cDNA Clone UBE2E3

• Plasmid: pGEX6P-2-UBE2E3
• Parent Plasmid: pGEX6P2
• Expressed: GST-UBE2E3
• Genbank: NM_006357.2 GI:33359695
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGTCCAGTGATAGGCAAAGGTCCGATGATGAGAGCCCCAGCAC
CAGCAGTGGCAGTTCAGATGCGGACCAGCGAGACCCAGCCGCTCCAGAGC
CTGAAGAACAAGAGGAAAGAAAACCTTCTGCCACCCAGCAGAAGAAAAAC
ACCAAACTCTCTAGCAAAACCACTGCTAAGTTATCCACTAGTGCTAAAAG
AATTCAGAAGGAGCTAGCTGAAATAACCCTTGATCCTCCTCCTAATTGCA
GTGCTGGGCCTAAAGGAGATAACATTTATGAATGGAGATCAACTATACTT
GGTCCACCGGGTTCTGTATATGAAGGTGGTGTGTTTTTTCTGGATATCAC
ATTTTCATCAGATTATCCATTTAAGCCACCAAAGGTTACTTTCCGCACCA
GAATCTATCACTGCAACATCAACAGTCAGGGAGTCATCTGTCTGGACATC
CTTAAAGACAACTGGAGTCCCGCTTTGACTATTTCAAAGGTTTTGCTGTC
TATTTGTTCCCTTTTGACAGACTGCAACCCTGCGGATCCTCTGGTTGGAA
GCATAGCCACTCAGTATTTGACCAACAGAGCAGAACACGACAGGATAGCC
AGACAGTGGACCAAGAGATACGCAACATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSSDRQRSDDESPSTSSGS
SDADQRDPAAPEPEEQEERKPSATQQKKNTKLSSKTTAKLSTSAKRIQKE
LAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSD
YPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSL
LTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYAT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31146
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 49735 Daltons