATTGTTCTGCCCCCACTCAC
TGACCAGACGGGCACCCAGCTGATCTTCACTGCTGCCAAGGAGCTGAGCC
ATCTTTCCAAACTCAAGGATCACATGGTACGAGAGGAAACACGGAATCTC
ACTCCAAAGCAGTGTGCAGTCCTTGACCTCGCCCTGGACACCATCAAGCA
ATACTTCCATGCAGGAGGCAATGGGCTGAAGAAAACCTTCCTGGAGAAGA
GCCCAGATCTGCAGTCTCTACGCTATGCCCTGTCTCTGTACACACAGACT
ACTGACACTCTCATCAAGACCTTTGTGCGCTCGCAGACCACCCAAGTGCA
TGATGGGAAAGGTATTAGATTTACTGCTAATGAGGACATTCGTCCGGAAA
AGGGGTCTGGTGTGGACGATCCTGTGGGAGAAGTCTCTATTCAGGTGGAC
TTGTTTACACACCCTGGTACTGGGGAGCACAAGGTCACAGTGAAAGTGGT
GGCTGCCAATGACCTCAAGTGGCAGACAGCGGGTATGTTCCGGCCTTTCG
TGGAGGTGACTATGGTTGGCCCACACCAAAGTGATAAGAAGAGGAAGTTC
ACAACCAAATCCAAAAGCAACAACTGGGCCCCCAAGTACAATGAGACATT
CCACTTCCTCCTGGGAAATGAGGAGGGGCCCGAGTCCTATGAGTTGCAGA
TATGCGTGAAGGATTACTGCTTTGCCCGGGAAGATCGCGTGCTAGGGCTG
GCTGTGATGCCTCTGAGGGATGTCACAGCCAAGGGCAGCTGTGCCTGCTG
GTGCCCCTTGGGCCGGAAGATCCATATGGATGAGACAGGCCTGACCATTC
TCCGGATTTTATCTCAGAGGAGCAATGACGAGGTGGCCCGAGAATTTGTG
AAACTCAAATCAGAGTCTCGTTCCACGGAGGAGGGGAGCTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTI
DAAAMSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWE
QDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEW
STLEAETLMKDDEICGTRNPTPHKILLDTRFELPFDIPEEEARYWTYKWE
QINALGADNEYSSQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETS
NSFPPPYHTASQPNASVHQFPVPVRSPQQLLLQGSSRDSCNDSMQSYDLD
YPERRAISPTSSSRYGSSCNVSQGSSQLSELDQYHEQDDDHRETDSIHSC
HSSHSLSRDGQAGFGEQEKPLEVTGQAEKEAACEPKEMKEDATTHPPPDL
VLQKDHFLGPQESFPEENASSPFTQARAHWIRAVTKVRLQLQEIPDDGDP
SLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMA
TRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEG
LLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSCKHGAEDRTQN
IIMAMKDRMKIRERNKPEIFEVIRDVFTVNKAAHVQQMKTVKQSVLDGTS
KWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVSKTKKRTKTIFGNLNPV
WEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEV
RTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCL
HENLFHYLTDIQGSGGVRIPEARGDDAWKVYFDETAQEIVDEFAMRYGIE
SIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDR
FAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDL
LTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQ
YQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLNQFPQE
LNVGKVSAEVMWHLFAQDMKYALEEHEKDHLCKSADYMNLHFKVKWLHNE
YVRDLPVLQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGF
QQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPSILAHYMRRFAKTIG
KVLMQYADILSKDFPAYCTKEKLPCILMNNVQQLRVQLEKMFEAMGGKEL
DLEAADSLKELQVKLNTILDELSMVFGNSFQVRIDECVRQMADILGQVRG
TGNASPDARASAAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKEL
WRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRN
LTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQ
TTDTLIKTFVRSQTTQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQV
DLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRK
FTTKSKSNNWAPKYNETFHFLLGNEEGPESYELQICVKDYCFAREDRVLG
LAVMPLRDVTAKGSCACWCPLGRKIHMDETGLTILRILSQRSNDEVAREF
VKLKSESRSTEEGS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16715
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee