cDNA Clone UNC13B

• Plasmid: pcDNA5-FRT/TO-GFP-TAP UNC13B (AAI11782)
• Parent Plasmid: pcDNA5 FRT/TO GFPTAP
• Expressed: GFPTAP UNC13B
• Genbank: AAI11782
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

GCGGCCGCAATGTCACTGCTCTGCGTGCGCGTTAAAAGGGCCAAATTCCA
GGGTTCACCAGATAAATTTAACACATATGTGACCCTGAAAGTACAGAATG
TGAAGAGCACAACTGTAGCAGTTCGTGGTGATCAGCCTTCCTGGGAACAG
GATTTCATGTTTGAGATTAGTCGCCTGGACCTGGGTCTAAGTGTGGAGGT
ATGGAACAAAGGACTGATCTGGGACACCATGGTGGGGACTGTGTGGATTG
CGCTGAAGACTATTCGTCAGTCGGATGAGGAAGGGCCTGGGGAATGGTCC
ACATTAGAGGCAGAGACGTTAATGAAAGACGATGAGATCTGTGGAACTAG
AAACCCAACTCCTCATAAAATTTTGCTTGATACAAGATTTGAGTTGCCTT
TTGATATCCCAGAGGAGGAAGCCAGATATTGGACCTACAAATGGGAGCAA
ATCAATGCCTTGGGAGCTGACAATGAGTATTCTAGTCAAGAAGAAAGCCA
GAGGAAGCCATTGCCCACTGCTGCCGCCCAGTGTTCTTTTGAAGACCCTG
ATAGTGCCGTCGATGACCGAGATAGTGACTATCGCAGTGAGACCAGCAAC
AGCTTCCCACCTCCTTACCATACAGCTTCCCAGCCCAACGCTTCTGTGCA
CCAGTTCCCTGTGCCGGTGCGATCGCCACAGCAGCTGCTACTTCAAGGCA
GTTCCCGGGACTCTTGTAATGACTCTATGCAAAGTTATGACCTTGATTAT
CCAGAGCGGCGGGCTATCAGCCCCACCAGCAGCAGTAGGTATGGCTCCTC
CTGTAATGTGAGTCAAGGAAGCTCTCAGCTAAGTGAACTAGACCAGTATC
ACGAACAAGATGACGACCATCGGGAGACGGACTCGATTCATTCTTGCCAC
AGCTCTCACAGCCTGTCCAGAGATGGCCAAGCAGGTTTTGGAGAACAAGA
GAAACCCTTGGAGGTGACAGGTCAAGCAGAGAAGGAGGCAGCATGTGAAC
CCAAGGAGATGAAAGAAGATGCCACAACCCACCCTCCCCCAGATCTGGTG
CTGCAAAAAGACCACTTCCTAGGTCCCCAGGAGAGTTTTCCTGAGGAGAA
TGCATCTTCACCATTTACCCAAGCCAGAGCACATTGGATCCGAGCAGTTA
CCAAGGTTCGACTCCAGCTGCAGGAGATTCCAGATGATGGTGACCCCTCT
CTGCCTCAGTGGCTCCCGGAAGGGCCAGCCGGAGGGCTCTATGGCATTGA
CAGCATGCCAGATTTACGCAGAAAGAAGCCACTGCCACTTGTCAGTGATC
TGTCACTGGTCCAGTCTCGGAAGGCAGGAATCACTTCTGCAATGGCTACA
CGCACTTCTCTTAAGGACGAAGAGCTGAAATCCCACGTGTATAAGAAAAC
CCTGCAGGCCTTAATCTACCCCATTTCGTGCACCACTCCTCATAACTTTG
AGGTCTGGACGGCCACTACCCCAACCTACTGCTATGAGTGTGAAGGCCTG
CTCTGGGGCATTGCCCGGCAGGGCATGCGCTGCAGCGAATGTGGAGTCAA
GTGCCATGAGAAGTGCCAGGATCTGCTCAATGCTGACTGCCTGCAGCGGG
CTGCAGAAAAGAGCTGTAAACATGGAGCTGAGGACCGGACCCAGAACATT
ATCATGGCCATGAAGGACCGCATGAAGATCCGAGAGCGAAATAAGCCAGA
GATCTTTGAAGTTATCCGGGACGTCTTCACAGTGAACAAAGCTGCCCATG
TGCAGCAGATGAAAACAGTGAAGCAGAGTGTACTGGATGGCACCTCCAAA
TGGTCAGCCAAGATCACCATTACTGTGGTGTGTGCCCAGGGCCTACAAGC
CAAGGACAAAACAGGATCCAGTGACCCTTACGTGACTGTGCAAGTCAGCA
AAACTAAGAAGCGTACCAAGACCATTTTTGGAAATTTGAATCCTGTTTGG
GAGGAGAAGTTCCATTTTGAGTGCCACAACTCCTCTGACCGCATTAAGGT
GCGTGTATGGGATGAGGATGATGACATCAAGTCAAGAGTAAAGCAACGCC
TAAAGCGAGAGTCTGATGATTTCCTTGGCCAAACCATCATTGAGGTTCGG
ACCCTAAGTGGCGAGATGGACGTCTGGTACAACTTGGAGAAGAGGACAGA
CAAATCAGCCGTCTCGGGGGCTATCCGACTACAAATCAGTGTGGAGATCA
AGGGGGAGGAGAAAGTAGCCCCATACCACGTGCAGTATACATGTCTCCAT
GAGAATCTTTTCCATTACCTCACAGACATTCAGGGCAGTGGAGGAGTCCG
CATCCCTGAAGCTCGAGGAGACGATGCCTGGAAGGTGTACTTTGATGAGA
CAGCCCAAGAAATTGTGGATGAATTTGCCATGCGTTATGGCATTGAGTCC
ATATATCAGGCCATGACGCACTTTGCATGTTTATCATCCAAGTACATGTG
TCCTGGTGTGCCAGCAGTGATGAGCACCTTACTGGCCAACATCAACGCCT
ACTATGCCCACACAACTGCCTCTACCAATGTCTCTGCATCTGATCGCTTT
GCAGCCTCCAACTTTGGGAAAGAGAGATTTGTAAAACTGCTGGACCAGCT
ACACAACTCACTGAGGATCGACCTCTCTACATACAGGAATAATTTCCCTG
CTGGGAGTCCTGAACGGCTTCAGGACTTAAAATCCACAGTGGATTTGCTG
ACCAGCATTACTTTCTTCAGAATGAAGGTACAAGAACTGCAAAGCCCTCC
AAGAGCCAGCCAGGTGGTAAAGGATTGTGTGAAGGCCTGTTTGAACTCCA
CATATGAATATATCTTCAACAACTGCCACGACTTATACAGCCGCCAGTAC
CAGCTGAAGCAGGAGCTACCTCCAGAGGAACAAGGGCCCAGCATTCGGAA
CCTGGATTTCTGGCCCAAGCTCATCACACTCATCGTGTCAATCATAGAGG
AAGATAAGAATTCCTACACACCTGTTCTGAACCAGTTTCCTCAGGAGTTG
AATGTGGGAAAAGTCAGCGCAGAAGTGATGTGGCATTTGTTTGCCCAAGA
CATGAAATATGCATTGGAGGAGCATGAGAAAGACCACCTGTGTAAAAGTG
CTGACTACATGAACCTGCACTTCAAGGTGAAGTGGCTCCACAATGAATAC
GTGCGGGATCTGCCTGTCCTCCAGGGGCAGGTGCCTGAGTACCCAGCGTG
GTTTGAGCAGTTCGTGCTACAATGGCTGGATGAGAATGAGGATGTATCCC
TGGAATTCCTGCGTGGGGCCCTGGAACGAGATAAGAAGGATGGATTCCAG
CAGACATCAGAGCATGCACTCTTTTCCTGCTCTGTGGTGGATGTCTTCAC
ACAACTCAATCAGAGCTTTGAGATCATCCGGAAGCTGGAATGCCCAGACC
CCAGCATCCTTGCCCACTACATGAGGAGGTTTGCTAAGACCATCGGGAAG
GTGCTGATGCAGTATGCAGACATCTTGTCAAAGGACTTCCCAGCCTATTG
CACAAAGGAGAAACTGCCCTGCATCCTGATGAACAACGTGCAGCAACTGA
GGGTCCAGCTGGAGAAAATGTTTGAGGCCATGGGAGGCAAGGAGCTGGAC
CTTGAAGCTGCAGACAGTCTGAAGGAGCTGCAGGTGAAACTGAATACGAT
TCTGGATGAGCTCAGCATGGTGTTTGGAAACAGTTTCCAGGTACGGATTG
ATGAGTGTGTTCGACAAATGGCCGACATCCTGGGCCAGGTTCGGGGCACA
GGGAATGCATCTCCAGACGCCAGGGCCTCAGCGGCTCAGGATGCAGATAG
CGTACTCCGGCCTCTCATGGACTTCCTGGATGGCAACCTCACCCTCTTTG
CCACTGTGTGTGAGAAGACGGTTCTGAAGCGTGTACTGAAGGAGCTCTGG
CGCGTGGTGATGAACACAATGGAGAGGATGATTGTTCTGCCCCCACTCAC
TGACCAGACGGGCACCCAGCTGATCTTCACTGCTGCCAAGGAGCTGAGCC
ATCTTTCCAAACTCAAGGATCACATGGTACGAGAGGAAACACGGAATCTC
ACTCCAAAGCAGTGTGCAGTCCTTGACCTCGCCCTGGACACCATCAAGCA
ATACTTCCATGCAGGAGGCAATGGGCTGAAGAAAACCTTCCTGGAGAAGA
GCCCAGATCTGCAGTCTCTACGCTATGCCCTGTCTCTGTACACACAGACT
ACTGACACTCTCATCAAGACCTTTGTGCGCTCGCAGACCACCCAAGTGCA
TGATGGGAAAGGTATTAGATTTACTGCTAATGAGGACATTCGTCCGGAAA
AGGGGTCTGGTGTGGACGATCCTGTGGGAGAAGTCTCTATTCAGGTGGAC
TTGTTTACACACCCTGGTACTGGGGAGCACAAGGTCACAGTGAAAGTGGT
GGCTGCCAATGACCTCAAGTGGCAGACAGCGGGTATGTTCCGGCCTTTCG
TGGAGGTGACTATGGTTGGCCCACACCAAAGTGATAAGAAGAGGAAGTTC
ACAACCAAATCCAAAAGCAACAACTGGGCCCCCAAGTACAATGAGACATT
CCACTTCCTCCTGGGAAATGAGGAGGGGCCCGAGTCCTATGAGTTGCAGA
TATGCGTGAAGGATTACTGCTTTGCCCGGGAAGATCGCGTGCTAGGGCTG
GCTGTGATGCCTCTGAGGGATGTCACAGCCAAGGGCAGCTGTGCCTGCTG
GTGCCCCTTGGGCCGGAAGATCCATATGGATGAGACAGGCCTGACCATTC
TCCGGATTTTATCTCAGAGGAGCAATGACGAGGTGGCCCGAGAATTTGTG
AAACTCAAATCAGAGTCTCGTTCCACGGAGGAGGGGAGCTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPGSSRKG
TIDAAAMSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPS
WEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPG
EWSTLEAETLMKDDEICGTRNPTPHKILLDTRFELPFDIPEEEARYWTYK
WEQINALGADNEYSSQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSE
TSNSFPPPYHTASQPNASVHQFPVPVRSPQQLLLQGSSRDSCNDSMQSYD
LDYPERRAISPTSSSRYGSSCNVSQGSSQLSELDQYHEQDDDHRETDSIH
SCHSSHSLSRDGQAGFGEQEKPLEVTGQAEKEAACEPKEMKEDATTHPPP
DLVLQKDHFLGPQESFPEENASSPFTQARAHWIRAVTKVRLQLQEIPDDG
DPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSA
MATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYEC
EGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSCKHGAEDRT
QNIIMAMKDRMKIRERNKPEIFEVIRDVFTVNKAAHVQQMKTVKQSVLDG
TSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVSKTKKRTKTIFGNLN
PVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTII
EVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYT
CLHENLFHYLTDIQGSGGVRIPEARGDDAWKVYFDETAQEIVDEFAMRYG
IESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSAS
DRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTV
DLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYS
RQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLNQFP
QELNVGKVSAEVMWHLFAQDMKYALEEHEKDHLCKSADYMNLHFKVKWLH
NEYVRDLPVLQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKD
GFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPSILAHYMRRFAKT
IGKVLMQYADILSKDFPAYCTKEKLPCILMNNVQQLRVQLEKMFEAMGGK
ELDLEAADSLKELQVKLNTILDELSMVFGNSFQVRIDECVRQMADILGQV
RGTGNASPDARASAAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLK
ELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREET
RNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLY
TQTTDTLIKTFVRSQTTQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSI
QVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKK
RKFTTKSKSNNWAPKYNETFHFLLGNEEGPESYELQICVKDYCFAREDRV
LGLAVMPLRDVTAKGSCACWCPLGRKIHMDETGLTILRILSQRSNDEVAR
EFVKLKSESRSTEEGS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16734
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee